Hiroko Nakamori

Anomalous partition behaviour of sodium dodecyl sulphate monomer in sephadex gels with high dextran concentrations : Possible role of water of hydration in the gel phase

Abstract The partition coefficient of sodium dodecyl sulphate (SDS) monomer for Sephadex gels with high dextran concentrations were found to be abnormally high, and this was attributed to the anomalous nature of the internal water of Sephadex gel stemming from strong hydration. The thermodynamic parameters relevant to the transfer of SDS from external water to internal water indicate that the preferential partition of SDS monomer is a hydrophobic free energy driven process. “Iceberg” formation is partially prevented in the water of hydration, thereby allowing the preferential partition of SDS without an excessive increase in free energy.