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Dive into the research topics where Hiromichi Yokoi is active.

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Featured researches published by Hiromichi Yokoi.


FEBS Letters | 2002

Molecular cloning and characterization of Kv6.3, a novel modulatory subunit for voltage‐gated K+ channel Kv2.11

Yorikata Sano; Shinobu Mochizuki; Akira Miyake; Chika Kitada; Kohei Inamura; Hiromichi Yokoi; Katsura Nozawa; Hitoshi Matsushime; Kiyoshi Furuichi

We report identification and characterization of Kv6.3, a novel member of the voltage‐gated K+ channel. Reverse transcriptase‐polymerase chain reaction analysis indicated that Kv6.3 was highly expressed in the brain. Electrophysiological studies indicated that homomultimeric Kv6.3 did not yield a functional voltage‐gated ion channel. When Kv6.3 and Kv2.1 were co‐expressed, the heteromultimeric channels displayed the decreased rate of deactivation compared to the homomultimeric Kv2.1 channels. Immunoprecipitation studies indicated that Kv6.3 bound with Kv2.1 in co‐transfected cells. These results indicate that Kv6.3 is a novel member of the voltage‐gated K+ channel which functions as a modulatory subunit.


Journal of Biological Chemistry | 1999

New Ether-à-go-go K+ Channel Family Members Localized in Human Telencephalon

Akira Miyake; Shinobu Mochizuki; Hiromichi Yokoi; Masanobu Kohda; Kiyoshi Furuichi

A cDNA encoding a novel voltage-gated K+ channel protein was isolated from human brain. This protein, termed BEC1, is 46% identical to rat elk in theether-à-go-go K+ channel family. TheBEC1 gene maps to the 12q13 region of the human genome. Northern blot analysis indicates that BEC1 is exclusively expressed in human brain, where the expression is concentrated in the telencephalic areas such as the cerebral cortex, amygdala, hippocampus, and striatum. By in situ hybridization, BEC1 is detected in the CA1–CA3 pyramidal cell layers and the dentate gyrus granule cell layers of the hippocampus. Specific signals are also found in neocortical neurons. Transfection of mammalian L929 and Chinese hamster ovary cells with BEC1 cDNA induces a voltage-gated outward current with a fast inactivation component. This current is insensitive to tetraethylammonium and quinidine. Additionally, a second related gene BEC2 was isolated from human brain. BEC2 is also brain-specific, located in the neocortex and the striatum, and functional as a channel gene. Phylogenetic analysis indicates that BEC1 andBEC2 constitute a subfamily, together with elk, in the ether-à-go-go family. The two genes may be involved in cellular excitability of restricted neurons in the human central nervous system.


Science | 2001

Immunocyte Ca2+ Influx System Mediated by LTRPC2

Yorikata Sano; Kohei Inamura; Akira Miyake; Shinobu Mochizuki; Hiromichi Yokoi; Hitoshi Matsushime; Kiyoshi Furuichi


Journal of Biological Chemistry | 2003

A novel two-pore domain K+ channel, TRESK, is localized in the spinal cord

Yorikata Sano; Kohei Inamura; Akira Miyake; Shinobu Mochizuki; Chika Kitada; Hiromichi Yokoi; Katsura Nozawa; Hidetsugu Okada; Hitoshi Matsushime; Kiyoshi Furuichi


Archive | 2001

Brain specific potassium channel protein

Shinobu Mochizuki; Hiromichi Yokoi


Archive | 1999

NOVEL POTASSIUM CHANNEL PROTEIN

Shinobu Mochizuki; Hiromichi Yokoi


Archive | 2001

Novel potassium channel

Hiromichi Yokoi; Kohei Inamura; Yorikata Sano; Shinobu Mochizuki


Archive | 2001

Methods of screening for modifiers of potassium channel protein activity

Shinobu Mochizuki; Hiromichi Yokoi


Archive | 2002

Method of screening cell death inhibitor

Yorikata Sano; Kohei Inamura; Hiromichi Yokoi; Katsura Nozawa; Shinobu Mochizuki


Archive | 2002

Procede de criblage d'un inhibiteur de la mort cellulaire

Yorikata Sano; Kohei Inamura; Hiromichi Yokoi; Katsura Nozawa; Shinobu Mochizuki

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Akira Miyake

University of Colorado Boulder

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Akira Miyake

University of Colorado Boulder

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