Hye Jin Lim
Chungnam National University
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Featured researches published by Hye Jin Lim.
Biotechnology for Biofuels | 2016
Hye Jin Lim; Yu Jin Park; Yeon Jae Jang; Ji Eun Choi; Joon Young Oh; Ji Hyun Park; Jae Kwang Song; Dong‑Myung Kim
BackgroundPhospholipase A1 is an enzyme that hydrolyzes phospholipids at the sn-1 position. It has potential applications across diverse fields including food, pharmaceutical, and biofuel industries. Although there has been increasing interest in the use of phospholipase A1 for degumming of plant oils during biodiesel production, production of recombinant phospholipase A1 has been hampered by low efficiency of gene expression and its toxicity to the host cell.ResultsWhile expression of phospholipase A1 in Escherichia coli resulted in extremely low productivity associated with inhibition of transformed cell growth, drastically higher production of functional phospholipase A1 was achieved in a cell-free protein synthesis system where enzyme expression is decoupled from cell physiology. Compared with expression in E. coli, cell-free synthesis resulted in an over 1000-fold higher titer of functional phospholipase A1. Cell-free produced phospholipase A1 was also used for successfully degumming crude plant oil.ConclusionsWe demonstrate successful production of Serratia sp. phospholipase A1 in a cell-free protein synthesis system. Including the phospholipase A1 investigated in this study, many industrial enzymes can interfere with the regular physiology of cells, making cellular production of them problematic. With the experimental results presented herewith, we believe that cell-free protein synthesis will provide a viable option for rapid production of important industrial biocatalysts.
Journal of Industrial and Engineering Chemistry | 2018
Tong Yi; Hye Jin Lim; So Jeong Lee; Kyung-Ho Lee; Dong-Myung Kim
Biochemical Engineering Journal | 2018
Hye Jin Lim; Kyung-Ho Lee; Dong-Myung Kim
한국생물공학회 학술대회 | 2017
Hye Jin Lim; Yi tonG; Ye Seop Park; Tae Hyeon Yoo; Dong-Myung Kim
한국생물공학회 학술대회 | 2016
Hye Jin Lim; Jin-Ho Kwon; Dong-Myung Kim
한국생물공학회 학술대회 | 2016
Hye Jin Lim; Yu Jin Park; Yeon Jae Jang; Devi Kasi; Christy Catherine; Kyung-Ho Lee; Tong Yi; Ji Hyun Park; Joon Young Oh; So Jeong Lee; Jae Kwang Song; Dong-Myung Kim
한국생물공학회 학술대회 | 2016
Hye Jin Lim; Dong-Myung Kim
한국생물공학회 학술대회 | 2016
Devi Kasi; Hye Jin Lim; Christy Catherine; Yujin Park; Kyung-Ho Lee; Dong-Ki Choi; Yong-Sung Kim; Dong-Myung Kim
Journal of Bioscience and Bioengineering | 2016
Gyeong Tae Eom; Joon Young Oh; Ji Hyun Park; Hye Jin Lim; So Jeong Lee; Eun-Young Kim; Ji-Eun Choi; Jonggeon Jegal; Bong Keun Song; Ju-Hyun Yu; Jae Kwang Song
한국생물공학회 학술대회 | 2015
Joon Young Oh; Hee Taek Kim; Ji-Eun Choi; Eun-Young Kim; Hye Jin Lim; Jae Kwang Song