Sutemi Oka

Chemistry and biology of phospholipids from an unclassified mycobacteria, P6

Abstract Crude phospholipid fraction from P6 (Scotochromogen) prepared by extraction with chloroform: methanol (2:1), followed by removal of non-lipid contaminants by Folchs procedure and extraction with acetone, yielded three fractions by column chromatography with silicic acid. These fractions were designated as fractions I, II and III in the decreasing order of their Rfs on thin layer plates of Silica Gel. Fraction I was identified as diphosphatidyl glycerol (cardiolipin) and was antigenic in flocculation test for syphilis and in latex agglutination test for lepromatous leprosy. Fraction II was identified as phosphatidyl ethanolamine and Fraction III as phosphatidyl inositol monomannoside.

Action Mechanism of 3,4-Dideoxykanamycin B (DKB) on Some Gram-Negative Bacteria

A new antibiotic, 3′,4′-dideoxykanamycin B, abbreviated as DKB, has a broad spectrum of antibacterial activity with a strong selective inhibition on Pseudomonas aeruginosa and kanamycin-resistant Escherichia coli. To study the action modes of DKB, this research was done on the basis of the ultramicroscopic structures and the polypeptide synthesis of the two following bacilli, which had been exposed to DKB.