Hyun Jae Chung
Yonsei University
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Featured researches published by Hyun Jae Chung.
Gut | 2006
Jong Eun Yeon; Wangdon Yoo; Sun Pyo Hong; Yun Jung Chang; Sang Kyun Yu; Ji Hoon Kim; Yeon Seok Seo; Hyun Jae Chung; Myoung Soon Moon; Soo-Ok Kim; Kwan Soo Byun; Chang Hong Lee
Background: Adefovir dipivoxil (ADV) is a potent nucleotide analogue against both the wild-type and lamivudine (LMV) resistant hepatitis B virus (HBV). The cumulative incidence of ADV resistant mutations in the nucleoside/-tide treatment naïve chronic hepatitis B patient (CHB) at weeks 48, 96, and 144 was 0, 0.8–3%, and ∼5.9%, respectively. Aims: The aim of this study was to characterise the genotypic and phenotypic mutation profiles to ADV in 67 LMV resistant CHB patients who were treated with ADV. Methods: Serum HBV DNA was quantified by real time polymerase chain reaction. The ADV mutant was detected using matrix assisted laser desorption/ionisation time of flight mass spectrometry based genotyping assays, termed restriction fragment mass polymorphism (RFMP). Results: RFMP analysis revealed that a total of 11 amino acid substitutions developed in the rt domain of the HBV polymerase in nine patients. The cumulative incidence of genotypic ADV resistance at months 12 and 24 was 6.4% and 25.4%, respectively. The rtA181V, rtN236T, and rtA181T mutations were detected in five, four, and two of the 67 patients at treatment months 12–17, 3–19, and 7–20, respectively. Serial quantification of serum HBV DNA revealed that two patients with the rtA181V mutation, with or without the rtN236T mutation, and one patient with the rtA181T mutation displayed HBV DNA rebound. Conclusion: Emergence of the ADV mutation in LMV resistant patients who are treated with ADV appeared to present earlier and more frequently than was reported in previous studies on nucleoside/-tide treatment naïve patients.
Nature Protocols | 2008
Sun Pyo Hong; Soo-Kyung Shin; Eun Hee Lee; Eun Ok Kim; Seung Il Ji; Hyun Jae Chung; Sun Nie Park; Wangdon Yoo; William R. Folk; Soo-Ok Kim
We describe a matrix-assisted laser desorption/ionization–time of flight (MALDI-TOF) mass spectrometry (MS)-based assay for human papillomavirus (HPV) genotyping—the restriction fragment mass polymorphism (RFMP) assay, which is based on mass measurement of genotype-specific oligonucleotide fragments generated by TypeIIS restriction endonuclease cleavage after recognition sites have been introduced by PCR amplification. The use of a TypeIIS restriction enzyme makes the RFMP assay independent of sequence and applicable to a wide variety of HPV genotypes, because these enzymes have cleavage sites at a fixed distance from their recognition sites. After PCR amplification, samples are subjected to restriction enzyme digestion with FokI and BtsCI and desalting using Oasis purification plates, followed by analysis by MALDI-TOF MS. Overall, the protocol is simple, takes ∼4–4.5 h and can accurately detect and identify at least 74 different HPV genotypes.
Antiviral Therapy | 2005
Hyon Suk Kim; Kwang-Hyub Han; Sang Hoon Ahn; Eun-Ok Kim; Hye-Young Chang; Myoung Soon Moon; Hyun Jae Chung; Wangdon Yoo; Soo-Ok Kim; Sun Pyo Hong
Clinical Chemistry | 2005
Yoon Jun Kim; Soo-Ok Kim; Hyun Jae Chung; Mi Sun Jee; Byeong Gwan Kim; Kang Mo Kim; Jung-Hwan Yoon; Hyo-Suk Lee; Chung Yong Kim; Sukjoon Kim; Wangdon Yoo; Sun Pyo Hong
The Korean Journal of Hepatology | 2004
Jung Min Lee; Sang Hoon Ahn; Hye Young Chang; Ji Eun Shin; Do Young Kim; Myoung Ki Sim; Sun Pyo Hong; Hyun Jae Chung; Soo Ok Kim; Kwang Hyub Han; Chae Yoon Chon; Young Myoung Moon
The Korean Journal of Hepatology | 2005
Hye Young Chang; Sang Hoon Ahn; Do Young Kim; Jeon Soo Shin; Yong Soo Kim; Sun Pyo Hong; Hyun Jae Chung; Soo Ok Kim; Wang Don Yoo; Kwang Hyub Han
Korean Journal of Laboratory Medicine | 2007
Eun Hee Lee; Hyun Jae Chung; Heung Bum Oh; Hyun Sook Chi; Mi Sun Jee; Sun Nie Park; Sun Pyo Hong; Wangdon Yoo; Soo-Ok Kim
Archive | 2017
Soo Ok Kim; 김수옥; Suk Joon Kim; 김석준; Sun Pyo Hong; 홍선표; Hyun Jae Chung; 정현재; Woo Jae Cho; 조우재; Jae Il Kim; 김재일; Seung Min Yang; 양승민; Ae Ri Cho; 조애리; Seong Soo Hong; 홍성수; Jeong Woo Kim; 김정우; Sun Young Jeong; 정선영
Archive | 2017
Soo Ok Kim; 김수옥; Suk Joon Kim; 김석준; Sun Pyo Hong; 홍선표; Hyun Jae Chung; 정현재; Woo Jae Cho; 조우재; Jae Il Kim; 김재일; Seung Min Yang; 양승민; Ae Ri Cho; 조애리; Seong Soo Hong; 홍성수; Jeong Woo Kim; 김정우; Sun Young Jeong; 정선영
Archive | 2017
Soo Ok Kim; 김수옥; Suk Joon Kim; 김석준; Sun Pyo Hong; 홍선표; Hyun Jae Chung; 정현재; Woo Jae Cho; 조우재; Jae Il Kim; 김재일; Seung Min Yang; 양승민; Ae Ri Cho; 조애리; Seong Soo Hong; 홍성수; Jeong Woo Kim; 김정우; Sun Young Jeong; 정선영