Hwan Hee Jang

Estrogen-like activity of aqueous extract from Agrimonia pilosa Ledeb. in MCF-7 cells

BackgroundPostmenopausal women experience estrogen deficiency-related menopausal symptoms (e.g., hot flashes and mood swings) and a dramatic increase in the incidence of chronic diseases. Although estrogen-replacement therapy (ERT) can reduce mortality from cardiovascular disease and improve osteoporosis and menopausal symptoms, its side effects have limited recent use. This study investigated the estrogen-like activity of aqueous extract from Agrimonia pilosa Ledeb.MethodsThe estrogenic activity of A. pilosa was investigated by using several in vitro assays. The binding activity of A. pilosa on estrogen receptors was examined using a fluorescence polarization-based competitive binding assay. The proliferative activity of A. pilosa was also examined using MCF-7 cells. Furthermore, the effect of A. pilosa on the expression of 3 estrogen-dependent genes was assessed.ResultsUsing liquid chromatography-mass spectrometry, the 3 major peaks of A. pilosa aqueous extract were identified as apigenin-hexose, luteolin-glucuronide, and apigenin-glucuronide. The aqueous extract induced the proliferation of estrogen receptor-positive MCF-7 cells (p < 0.05). A. pilosa-stimulated proliferation was blocked on adding the estrogen antagonist ICI 182,780. Moreover, A. pilosa treatment increased the mRNA expression of the estrogen-responsive genes pS2 and PR (p < 0.05).ConclusionsThese results suggest A. pilosa can be used to improve estrogen deficiency-related menopausal symptoms or to treat diseases in postmenopausal women.

In Vitro Analysis of the Immunomodulating Effects of Allium Hookeri on Lymphocytes, Macrophages, and Tumour Cells

The present study investigated the effects of ethanol extracts of Allium hookeri (leaf, root, and fermented root) on parameters of innate immunity, tumour cell viability and antioxidant effect in vitro. Innate immunity was measured by spleen lymphocyte proliferation, nitric oxide production by chicken macrophage HD11 cells and suppressive effect on tumour cell viability was assessed using chicken RP9 cells. Free radical scavenging capacity as a measure of antioxidant capacity was determined by 0.15 mM of DPPH solution. In vitro culture of chicken spleen lymphocytes with ethanol extract of Allium hookeri (62.5–500 µg/mL) significantly induced higher proliferation compared with media control. Stimulation of macrophages with ethanol extract of Allium hookeri (62.5–500 µg/mL) showed increased Nitric oxide production. Tumor cells growth was significantly inhibited by extracts of Allium hookeri at 15.6–125 µg/mL compared with medium control and all extracts exhibited greater than 80% scavenging activity at 1000 µg/mL compared with ethanol vehicle control. Above all, fermented root extracts showed strongest effects on antioxidant activity compared to leaf and root extracts.

Changes in Antioxidant and Antimicrobial Activities of Schizandra chinensis Baillon under Different Solvent Extraction

The present study was performed to investigate antioxidant and antimicrobial activities of Schizandra chinensis Baillon under different extraction conditions. Extraction yield of water extract, 70% ethanol extract, and methanol extract from S. chinensis was 17.7, 32.9, and 36.6% respectively. Total polyphenol content was 9.2-16.3 mg/g in S. chinensis extracts and was increased significantly by ethanol and methanol extraction (p < 0.05). Radical scavenging activity against DPPH and ABTS·+ was significantly higher in ethanol and methanol extracts than in water extract (p < 0.05). Ethanol and methanol extracts from S. chinensis also showed effective superoxide dismutase-like activity compared with water extract (p < 0.05). S. chinensis water extract exhibited strong antimicrobial activity against Escherichia coli, Staphylococcus aureus, and Salmonella typhimurium, which was increased significantly by ethanol and methanol extraction (p < 0.05). On the other hand, inhibitory effect of S. chinensis water extract on nitric oxide production was more effective than ethanol and methanol extract.

Estrogen-like activity of Adenophora triphylla var. japonica water extract in MCF-7 cells

This study investigated the estrogen-like activity of Adenophora triphylla var. japonica, which has been shown to have pharmacological activities. Water extracts from A. triphylla (ATWE) could bind to estrogen receptors and displaced binding of E2 to ERα and ERβ. ATWE stimulated the proliferation of estrogen receptor-positive MCF-7 cells in a dose dependent manner (p<0.05). ATWE induced proliferation was blocked by the addition of the estrogen antagonist ICI 182,780 (p<0.05). Moreover, ATWE treatment caused a significant increase in mRNA expression of estrogen-responsive genes (pS2, PR, and cathepsin D; p<0.05). These results indicate that A. triphylla has estrogen-like activity and could be used to improve estrogen deficiency-related menopausal symptoms or diseases in postmenopausal women.

Analysis of Variation in Anthocyanin Composition in Korean Coloured Potato Cultivars by LC-DAD-ESI-MS and PLS-DA

Anthocyanin concentration and composition and the effect of steaming and baking on these were evaluated in tubers of Korean red- and purple-fleshed potato cultivars and breeding clones using liquid chromatography with diode array detection and electrospray ionization-mass spectrometry (LC-DAD-ESI-MS). Twenty-six anthocyanins were isolated, of which 24 were identified. Remarkably, five cis isomers were identified, of which four, viz., cis-petanin, cis-peonanin, petunidin 3-cis-caffeoylrutinoside-5-glucoside, and petunidin 3-cis-feruloylrutinoside-5-glucoside, are reported for the first time. Moreover, pelargonidin 3-p-coumaroylrutinoside-5-glucoside (pelanin), peonidin 3-p-coumaroylrutinoside-5-glucoside (peonanin) and petunidin 3-p-coumaroylrutinoside-5-glucoside (petanin) were identified as the principal anthocyanins. We found that the total anthocyanin content of coloured potatoes was decreased by steaming and baking compared with the raw state. In addition, we performed partial least square discriminant analysis (PLS-DA) to discriminate between the analyzed anthocyanins. Cis isomers seemed to play a vital role as a biomarker in the PLS-DA model based on the type of processing and colour of the tubers.