Hisayuki Ojima

Two classes of cortical neurones labelled with Vicia villosa lectin in the guinea-pig

Cell surface substance(s) containing terminal N-acetylgalactosamine (GaINAc) was localized in the cerebral cortex of the guinea-pig. Vicia villosa and Glycine max, both specific to GaINAc, labelled the surface of the cell body of multipolar and triangular neurones. The labelling extended to the apical dendrite and axon initial segment in the triangular neurones. They were distributed exclusively in layer 5. Most of the labelled multipolar neurones contained a calcium-binding protein, parvalbumin (Pv), while none of the triangular neurones did. The findings indicate that, in the guinea-pig, pyramidal neurones as well as non-pyramidal interneurones are enwrapped by GaINAc-containing substances and suggest that this enwrapping does not seem to be linked to Pv production.

Interplay of excitation and inhibition elicited by tonal stimulation in pyramidal neurons of primary auditory cortex

Tonal responses of neurons in the primary auditory cortex are a function of frequency, intensity and ear of stimulation. These responses occasionally display suppression. This review discusses how excitatory and inhibitory synaptic inputs interact to form suppressive responses and how changes in stimulus attributes affect the magnitude and timing of those responses. Stimulation at the characteristic frequency evokes a stereotyped sequence of depolarization (excitatory) and then hyperpolarization (inhibitory), as predicted from the canonical circuitry. Some neurons stimulated at higher sound intensities display a prominent increase in the magnitude of hyperpolarization or a decrease in its latency, both enabling counteraction with the preceding excitation. These interactions, in part, underlie the non-monotonic suppression. Furthermore, monaural non-dominant ear stimulation elicits such a powerful hyperpolarization as to cancel out the depolarization elicited at dominant ear stimulation, suggesting a linear mechanism for the binaural suppression. Alternatively, it elicits a depolarization almost equal in magnitude and time course to that elicited at binaural stimulation, suggesting a nonlinear interaction responsible for the suppression. Laminar differences are also noted for these inhibitory interactions.

Neural response to movement of the hand and mouth in the secondary somatosensory cortex of Japanese monkeys during a simple feeding task.

Abstract Neural activity was recorded in the secondary somatosensory cortex (SII) of macaque monkeys during a simple feeding task. Around the border between the representations of the hand and face in SII, we found neurons that became active during both retrieving with the hand and eating; 59% had receptive fields (RFs) in the hand/face and the remaining 41% had no RFs. Neurons that responded to touching objects were rarely found. This suggests their sensorimotor function rather than tactile object recognition.

Use of two anterograde axon tracers to label distinct cortical neuronal populations located in close proximity.

In order to investigate converging projections originating from adjacent populations of cortical neurons, injections of two different anterograde tracers, biotinylated dextranamine (BDA) and Phaseolus vulgaris leucoagglutinin (PHA-L), were made in close proximity. When the two injection sites were separated by around 500 microm and the time between injections was 1--4 h, BDA-labeling of neuronal elements was found not only at the BDA injection site but also at the PHA-L injection site. This false-positive BDA labeling of neurons at the PHA-L injection site was so intense that labeled axons could be traced, both into the neighboring cortical gray matter and into white matter. Increasing the separation distance to 1000 microm resulted in much fewer falsely positive labeled neurons at the PHA-L injection site. Even more effective was extending the time interval between the two injections. Thus, if the BDA injection preceded the PHA-L injection by more than 12 h, virtually no false-positive labeling was associated with the PHA-L injection site. These procedures may be applied to other combinations of anterograde tracers, such as BDA with tetramethylrhodamine-conjugated dextran amine.

Auditory Cortical Projections to the Medial Geniculate Body

Although the thalamus is traditionally regarded as a simple relay station for sensory information reaching cerebral cortex from the periphery, growing evidence supports a new view: that it actively regulates the passage of sensory information and modulates sensory signals. These modulations are enabled by inhibitory circuits, ascending inputs from brain stem nuclei, and corticofugal projections. Descending inputs to the thalamus are dual in terms of cells of origin and their target, acting as “drivers” and “modulators”. This chapter describes morphological characteristics, distribution, synaptic formation, and functional properties of the dual corticothalamic projections.

Adaptive Changes in Firing of Primary Auditory Cortical Neurons following Illumination Shift from Light to Dark in Freely Moving Guinea Pigs

Some animals are forced to rely more on non-visual signals, such as audition or olfaction, than on vision when a bright environment becomes dark. By recording from a primary-like auditory cortex (field A) in freely moving guinea pigs, possible changes in the responsiveness of single units were explored in association with illumination changes. For a subset of units, we found that robust decreases (off-decrease) or increases (off-increase) in baseline discharge (BsD) were initiated soon after room light was silently extinguished. These neuronal changes were accompanied by the initiation of explorative locomotion, possibly reflecting a changed internal brain state. Preferred acoustic stimuli evoked salient excitatory responses against the reduced BsD level in the dark for the off-decrease units, and salient inhibitory responses against the increased BsD level for the off-increase units. Histological verification indicated that the units showing such BsD changes were located predominantly in layer V or its vicinity. These results are discussed in the context of the effects of the brainstem neuromodulatory systems that are activated during behavioral adaptation to new environments.

Recognition of Non-Harmonic Natural Sounds by Small Mammals Using Competitive Training

Animals recognize biologically relevant sounds, such as the non-harmonic sounds made by some predators, and respond with adaptive behaviors, such as escaping. To clarify which acoustic parameters are used for identifying non-harmonic, noise-like, broadband sounds, guinea pigs were conditioned to a natural target sound by introducing a novel training procedure in which 2 or 3 guinea pigs in a group competed for food. A set of distinct behavioral reactions was reliably induced almost exclusively to the target sound in a 2-week operant training. When fully conditioned, individual animals were separately tested for recognition of a set of target-like sounds that had been modified from the target sound, with spectral ranges eliminated or with fine or coarse temporal structures altered. The results show that guinea pigs are able to identify the noise-like non-harmonic natural sounds by relying on gross spectral compositions and/or fine temporal structures, just as birds are thought to do in the recognition of harmonic birdsongs. These findings are discussed with regard to similarities and dissimilarities to harmonic sound recognition. The results suggest that similar but not identical processing that requires different time scales might be used to recognize harmonic and non-harmonic sounds, at least in small mammals.

Auditory-visual integration in fields of the auditory cortex

Abstract While multimodal interactions have been known to exist in the early sensory cortices, the response properties and spatiotemporal organization of these interactions are poorly understood. To elucidate the characteristics of multimodal sensory interactions in the cerebral cortex, neuronal responses to visual stimuli with or without auditory stimuli were investigated in core and belt fields of guinea pig auditory cortex using real‐time optical imaging with a voltage‐sensitive dye. On average, visual responses consisted of short excitation followed by long inhibition. Although visual responses were observed in core and belt fields, there were regional and temporal differences in responses. The most salient visual responses were observed in the caudal belt fields, especially posterior (P) and dorsocaudal belt (DCB) fields. Visual responses emerged first in fields P and DCB and then spread rostroventrally to core and ventrocaudal belt (VCB) fields. Absolute values of positive and negative peak amplitudes of visual responses were both larger in fields P and DCB than in core and VCB fields. When combined visual and auditory stimuli were applied, fields P and DCB were more inhibited than core and VCB fields beginning approximately 110 ms after stimuli. Correspondingly, differences between responses to auditory stimuli alone and combined audiovisual stimuli became larger in fields P and DCB than in core and VCB fields after approximately 110 ms after stimuli. These data indicate that visual influences are most salient in fields P and DCB, which manifest mainly as inhibition, and that they enhance differences in auditory responses among fields. HighlightsCross‐modal visual responses were observed in all fields of the auditory cortex.The responses consisted of short excitation followed by long inhibition.Visual influences were inhibition dominant and dependent on regions.The most salient visual influences were observed in dorsocaudal belt fields.Response differences among fields became larger with combined audiovisual stimuli.

Recognition of Modified Conditioning Sounds by Competitively Trained Guinea Pigs

The guinea pig (GP) is an often-used species in hearing research. However, behavioral studies are rare, especially in the context of sound recognition, because of difficulties in training these animals. We examined sound recognition in a social competitive setting in order to examine whether this setting could be used as an easy model. Two starved GPs were placed in the same training arena and compelled to compete for food after hearing a conditioning sound (CS), which was a repeat of almost identical sound segments. Through a 2-week intensive training, animals were trained to demonstrate a set of distinct behaviors solely to the CS. Then, each of them was subjected to generalization tests for recognition of sounds that had been modified from the CS in spectral, fine temporal and tempo (i.e., intersegment interval, ISI) dimensions. Results showed that they discriminated between the CS and band-rejected test sounds but had no preference for a particular frequency range for the recognition. In contrast, sounds modified in the fine temporal domain were largely perceived to be in the same category as the CS, except for the test sound generated by fully reversing the CS in time. Animals also discriminated sounds played at different tempos. Test sounds with ISIs shorter than that of the multi-segment CS were discriminated from the CS, while test sounds with ISIs longer than that of the CS segments were not. For the shorter ISIs, most animals initiated apparently positive food-access behavior as they did in response to the CS, but discontinued it during the sound-on period probably because of later recognition of tempo. Interestingly, the population range and mean of the delay time before animals initiated the food-access behavior were very similar among different ISI test sounds. This study, for the first time, demonstrates a wide aspect of sound discrimination abilities of the GP and will provide a way to examine tempo perception mechanisms using this animal species.

Cortical Activation Patterns Evoked by Temporally Asymmetric Sounds and Their Modulation by Learning

Visual Abstract When complex sounds are reversed in time, the original and reversed versions are perceived differently in spectral and temporal dimensions despite their identical duration and long-term spectrum-power profiles. Spatiotemporal activation patterns evoked by temporally asymmetric sound pairs demonstrate how the temporal envelope determines the readout of the spectrum. We examined the patterns of activation evoked by a temporally asymmetric sound pair in the primary auditory field (AI) of anesthetized guinea pigs and determined how discrimination training modified these patterns. Optical imaging using a voltage-sensitive dye revealed that a forward ramped-down natural sound (F) consistently evoked much stronger responses than its time-reversed, ramped-up counterpart (revF). The spatiotemporal maximum peak (maxP) of F-evoked activation was always greater than that of revF-evoked activation, and these maxPs were significantly separated within the AI. Although discrimination training did not affect the absolute magnitude of these maxPs, the revF-to-F ratio of the activation peaks calculated at the location where hemispheres were maximally activated (i.e., F-evoked maxP) was significantly smaller in the trained group. The F-evoked activation propagated across the AI along the temporal axis to the ventroanterior belt field (VA), with the local activation peak within the VA being significantly larger in the trained than in the naïve group. These results suggest that the innate network is more responsive to natural sounds of ramped-down envelopes than their time-reversed, unnatural sounds. The VA belt field activation might play an important role in emotional learning of sounds through its connections with amygdala.