Hua YuPing

A review of sturgeon virosis

Sturgeon, as a kind of rare large-scale economic fish, has high scientific research and commercial value. But virosis was a significant cause of mortality among farm-raised juvenile sturgeon, which brought tremendous loss in the last decades. In this paper, we reviewed the studies on four sturgeon viruses: white sturgeon (Acipenser transmontanus) iridovirus (WSIV), white sturgeon herpesvirus-1,2 (WSHV-1,2) and shovelnose sturgeon (Scaphirhynchus Scaphirhynchus) iridovirus (SSIV). The content of this review were mainly focused on clinic symptom of infected fish, diagnostic method, virus isolation, prophylactic and remedy. Based on a detailed conclusion and analysis, it is reasonable that molecular biology techniques might be a potential method for sturgeon virosis diagnosis.

Assembling a physical map of the genome by marker sequences.

Molecular genetic maps were commonly constructed by analyzing the segregation of restriction fragment length polymorphisms (RFLPs). Here we described methodology-marker sequences in a new mapping based on recent documents. With the methods they were unique sequences detected by the polymerase chain reaction (PCR). Each of the methods had its limitations and the current trend was to integrate the maps produced by the different methods. Market sequences contained mainly expressed sequence tags (ESTs), polymorphic sequence-tagged sites (STSs), randomly amplified polymorphic DNA (RAPDs), cleaved amplified polymorphic sequences (CAPS), amplified fragment length polymorphism (AFLPs), genome sequence sampling (GSS) and sequence-tagged connectors (STCs) in this paper.

Molecular cloning and analysis of the partial sequence ofRhinopithecus roxellanae growth hormone gene

Growth hormone gene (GH) ofRhinopithecus roxellanae was amplified by PCR based on the sequences of the reported mammalian growth hormone gene for the first time. The amplified fragment was about 1.8 kb. It was cloned and its upper stream was sequenced. This sequencing region consists of a 5′flanking regulatory region, exon I and part of exon II, intron I of growth hormone gene. Comparing the corresponding sequences of growth hormone gene betweenRhinopithecus roxellanae and the porcine, we concluded that the homology reached 81% in the region, and there was high conservation in the 5′flanking sequence. The kinds of amino acids of exon I and exon II for about 90% were the same to those in pig. Many mutations occurred in the degenerate site of the triplet code. In the nucleotides of intron I, there were only 72% homologies with those in pig. It means that introns and 3′flanking sequence maybe play an important part in growth hormone gene regulation of the different animals.