Houda Benrahma
Pasteur Institute
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Publication
Featured researches published by Houda Benrahma.
PLOS ONE | 2012
Abdelmajid Eloualid; Omar Abidi; Majida Charif; Brahim El Houate; Houda Benrahma; Noureddine Louanjli; Elbakkay Chadli; Maria Ajjemami; Abdelhamid Barakat; Anu Bashamboo; Ken McElreavey; Houria Rhaissi; Hassan Rouba
The methylenetetrahydrofolate reductase (MTHFR) gene is one of the main regulatory enzymes involved in folate metabolism, DNA synthesis and remethylation reactions. The influence of MTHFR variants on male infertility is not completely understood. The objective of this study was to analyze the distribution of the MTHFR C677T and A1298C variants using PCR-Restriction Fragment Length Polymorphism (RFLP) in a case group consisting of 344 men with unexplained reduced sperm counts compared to 617 ancestry-matched fertile or normozoospermic controls. The Chi square test was used to analyze the genotype distributions of MTHFR polymorphisms. Our data indicated a lack of association of the C677T variant with infertility. However, the homozygous (C/C) A1298C polymorphism of the MTHFR gene was present at a statistically high significance in severe oligozoospermia group compared with controls (OR = 3.372, 95% confidence interval CI = 1.27–8.238; p = 0.01431). The genotype distribution of the A1298C variants showed significant deviation from the expected Hardy-Weinberg equilibrium, suggesting that purifying selection may be acting on the 1298CC genotype. Further studies are necessary to determine the influence of the environment, especially the consumption of diet folate on sperm counts of men with different MTHFR variants.
Genetic Testing and Molecular Biomarkers | 2012
Houda Benrahma; Omar Abidi; Leila Melouk; Maria Ajjemami; Hassan Rouba; Asmaa Chadli; Mounia Oudghiri; Ahmed Farouqui; Abdelhamid Barakat
AIMS Type 2 diabetes mellitus (T2DM) is a major public health problem around the world. The C677T and A1298C polymorphisms of the methylenetetrahydrofolate reductase (MTHFR) gene have been reported to be associated with T2DM and its complications. This study aimed to investigate this association in the Moroccan population. METHODS A case-control study was performed among 282 Moroccan diabetic patients and 232 healthy controls. The MTHFR C677T and A1298C polymorphisms were genotyped by polymerase chain reaction, followed by enzymatic digestion with HinfI and MboII enzymes, respectively. RESULTS There was a significant association between C677T polymorphism and T2DM in both additive and dominant models. In addition, the 677T allele frequency differed significantly between the diabetic and control groups (26.06% vs. 33.20%, respectively). However, no significant association was found between A1298C polymorphism and T2DM. The frequencies of combined genotypes 677CC/1298AA and 677CT/1298AC differed significantly between the diabetic and control groups (32.62% vs. 20.61% and 9.57% vs. 17.55%, respectively). CONCLUSIONS These results show an evident association between the MTHFR C677T polymorphism and T2DM in Moroccan patients but no significant association with the MTHFR A1298C polymorphism.
Journal of Diabetes | 2015
Khaled Lasram; Nizar Ben Halim; Houda Benrahma; Sounnia Mediene-Benchekor; Imen Arfa; Sana Hsouna; Rym Kefi; Henda Jamoussi; Slim Ben Ammar; Sonia Bahri; Abdelmajid Abid; Soraya Benhamamouch; Abdelhamid Barakat; Sonia Abdelhak
The insulin‐like growth factor 2 mRNA‐binding protein 2 (IGF2BP2) and the cyclin‐dependent kinase 5 regulatory subunit‐associated protein 1‐like 1 (CDKAL1) identified through genome‐wide association (GWA) studies have been shown to be associated with Type 2 diabetes in various ethnic groups. In this study, we investigated the association of the rs7756992 of CDKAL1 and the rs4402960 of IGF2BP2 with Type 2 diabetes, diabetic complications (nephropathy, retinopathy and cardiovascular disease), obesity and hypertension in a Tunisian population.
International Journal of Pediatric Otorhinolaryngology | 2010
Halima Nahili; Majida Charif; Redouane Boulouiz; Safaa Bounaceur; Houda Benrahma; Omar Abidi; Abdelaziz Chafik; Hassan Rouba; Mostafa Kandil; Abdelhamid Barakat
UNLABELLED Mutations in mitochondrial DNA (mtDNA), especially the A1555G transition in the 12S rRNA gene, are one of the causes of both aminoglycoside-induced and non-syndromic sensorineural hearing loss. OBJECTIVE The aim of this study was to determine the prevalence of the A1555G mitochondrial mutation in Moroccan patients. METHODS We performed molecular characterization by PCR-RFLP and direct sequencing of one hundred and sixty four patients (84 unrelated familial and 80 sporadic cases) with a congenital sensorineural non-syndromic hearing loss and one hundred normal hearing controls for the occurrence of the A1555G mutation. RESULTS Mutational analysis of the mtDNA showed the presence of the homoplasmic A1555G mutation in three families, leading to a frequency of 3.6% similar to that reported for European-populations. No A1555G mutation was detected in sporadic and controls cases. However, we detected in twenty normal hearing controls a novel polymorphism A1557C, which was not found in patient samples. We further evidenced the presence of the A1438G mitochondrial polymorphism in four patients with sensorineural hearing loss and in five controls. CONCLUSION Our results show that the occurrence of the A1555G mutation in hearing impaired patients accounts for 3.6% in a Moroccan patients and those novel mtDNA polymorphisms might contribute to a novel sub-haplogroup specific of the Magrheb.
BioMed Research International | 2014
Khaled Lasram; Nizar Ben Halim; Sana Hsouna; Rym Kefi; Imen Arfa; Welid Ghazouani; Henda Jamoussi; Houda Benrahma; Najla Kharrat; Ahmed Rebai; Slim Ben Ammar; Sonia Bahri; Abdelhamid Barakat; Abdelmajid Abid; Sonia Abdelhak
Aims. Genetic association studies have reported the E23K variant of KCNJ11 gene to be associated with Type 2 diabetes. In Arab populations, only four studies have investigated the role of this variant. We aimed to replicate and validate the association between the E23K variant and Type 2 diabetes in Tunisian and Arab populations. Methods. We have performed a case-control association study including 250 Tunisian patients with Type 2 diabetes and 267 controls. Allelic association has also been evaluated by 2 meta-analyses including all population-based studies among Tunisians and Arabs (2 and 5 populations, resp.). Results. A significant association between the E23K variant and Type 2 diabetes was found (OR = 1.6, 95% CI = 1.14–2.27, and P = 0.007). Furthermore, our meta-analysis has confirmed the significant role of the E23K variant in susceptibility of Type 2 diabetes in Tunisian and Arab populations (OR = 1.29, 95% CI = 1.15–1.46, and P < 10−3 and OR = 1.33, 95% CI = 1.13–1.56, and P = 0.001, resp.). Conclusion. Both case-control and meta-analyses results revealed the significant association between the E23K variant of KCNJ11 and Type 2 diabetes among Tunisians and Arabs.
Pathologie Biologie | 2015
F. Lakbakbi el Yaagoubi; Hicham Charoute; A. Bakhchane; M. Ajjemami; Houda Benrahma; A. Errouagui; M. Kandil; Hassan Rouba; Abdelhamid Barakat
The aim of the present study is to explore the association between the APOA5 polymorphisms and haplotypes with obesity in Moroccan patients. The study was performed in 459 subjects, Obese (n=164) and non-obese (n=295). All subjects were genotyped for the APOA5 -1131T>C (rs662799) and c.56C>G (rs3135506) polymorphisms. The contribution of APOA5 polymorphisms and haplotypes in the increased risk of obesity were explored using logistic regression analyses. The -1131T>C and c.56C>G polymorphisms were significantly associated with obesity. Both polymorphisms were strongly associated with increased BMI. Analysis of constructed haplotypes showed a significant association between CG haplotype and susceptibility to obesity (OR [95%CI]=3.09 [1.93-4.97]; P<0.001). These results support a potential role for APOA5 common variants and related haplotypes as risk factors for obesity.
Human Mutation | 2015
Hicham Charoute; Amina Bakhchane; Houda Benrahma; Lilia Romdhane; Khalid Gabi; Hassan Rouba; Malika Fakiri; Sonia Abdelhak; Guy Lenaers; Abdelhamid Barakat
The Mediterranean basin has been the theater of migration crossroads followed by settlement of several societies and cultures in prehistoric and historical times, with important consequences on genetic and genomic determinisms. Here, we present the Mediterranean Founder Mutation Database (MFMD), established to offer web‐based access to founder mutation information in the Mediterranean population. Mutation data were collected from the literature and other online resources and systematically reviewed and assembled into this database. The information provided for each founder mutation includes DNA change, amino‐acid change, mutation type and mutation effect, as well as mutation frequency and coalescence time when available. Currently, the database contains 383 founder mutations found in 210 genes related to 219 diseases. We believe that MFMD will help scientists and physicians to design more rapid and less expensive genetic diagnostic tests. Moreover, the coalescence time of founder mutations gives an overview about the migration history of the Mediterranean population. MFMD can be publicly accessed from http://mfmd.pasteur.ma.
Mitochondrial DNA | 2018
Hicham Charoute; Rym Kefi; Safaa Bounaceur; Houda Benrahma; Ahmed Reguig; Mostafa Kandil; Hassan Rouba; Amina Bakhchane; Sonia Abdelhak; Abdelhamid Barakat
Abstract In this study, we investigated the association of mtDNA variants and haplogroups with Type 2 diabetes (T2D) in Moroccan patients. The Hypervariable Segments 1 of the mtDNA was sequenced in 108 diabetic patients and 97 controls. Association analyses were performed using Fisher’s exact test and multivariate logistic regression. The prevalence of five mtDNA variants (C16187T, C16270T, T16172C, A16293G, and C16320T) was significantly higher in cases than in controls. Among these variants, only C16270T (p = .02) and C16320T (p = .03) remains significant after adjusting by age and gender. We showed that C16270T and C16320T variants were strongly associated with increased risk of T2D in Moroccan patients.
Indian Journal of Human Genetics | 2013
Majida Charif; Redouane Boulouiz; Amina Bakhechane; Houda Benrahma; Halima Nahili; Abdelmajid Eloualid; Hassan Rouba; Mostafa Kandil; Omar Abidi; Guy Lenaers; Abdelhamid Barakat
BACKGROUND: Hearing loss is the most prevalent human genetic sensorineural defect. Mutations in the CLDN14 gene, encoding the tight junction claudin 14 protein expressed in the inner ear, have been shown to cause non-syndromic recessive hearing loss DFNB29. AIM: We describe a Moroccan SF7 family with non-syndromic hearing loss. We performed linkage analysis in this family and sequencing to identify the mutation causing deafness. MATERIALS AND METHODS: Genetic linkage analysis, suggested the involvement of CLDN14 and KCNE1 gene in deafness in this family. Mutation screening was performed using direct sequencing of the CLDN14 and KCNE1 coding exon gene. RESULTS: Our results show the presence of c.11C>T mutation in the CLDN14 gene. Transmission analysis of this mutation in the family showed that the three affected individuals are homozygous, whereas parents and three healthy individuals are heterozygous. This mutation induces a substitution of threonine to methionine at position 4. CONCLUSION: These data show that CLDN14 gene can be i mplicated in the development of hearing loss in SF7 family; however, the pathogenicity of c.11C>T mutation remains to be determined.
Journal of Diabetes | 2015
Khaled Lasram; Nizar Ben Halim; Houda Benrahma; Sounnia Mediene-Benchekor; Imen Arfa; Sana Hsouna; Rym Kefi; Henda Jamoussi; Slim Ben Ammar; Sonia Bahri; Abdelmajid Abid; Soraya Benhamamouch; Abdelhamid Barakat; Sonia Abdelhak
The insulin‐like growth factor 2 mRNA‐binding protein 2 (IGF2BP2) and the cyclin‐dependent kinase 5 regulatory subunit‐associated protein 1‐like 1 (CDKAL1) identified through genome‐wide association (GWA) studies have been shown to be associated with Type 2 diabetes in various ethnic groups. In this study, we investigated the association of the rs7756992 of CDKAL1 and the rs4402960 of IGF2BP2 with Type 2 diabetes, diabetic complications (nephropathy, retinopathy and cardiovascular disease), obesity and hypertension in a Tunisian population.