Hung-Goo Hwang

Identification of Quantitative Trait Loci Associated with Rice Eating Quality Traits Using a Population of Recombinant Inbred Lines Derived from a Cross between Two Temperate japonica Cultivars

Improved eating quality is a major breeding target in japonica rice due to market demand. In this study, we performed genetic analysis to identify quantitative trait loci (QTLs) that control rice eating quality traits using 192 recombinant inbred lines (RILs) derived from a cross between two japonica cultivars, ‘Suweon365’ and ‘Chucheongbyeo’. We evaluated the stickiness (ST) and overall evaluation (OE) of cooked rice using a sensory test, the glossiness of cooked rice (GCR) using a Toyo-taste meter, and measured the amylose content (AC), protein content (PC), alkali digestion value (ADV), and days to heading (DH) of the RILs in the years 2006 and 2007. Our analysis revealed 21 QTLs on chromosomes 1, 4, 6, 7, 8, and 11. QTLs on chromosomes 6, 7, and 8 were detected for three traits related to eating quality in both years. QTLs for ST and OE were identified by a sensory test in the same region of the QTLs for AC, PC, ADV, GCR and DH on chromosome 8. QTL effects on the GCR were verified using QTL-NILs (near-isogenic lines) of BC3F4–6 in the Suweon365 background, a low eating quality variety, and some BC1F3 lines. Chucheongbyeo alleles at QTLs on chromosomes 7 and 8 increased the GCR in the NILs and backcrossed lines. The QTLs identified by our analysis will be applicable to future marker-assisted selection (MAS) strategies for improving the eating quality of japonica rice.

Resistance Genes and Their Effects to Blast in Korean Rice Varieties (Oryza sativa L.)

A total of 98 varieties consisting of 88 japonica and 10 Tongil-type of rice were analyzed to resistant genes and their effects to blast. The 13 major blast resistance (R) genes against Magnaporthe oryzae were screened in a number of Korean rice varieties using molecular markers. Twenty-eight (28.6%) were found to contain the Pia gene originating from Japanese japonica rice genotypes. The Pib gene from BL1 and BL7 was incorporated into 39 Korean japonica varieties, whereas this same gene from the IRRI-bred indica varieties was detected in all Tongil-type variety. The Pii gene was found in 17 of the japonica varieties. The Pii gene in Korean rice varieties originates from the Korean japonica variety Nongbaeg, and Japanese japonica varieties Hitomebore, Inabawase, and Todorokiwase. The Pi5 gene, which clusters with Pii on chromosome 9, was identified only in an Tongil-type, Taebaeg. Thirty-four varieties were found to contain alleles of the resistance gene Pita or Pia-2. The Pita gene in japonica varieties was inherited from the Japanese japonica genotype Shimokita, and the Pita-2 gene was from Fuji280 and Sadominori. Seventeen japonica and one Tongil-type varieties contained the Piz gene, which in the japonica varieties originates from Fukuhikari and 54BC-68. The Piz-t gene contained in three Tongil-type varieties was derived from IRRI-bred indica rice varieties. The Pi9(t) gene locus that is present in Korean japonica and Tongil-type varieties was not inherited from the original Pi9 gene from wild rice Oryza minuta. The Pik-multiple allele genes Pik, Pik-m, and Pik-p were identified in 24 of the varieties tested. The Pit gene inherited from the indica rice K59 was not found in any of the Korean rice varieties tested. In haplotype analysis for the loci related to two QTLs as well as five major resistant genes and, two

A high biomass yield and whole crop silage rice cultivar 'Mogyang'.

R

Fine mapping of a grain weight quantitative trait locus on rice chromosome 8 using near-isogenic lines derived from a cross between Oryza sativa and Oryza rufipogon

genes Piz and Pita showed stable resistant effects to blast nurseries and isolates in~Korea.

Fine mapping of a yield-enhancing QTL cluster associated with transgressive variation in an Oryza sativa x O. rufipogon cross

‘Mogyang’, a new high biomass yield and whole crop silage rice (Oryza sativa L.) cultivar, is developed by the rice breeding team of National Institute of Crop Science, R.D.A., Suwon, Korea, during the period from was to 2010 and released in 2011. It was derived from a cross between SR24592-HB2319/IR73165-B-6-1-1. This cultivar has about 118 days of growth duration from seeding to heading and has long and erect leaves (culm length 93 cm). It has tough thick culm with strong lodging tolerance. This cultivar has a few less tillers per hill and markedly more spikelet numbers per panicle. ‘Mogyang’ has wide and stay green leaf compared other whole crop silage varieties. This new variety has grain shattering resistance and disease resistance for blast and dwarf virus. This variety has good qualities for whole crop silage with high TDN (Total Digestive Nutrient) yield and low NDF (Neutral Detergent Fiber) and low ADF (Acid Detergent Fiber) compared to common high grain yield varieties. The biomass and TDN yield performance of ‘Mogyang’ was 59.5% (chemical properties), 17.7 MT/ha (biomass yield), individually in local adaptability test for three years. ‘Mogyang’ is adaptable to central plain area, southern plain area of Korea.