Hitoshi Shigematsu
Japan Tobacco
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Featured researches published by Hitoshi Shigematsu.
Agricultural and biological chemistry | 1991
Yasuhiro Shinozaki; Toshiake Matsuzaki; Shizuo Suhara; Tetsuya Tobita; Hitoshi Shigematsu; Akira Koiwai
The surface lipids of Nicotiana umbratica contained several varieties of glycolipids. Three types of glucose esters and five types of sucrose esters were isolated and identified from this species. These glycolipids contained short-chain fatty acids such as acetic, methylpropionic, methylbutyric, 3-methylpentanoic, 4-methylpentanoic and methylhexanoic acids. The acetylated positions of each compound were determined by 2-D NMR, using the HMBC technique. The structures of the glucose esters were 1,6-di-O-acetyl-2,3-4-tri-O-acyl-alpha-D-glucopyranose (I), 6-O-acetyl-2,3,4-tri-O-acyl-alpha-D-glucopyranose (II) and 2,3,4-tri-O-acyl-alpha, beta-D-glucopyranose (III). The structures of the sucrose esters were 6-O-acetyl-2,3,4-tri-O-acyl-alpha-D-glucopyranosyl-1,3-di-O-acetyl-beta- D- fructofuranoside (IV), 2,3,4-tri-O-acyl-alpha-D-glucopyranosyl-1,3-di-O-acetyl-beta-D-fructofur anoside (V), 6-O-acetyl-2,4-di-O-acyl-alpha-D-glucopyranosyl-3-O-acetyl-beta-D- fructofuranoside (VI), 2,3,4-tri-O-acyl-alpha-D-glucopyranosyl-3-O-acetyl-beta-D-fructofuranosi de 2,3,4-tri-O-acyl-alpha-D-glucopyranosyl-3-O-acetyl-beta-D-fructofuranosi de (VII) and 2,3,4-tri-O-acyl-alpha-D-glucopyranosyl-beta-D-fructofuranoside (VIII). Among them, I, IV, V and VI were isolated for the first time from plants of the family Solanaceae. These newly isolated glycolipids were inhibitory against barnyardgrass growth.
Agricultural and biological chemistry | 1989
Toshiake Matsuzaki; Yasuhiro Shinozaki; Shizuo Suhara; Hitoshi Shigematsu; Akira Koiwai
Isolation and characterization of homologous glucolipids with tetra- or triacyclglucose structures from the surface lipids of N. miersii and their plant growth inhibitory activity
Archive | 1992
Shinji Sakamoto; Kazumasa Tsuji; Kyoko Uehara; Michiko Aoki; Masaharu Iijima; Hitoshi Shigematsu; Hiroshi Matsuzawa; Takahisa Ohta
Aqualysin I (AQI) is a subtilisin-type serine protease which is secreted into the culture medium by Thermus aquaticus YT-1, an extreme thermophile. AQI is considerably heat-stable and resistant to denaturing treatments in comparison with other microbial serine proteases. Terada et al. previously reported the production of the enzymatically active AQI by expressing the AQI gene under the control of the tac promoter in Escherichia coli [1]. In this study, to achieve efficient production of AQI, batch and fed-batch cultures of E.coli TG1 transformed with plasmid pAQN containing the AQI gene were investigated.
Journal of Agricultural and Food Chemistry | 1975
Hitoshi Shigematsu; Saizo Shibata; Tadao Kurata; Hiromichi Kato; Masao Fujimaki
Bioscience, Biotechnology, and Biochemistry | 1992
Toshiake Matsuzaki; Yasuhiro Shinozaki; Manabu Hagimori; Tetsuya Tobita; Hitoshi Shigematsu; Akira Koiwai
Agricultural and biological chemistry | 1977
Hitoshi Shigematsu; Saizo Shibata; Tadao Kurata; Hiromichi Kato; Masao Fujimaki
Agricultural and biological chemistry | 1972
Hitoshi Shigematsu; Tadao Kurata; Hiromichi Kato; Masao Fujimaki
Agricultural and biological chemistry | 1971
Hitoshi Shigematsu; Tadao Kurata; Hiromichi Kato; Masao Fujimaki
Agricultural and biological chemistry | 1987
Katsuya Fukuhara; Takane Fujimori; Hitoshi Shigematsu; Akio Ohnishi
Agricultural and biological chemistry | 1972
Hiromichi Kato; Hitoshi Shigematsu; Tadao Kurata; Masao Fujimaki