Hirosi Sato

Chemistry and biology of phospholipids from an unclassified mycobacteria, P6

Abstract Crude phospholipid fraction from P6 (Scotochromogen) prepared by extraction with chloroform: methanol (2:1), followed by removal of non-lipid contaminants by Folchs procedure and extraction with acetone, yielded three fractions by column chromatography with silicic acid. These fractions were designated as fractions I, II and III in the decreasing order of their Rfs on thin layer plates of Silica Gel. Fraction I was identified as diphosphatidyl glycerol (cardiolipin) and was antigenic in flocculation test for syphilis and in latex agglutination test for lepromatous leprosy. Fraction II was identified as phosphatidyl ethanolamine and Fraction III as phosphatidyl inositol monomannoside.

Pattern of fibronectin distribution in human lung cancer.

SummaryThe pattern of fibronectin (FN) distribution in human lung cancer was studied by indirect immunofluorescent staining, and by the peroxidase-antiperoxidase method in a total of 60 surgical specimens. They comprised 8 small cell carcinomas, 4 large cell carcinomas, 19 squamous cell carcinomas, 28 adenocarcinomas, and 1 adenoid cystic carcinoma.Of the 60 specimens 13 were FN-positive. They included 4 large cell carcinomas, 4 small cell carcinomas, 3 poorly differentiated squamous cell carcinomas, and 2 poorly differentiated and 1 moderatety differentiated adenocarcinomas.On the other hand, none of the well differentiated carcinomas was FN-positive around tumor cells. Our data suggest that undifferentiated, or poorly differentiated carcinomas of the lung tend to be FN-positive.

Glycosaminoglycans and glycoproteins in bronchoalveolar lavage fluid from patients with pulmonary alveolar proteinosis.

Bronchoalveolar lavage fluid from two cases of pulmonary alveolar proteinosis were analyzed for glycosaminoglycans and glycoproteins. The clinical courses of the two cases were entirely different. In one patient, signs and symptoms recurred despite repeated therapeutic bronchoalveolar lavages. In the other patient, three successive bronchoalveolar lavages brought about complete recovery. It was found that the bronchoalveolar lavage fluid from the former case contained various subtypes of glycosaminoglycans [hyaluronic acid, chondroitin sulfate A(C), dermatan sulfate, and heparan sulfate] and glycoprotein. On the other hand, the bronchoalveolar lavage fluid from the latter case contained glycoprotein, but no detectable amounts of glycosaminoglycans. There was only a slight qualitative difference in glycoprotein of bronchoalveolar lavage fluid between the two cases. The presence or absence of glycosaminoglycans in bronchoalveolar lavage fluid may be related to the prognosis of pulmonary alveolar proteinosis.

Acid glycosaminoglycans in experimental pleural effusions.

Acid glycosaminoglycans (GAG) were quantitated and partially characterized in pleural effusions in guinea pigs with experimental allergic pleurisy. (a) Increase of GAG in the pleural effusion preceded the increase of effusion volume. Effusion was associated with the presence of cuboidal mesothelial cells on visceral pleura, and hemorrhage, infiltration of neutrophils, and destruction of the alveolar structure of the lung. (b) A significant difference was found in the relative percentage of GAG subtypes in the effusion, serum, and the lung tissue of guinea pigs with experimental pleurisy. (c) There was a difference in molecular size of hyaluronic acid in pleural effusion between the early and late stages of pleurisy. (d) Presumably GAGs found in pleural effusion were released from the pleural tissue.