Hongen Jiang

Phytochemical and genetic analyses of ancient cannabis from Central Asia

The Yanghai Tombs near Turpan, Xinjiang-Uighur Autonomous Region, China have recently been excavated to reveal the 2700-year-old grave of a Caucasoid shaman whose accoutrements included a large cache of cannabis, superbly preserved by climatic and burial conditions. A multidisciplinary international team demonstrated through botanical examination, phytochemical investigation, and genetic deoxyribonucleic acid analysis by polymerase chain reaction that this material contained tetrahydrocannabinol, the psychoactive component of cannabis, its oxidative degradation product, cannabinol, other metabolites, and its synthetic enzyme, tetrahydrocannabinolic acid synthase, as well as a novel genetic variant with two single nucleotide polymorphisms. The cannabis was presumably employed by this culture as a medicinal or psychoactive agent, or an aid to divination. To our knowledge, these investigations provide the oldest documentation of cannabis as a pharmacologically active agent, and contribute to the medical and archaeological record of this pre-Silk Road culture.

Proteomics identifies the composition and manufacturing recipe of the 2500-year old sourdough bread from Subeixi cemetery in China.

UNLABELLED We report on the geLC-MS/MS proteomics analysis of cereals and cereal food excavated in Subeixi cemetery (500-300BC) in Xinjiang, China. Proteomics provided direct evidence that at the Subexi sourdough bread was made from barley and broomcorn millet by leavening with a renewable starter comprising bakers yeast and lactic acid bacteria. The baking recipe and flour composition indicated that barley and millet bread belonged to the staple food already in the first millennium BC and suggested the role of Turpan basin as a major route for cultural communication between Western and Eastern Eurasia in antiquity. This article is part of a Special Issue entitled: Proteomics of non-model organisms. BIOLOGICAL SIGNIFICANCE We demonstrate that organic residues of thousand year old foods unearthed by archeological excavations can be analyzed by geLC-MS/MS proteomics with good representation of protein source organisms and coverage of sequences of identified proteins. In-depth look into the foods proteome identifies the food type and its individual ingredients, reveals ancient food processing technologies, projects their social and economic impact and provides evidence of intercultural communication between ancient populations. Proteomics analysis of ancient organic residues is direct, quantitative and informative and therefore has the potential to develop into a valuable, generally applicable tool in archaeometry. This article is part of a Special Issue entitled: Proteomics of non-model organisms.

Archaeobotanical Study of Ancient Food and Cereal Remains at the Astana Cemeteries, Xinjiang, China

Starch grain, phytolith and cereal bran fragments were analyzed in order to identify the food remains including cakes, dumplings, as well as porridge unearthed at the Astana Cemeteries in Turpan of Xinjiang, China. The results suggest that the cakes were made from Triticum aestivum while the dumplings were made from Triticum aestivum, along with Setaria italica. The ingredients of the porridge remains emanated from Panicum miliaceum. Moreover, direct macrobotantical evidence of the utilization of six cereal crops, such as Triticum aestivum, Hordeum vulgare var. coeleste, Panicum miliaceum, Setaria italica, Cannabis sativa, and Oryza sativa in the Turpan region during the Jin and Tang dynasties (about 3rd to 9th centuries) is also presented. All of these cereal crops not only provided food for the survival of the indigenous people, but also spiced up their daily life.

Identification of milk component in ancient food residue by proteomics.

Background Proteomic approaches based on mass spectrometry have been recently used in archaeological and art researches, generating promising results for protein identification. Little information is known about eastward spread and eastern limits of prehistoric milking in eastern Eurasia. Methodology/Principal Finding In this paper, an ancient visible food remain from Subeixi Cemeteries (cal. 500 to 300 years BC) of the Turpan Basin in Xinjiang, China, preliminarily determined containing 0.432 mg/kg cattle casein with ELISA, was analyzed by using an improved method based on liquid chromatography (LC) coupled with MALDI-TOF/TOF-MS to further identify protein origin. The specific sequence of bovine casein and the homology sequence of goat/sheep casein were identified. Conclusions/Significance The existence of milk component in ancient food implies goat/sheep and cattle milking in ancient Subeixi region, the furthest eastern location of prehistoric milking in the Old World up to date. It is envisioned that this work provides a new approach for ancient residue analysis and other archaeometry field.

Journey to the east: Diverse routes and variable flowering times for wheat and barley en route to prehistoric China

Today, farmers in many regions of eastern Asia sow their barley grains in the spring and harvest them in the autumn of the same year (spring barley). However, when it was first domesticated in southwest Asia, barley was grown between the autumn and subsequent spring (winter barley), to complete their life cycles before the summer drought. The question of when the eastern barley shifted from the original winter habit to flexible growing schedules is of significance in terms of understanding its spread. This article investigates when barley cultivation dispersed from southwest Asia to regions of eastern Asia and how the eastern spring barley evolved in this context. We report 70 new radiocarbon measurements obtained directly from barley grains recovered from archaeological sites in eastern Eurasia. Our results indicate that the eastern dispersals of wheat and barley were distinct in both space and time. We infer that barley had been cultivated in a range of markedly contrasting environments by the second millennium BC. In this context, we consider the distribution of known haplotypes of a flowering-time gene in barley, Ppd-H1, and infer that the distributions of those haplotypes may reflect the early dispersal of barley. These patterns of dispersal resonate with the second and first millennia BC textual records documenting sowing and harvesting times for barley in central/eastern China.

Archaeobotanical evidence of plant utilization in the ancient Turpan of Xinjiang, China: a case study at the Shengjindian cemetery

The Shengjindian cemeteries, Xinjiang, China, were discovered during the construction of a road in 2007, following which a salvage archaeological excavation was performed. Systemic archaeobotanical studies were applied to the plant remains, which were radiocarbon dated to 2,400–2,000 bp (about 500 bc–1 bc/ad). Altogether 33 taxa were identified. Five species of cereal remains were discovered, viz. Triticum aestivum, Panicum miliaceum, Hordeum vulgare, H. vulgare var. coeleste, as well as Setaria italica. The extent of the finds suggested that all of them were cultivated locally at that time. There were 38 fragments of grape seeds discovered within three tombs, which suggested occasional fruit cultivation by the indigenous people. Wild plants like reed and bulrush were also utilized by the ancient people in mat and thread making. The seed of Capparis spinosa may have been utilized for its medicinal and/or oil value. All of the above suggested that plants played important roles in the daily life of the ancient people buried in the Shengjindian cemetery.

Pollen and phytolith evidence for rice cultivation and vegetation change during the mid-late holocene at the Jiangli site, Suzhou, East China.

Pollen and phytolith analyses were undertaken at the Jiangli site in Suzhou, Jiangsu Province, combined with studies on macrofossils by flotation. The concentration of pollen decreased while the percentage of Poaceae pollen in the profile increased from the late phase of the Majiabang Culture to the Songze Culture suggesting that human impact on the local environment intensified gradually. The discovery of rice paddy implies a relatively advanced rice cultivation in this area during the middle-late Holocene. Other than phytoliths, the high percentage of Oryza-type Poaceae pollen (larger than 40 µm) supplied robust evidence for the existence of rice paddy. Moreover, the fact that the farther from the rice paddy, the lower the concentration and percentage of Poaceae pollen also proves that the dispersal and deposition of pollen is inversely proportional to the distance.

Pollen and Phytoliths from Fired Ancient Potsherds as Potential Indicators for Deciphering Past Vegetation and Climate in Turpan, Xinjiang, NW China

It is demonstrated that palynomorphs can occur in fired ancient potsherds when the firing temperature was under 350°C. Pollen and phytoliths recovered from incompletely fired and fully fired potsherds (ca. 2700 yrs BP) from the Yanghai Tombs, Turpan, Xinjiang, NW China can be used as potential indicators for reconstructing past vegetation and corresponding climate in the area. The results show a higher rate of recovery of pollen and phytoliths from incompletely fired potsherds than from fully fired ones. Charred phytoliths recovered from both fully fired and incompletely fired potsherds prove that degree and condition of firing result in a permanent change in phytolith color. The palynological data, together with previous data of macrobotanical remains from the Yanghai Tombs, suggest that temperate vegetation and arid climatic conditions dominated in the area ca. 2700 yrs BP.

Identification of Cannabis Fiber from the Astana Cemeteries, Xinjiang, China, with Reference to Its Unique Decorative Utilization

Identification ofCannabisFiber from the Astana Cemeteries, Xinjiang, China, with Reference to Its Unique Decorative Utilization: In the Turpan District of Xinjiang, China, large numbers of ancient clay figurines, with representations including equestrians, animals, and actors, have been excavated from the Astana Cemeteries and date from about the 3rd to the 9th centuries C.E. Based on visual inspection, the tails of some of the figurines representing horses are made of plant fibers. Fourier Transform Infrared (FTIR) spectroscopy, light microscope examination, and drying–twist tests demonstrated that these fibers were extracted from one or more stalks of hemp (Cannabis) plants. This is a unique report of the utilization of Cannabis bast fibers for figurine decoration in ancient Turpan.阿斯塔那古墓群出土大麻纤维的鉴定分析及其装饰功能的探讨. 中国新疆吐鲁番地区阿斯塔那古墓群出土了大量年代为公元3–9世纪的泥俑,其中包括骑马俑,动物俑和演员俑等。肉眼观察发现骑马俑的马尾可能由植物纤维制成。通过傅里叶变换红外光谱、光学显微镜观察和纤维旋转方向实验鉴定出这些植物纤维来自于大麻纤维。这一发现为我们提供了吐鲁番先民利用大麻纤维装饰泥俑的新证据。

Diverse lifestyles and populations in the Xiaohe culture of the Lop Nur region, Xinjiang, China

The archaeological culture found in Xiaohe Cemetery (1980–1450 BC) is one of the early Bronze Age cultures in Xinjiang, northwestern China. The material assemblages from Xiaohe culture display features with both eastern and western influences. These east-west cultural and dietary interactions may be observed via the diet of the Xiaohe population. This paper examined the stable carbon and nitrogen isotopes of human and animal bones and human hairs from the Xiaohe Cemetery and compared with those of human bones from the Gumugou Cemetery, another Xiaohe culture site. The results indicate that the diets of the peoples from the Xiaohe culture varied significantly over different periods. The unified diets of the earlier periods reflect that an admixed population first settled in the Lop Nur region and primarily engaged in animal husbandry. In the later periods, the transformations in the human diets in this region reflect that new immigrants constantly relocated here and promoted population complexity over time. Moreover, this population occasionally produced small quantities of domesticated wheat and millet. The complex population and diversified economy of the Xiaohe culture were due to the expansion of the coeval cultures in the Eurasian steppe and eastern immigrants. Additionally, the millet cereal was probably mainly used for ritual practices rather than for staple food in the later periods.