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Dive into the research topics where Hyun Jae Chung is active.

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Featured researches published by Hyun Jae Chung.


Gut | 2006

Resistance to adefovir dipivoxil in lamivudine resistant chronic hepatitis B patients treated with adefovir dipivoxil

Jong Eun Yeon; Wangdon Yoo; Sun Pyo Hong; Yun Jung Chang; Sang Kyun Yu; Ji Hoon Kim; Yeon Seok Seo; Hyun Jae Chung; Myoung Soon Moon; Soo-Ok Kim; Kwan Soo Byun; Chang Hong Lee

Background: Adefovir dipivoxil (ADV) is a potent nucleotide analogue against both the wild-type and lamivudine (LMV) resistant hepatitis B virus (HBV). The cumulative incidence of ADV resistant mutations in the nucleoside/-tide treatment naïve chronic hepatitis B patient (CHB) at weeks 48, 96, and 144 was 0, 0.8–3%, and ∼5.9%, respectively. Aims: The aim of this study was to characterise the genotypic and phenotypic mutation profiles to ADV in 67 LMV resistant CHB patients who were treated with ADV. Methods: Serum HBV DNA was quantified by real time polymerase chain reaction. The ADV mutant was detected using matrix assisted laser desorption/ionisation time of flight mass spectrometry based genotyping assays, termed restriction fragment mass polymorphism (RFMP). Results: RFMP analysis revealed that a total of 11 amino acid substitutions developed in the rt domain of the HBV polymerase in nine patients. The cumulative incidence of genotypic ADV resistance at months 12 and 24 was 6.4% and 25.4%, respectively. The rtA181V, rtN236T, and rtA181T mutations were detected in five, four, and two of the 67 patients at treatment months 12–17, 3–19, and 7–20, respectively. Serial quantification of serum HBV DNA revealed that two patients with the rtA181V mutation, with or without the rtN236T mutation, and one patient with the rtA181T mutation displayed HBV DNA rebound. Conclusion: Emergence of the ADV mutation in LMV resistant patients who are treated with ADV appeared to present earlier and more frequently than was reported in previous studies on nucleoside/-tide treatment naïve patients.


Nature Protocols | 2008

High-resolution human papillomavirus genotyping by MALDI-TOF mass spectrometry

Sun Pyo Hong; Soo-Kyung Shin; Eun Hee Lee; Eun Ok Kim; Seung Il Ji; Hyun Jae Chung; Sun Nie Park; Wangdon Yoo; William R. Folk; Soo-Ok Kim

We describe a matrix-assisted laser desorption/ionization–time of flight (MALDI-TOF) mass spectrometry (MS)-based assay for human papillomavirus (HPV) genotyping—the restriction fragment mass polymorphism (RFMP) assay, which is based on mass measurement of genotype-specific oligonucleotide fragments generated by TypeIIS restriction endonuclease cleavage after recognition sites have been introduced by PCR amplification. The use of a TypeIIS restriction enzyme makes the RFMP assay independent of sequence and applicable to a wide variety of HPV genotypes, because these enzymes have cleavage sites at a fixed distance from their recognition sites. After PCR amplification, samples are subjected to restriction enzyme digestion with FokI and BtsCI and desalting using Oasis purification plates, followed by analysis by MALDI-TOF MS. Overall, the protocol is simple, takes ∼4–4.5 h and can accurately detect and identify at least 74 different HPV genotypes.


Antiviral Therapy | 2005

Evaluation of methods for monitoring drug resistance in chronic hepatitis B patients during lamivudine therapy based on mass spectrometry and reverse hybridization

Hyon Suk Kim; Kwang-Hyub Han; Sang Hoon Ahn; Eun-Ok Kim; Hye-Young Chang; Myoung Soon Moon; Hyun Jae Chung; Wangdon Yoo; Soo-Ok Kim; Sun Pyo Hong


Clinical Chemistry | 2005

Population Genotyping of Hepatitis C Virus by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Analysis of Short DNA Fragments

Yoon Jun Kim; Soo-Ok Kim; Hyun Jae Chung; Mi Sun Jee; Byeong Gwan Kim; Kang Mo Kim; Jung-Hwan Yoon; Hyo-Suk Lee; Chung Yong Kim; Sukjoon Kim; Wangdon Yoo; Sun Pyo Hong


The Korean Journal of Hepatology | 2004

[Reappraisal of HBV genotypes and clinical significance in Koreans using MALDI-TOF mass spectrometry].

Jung Min Lee; Sang Hoon Ahn; Hye Young Chang; Ji Eun Shin; Do Young Kim; Myoung Ki Sim; Sun Pyo Hong; Hyun Jae Chung; Soo Ok Kim; Kwang Hyub Han; Chae Yoon Chon; Young Myoung Moon


The Korean Journal of Hepatology | 2005

Association between CCR5 promoter polymorphisms and hepatitis B virus infection

Hye Young Chang; Sang Hoon Ahn; Do Young Kim; Jeon Soo Shin; Yong Soo Kim; Sun Pyo Hong; Hyun Jae Chung; Soo Ok Kim; Wang Don Yoo; Kwang Hyub Han


Korean Journal of Laboratory Medicine | 2007

[Human papilloma virus genotyping assay using restriction fragment mass polymorphism analysis, and its comparison with sequencing and hybrid capture assays].

Eun Hee Lee; Hyun Jae Chung; Heung Bum Oh; Hyun Sook Chi; Mi Sun Jee; Sun Nie Park; Sun Pyo Hong; Wangdon Yoo; Soo-Ok Kim


Archive | 2017

절단된 상보적인 태그 절편을 이용한 표적 핵산 서열 검출 방법 및 그 조성물

Soo Ok Kim; 김수옥; Suk Joon Kim; 김석준; Sun Pyo Hong; 홍선표; Hyun Jae Chung; 정현재; Woo Jae Cho; 조우재; Jae Il Kim; 김재일; Seung Min Yang; 양승민; Ae Ri Cho; 조애리; Seong Soo Hong; 홍성수; Jeong Woo Kim; 김정우; Sun Young Jeong; 정선영


Archive | 2017

METHOD FOR DETECTING TARGET NUCLEIC ACID SEQUENCE USING CLEAVED COMPLEMENTARY TAG FRAGMENT AND COMPOSITION THEREOF

Soo Ok Kim; 김수옥; Suk Joon Kim; 김석준; Sun Pyo Hong; 홍선표; Hyun Jae Chung; 정현재; Woo Jae Cho; 조우재; Jae Il Kim; 김재일; Seung Min Yang; 양승민; Ae Ri Cho; 조애리; Seong Soo Hong; 홍성수; Jeong Woo Kim; 김정우; Sun Young Jeong; 정선영


Archive | 2017

PROCÉDÉ DE DÉTECTION DE SÉQUENCE D'ACIDE NUCLÉIQUE CIBLE À L'AIDE D'UN FRAGMENT DE MARQUEUR COMPLÉMENTAIRE CLIVÉ ET COMPOSITION ASSOCIÉE

Soo Ok Kim; 김수옥; Suk Joon Kim; 김석준; Sun Pyo Hong; 홍선표; Hyun Jae Chung; 정현재; Woo Jae Cho; 조우재; Jae Il Kim; 김재일; Seung Min Yang; 양승민; Ae Ri Cho; 조애리; Seong Soo Hong; 홍성수; Jeong Woo Kim; 김정우; Sun Young Jeong; 정선영

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Sun Pyo Hong

Seoul National University

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Soo-Ok Kim

Seoul National University

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Wangdon Yoo

Seoul National University

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Sun Nie Park

Food and Drug Administration

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