Seiko Yamano
Yamaguchi University
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Featured researches published by Seiko Yamano.
Equine Veterinary Journal | 2010
Daisuke Eto; Seiko Yamano; Yoshinori Kasashima; Takao Sugiura; T. Nasu; M. Tokuriki; Hirofumi Miyata
REASONS FOR PERFORMING STUDY Most racehorses are trained regularly from about age 18 months; therefore, little information is available on the effect of training in Thoroughbred foals. HYPOTHESIS Well-controlled exercise could improve muscle potential ability for endurance running. METHODS Thoroughbred foals at age 2 months were separated into control and training (treadmill exercise) groups and samples obtained from the middle gluteal muscle at 2 and 12 months post partum. Muscle fibre compositions were determined by histochemical and electrophoretical techniques and succinic dehydrogenase (SDH) activity was analysed in each fibre type. RESULTS All fibre types were hypertrophied with growth and type I and IIA fibres were significantly larger in the training than the control group at age 12 months. A significant increase of SDH activity was found in type IIX muscle fibres in the training group. CONCLUSIONS Training in young Thoroughbred horses can facilitate muscle fibre hypertrophy and increase the oxidative capacity of type IIX fibres, which could potentially enhance stamina at high speeds. POTENTIAL RELEVANCE To apply this result to practical training, further studies are needed to determine more effective and safe intensities of controlled exercise.
Journal of Equine Science | 2010
Seiko Yamano; Minako Kawai; Yoshio Minami; Atsushi Hiraga; Hirofumi Miyata
We evaluated differences in muscle fiber recruitment patterns between continuous and interval training to develop an optimal training program for Thoroughbred horses. Five well trained female thoroughbred horses (3–4 years old) were used. The horses performed two different exercises on a 10% inclined treadmill: 90%VO2 max for 4 min (continuous) and 90% VO2 max for 2 min × 2 times with 10-min interval (interval). Muscle samples were obtained from the middle gluteal muscle before and immediately after the exercises. Four muscle fiber types (type I, IIA, IIA/X, and IIX) were immunohistochemically identified, and the optical density of periodic acid Schiff staining (OD-PAS) in each fiber type and glycogen content of the muscle sample were determined by quantitative histochemical and biochemical procedures, respectively. No significant differences were found in the OD-PASs and glycogen contents between the continuous and interval exercises, but the decreases in OD-PAS of fast-twitch muscle fibers were obvious after interval as compared to continuous exercise. Interval exercise may be a more effective training stimulus for the glycolytic capacity of fast-twitch muscle fiber. The data about muscle fiber recruitment can provide significant insights into the optimal training program not only for thoroughbred horses, but also for human athletes.
Journal of Equine Science | 2009
Yoshio Minami; Seiko Yamano; Minako Kawai; Atsushi Hiraga; Hirofumi Miyata
To find a new parameter indicating muscle fitness in Thoroughbred horses, we examined time-dependent recovery of glycogen content and sarcoplasmic reticulum (SR) Ca2+-ATPase activity of skeletal muscle after intensive treadmill running. Two repeated 50-sec running sessions (13 m/sec) were performed on a flat treadmill (approximately 90%VO2max). Muscle samples of the middle gluteal muscle were taken before exercise (pre) and 1 min, 20 min, 60 min, and 24 hr after exercise. Muscle fiber type composition was determined in the pre muscle samples by immunohistochemical staining with monoclonal antibody to myosin heavy chain. SR Ca2+-ATPase activity of the muscle and glycogen content of each muscle fiber type were determined with biochemical analysis and quantitative histochemical staining, respectively. As compared to the pre value, the glycogen content of each muscle fiber type was reduced by 15–27% at 1 min, 20 min, and 60 min after the exercise and recovered to the pre value at 24 hr after exercise test. These results indicate that 24 hr is enough time to recover glycogen content after short-term intensive exercise. The mean value of the SR Ca2+-ATPase activity showed a slight decrease (not significant) immediately after exercise, and complete recovery at 60 min after exercise. There were no significant relationship between the changes in glycogen content of each muscle fiber type and SR Ca2+-ATPase. Although further studies are needed, SR Ca2+-ATPase is not a useful parameter to detect muscle fitness, at least in Thoroughbred horses.
American Journal of Veterinary Research | 2005
Seiko Yamano; Daisuke Eto; Yoshinori Kasashima; Atushi Hiraga; Takao Sugiura; Hirofumi Miyata
Research in Veterinary Science | 2004
Daisuke Eto; Seiko Yamano; Kazutaka Mukai; Takao Sugiura; T. Nasu; M. Tokuriki; Hirofumi Miyata
American Journal of Veterinary Research | 2002
Seiko Yamano; Daisuke Eto; Takao Sugiura; Makoto Kai; Atsushi Hiraga; Mikihiko Tokuriki; Hirofumi Miyata
Research in Veterinary Science | 2006
Seiko Yamano; Daisuke Eto; Atsushi Hiraga; Hirofumi Miyata
Biomedical Research-tokyo | 2009
Hidetaka Imagita; Seiko Yamano; Yoshiko Tobimatsu; Hirofumi Miyata
Equine Veterinary Journal | 2006
Daisuke Eto; Seiko Yamano; Atsushi Hiraga; Hirofumi Miyata
Japanese Journal of Physical Fitness and Sports Medicine | 2005
Emiko Shono; Seiko Yamano; Hirofumi Miyata