Howard M. Etlinger

Humoral factors important in resistance of salmonid fish to bacterial disease. I. Serum antibody protection of rainbow trout (Salmo gairdneri) against vibriosis

Abstract Serum antibody is shown by passive immunization and absorption experiments to be an important component of defense mechanisms against experimentally induced vibriosis in rainbow trout ( Salmo gairdneri ). Antigen from heat-killed Vibrio anguillarum injected into trout with Freunds complete adjuvant induced much higher agglutinin titers than formalin-killed bacteria with or without adjuvant, or heat-killed bacteria without adjuvant. Passively transferred trout anti- Vibrio anguillarum serum provided protection against experimental vibriosis in trout for more than 2 months. Passively transferred anti- Vibrio anguillarum rabbit serum also protected trout.

Evolution of the lymphoid system. III. Morphological and functional consequences of mitogenic stimulation of rainbow trout lymphocytes.

Abstract The morphological and functional consequences of mitogenic stimulation of rainbow trout (RBT) lymphocytes were examined. Both lipopolysaccharide from gram negative bacteria (LPS) and purified protein derivative from tuberculin (PPD) were shown to be capable of inducing the in vitro formation of plasma cells. In addition, each mitogen induced polyclonal antibody production in cultured cells, suggesting that LPS and PPD stimulate RBT B cells.

Rainbow trout leukocyte culture: a simplified method.

SummaryThe addition of 10% fetal bovine serum to Leibovitz’s L-15 culture medium resulted in marked growth of peripheral blood leukocytes from rainbow trout,Salmo gairdneri. Culture medium without serum or with 20% homologous serum did not induce substantial growth. In contrast to what has been reported by others, oxygenation of the culture medium was found not to be required for excellent cell growth.