Hongxing Niu

Simple metabolic modelling of vero cell growth on glucose in fixed-bed bioreactors

Abstract A novel metabolic model is formulated to simulate Vero cell growth on glucose in fixed-bed bioreactors by simplification of glucose fluxes in the central carbon metabolism. Model formulation is based on three designated metabolism states: respiratory metabolism, overflow metabolism and critical metabolism. The model is validated by experimental culture results and successfully predicts the dynamics of cell growth, glucose consumption and lactate production. As well as quantitatively describing glucose overflow metabolism in mammalian cell culture, the proposed model will be helpful for directly monitoring and controlling metabolism state of cells in order to achieve process optimization.

Macroscopic modelling of overflow metabolism in fed-batch cultures of hybridoma cells

Abstract A macroscopic model that takes into account phenomena of overflow metabolism within glycolysis and glutaminolysis is proposed to simulate hybridoma HB-58 cell cultures. The model of central carbon metabolism is reduced to a set of macroscopic reactions. The macroscopic model describes three metabolism states: respiratory metabolism, overflow metabolism and critical metabolism. It is validated with experimental data of fed-batch hybridoma cultures and successfully predicts the dynamics of cell growth and death, substrate consumption (glutamine and glucose) and metabolites production (lactate and ammonia). Model parameters and confidence intervals are obtained via a non linear least squares identification. This model, on the one hand, allows quantitatively describing overflow metabolism in mammalian cell cultures and, one the other hand, will be valuable for monitoring and control of fed-batch cultures in order to optimize the process.