Hyun-Ok Yang

Apoptosis-inducing effect of diketopiperazine disulfides produced by Aspergillus sp. KMD 901 isolated from marine sediment on HCT116 colon cancer cell lines.

Aims:  Research is to identify the bioactive secondary metabolites produced by Aspergillus sp. KMD 901 isolated from marine sediment and to assess their apoptosis‐inducing effects.

Autophagy inducing Effect of modified Yeoldahanso-tang and its related Proteins in SH-SY5Y cells

Abstract Autophagy inducing Effect of modified Yeoldahanso-tang and its related Proteins in SH-SY5Y cells Hee-Ju Kim, Na-Young Bae ∗ , Moon-Hee Jang † , Hyun-Ok Yang, Taek-Won Ahn † Natural Medicine Center, Korea Institute of Science and Technology ∗ Dept. of Sasang Constitution Medicine, Pusan National University School of Korean Medicine † Dept. of Sasang Constitution Medicine, College of Korean Medicine, Daejeon UnivObjectivesModified Yeolda-Hanso tang (MYH) is a traditional herbal formula in Korea for various diseases. MYH is containingthe 10 herbs : Pueraria lobata (Willd.) Ohwi, Angelica tenuissima Nakai, Scutellaria baicalensis Georgi, Platycodon grandiflorum (Jacq), Angelicae Dahurica , Cimicifuga heracleifolia Kom, Raphanus sativa L., Polygala tenuifolia (Willd), Acorus gramineus Soland and Dimocarpus longan Lour. The 10 herbs is constituted as a ratio of the 6:4:2:1:2:2:2:4:6:6. We investigatedneuroprotective effects of MYH on human neuroblastoma SH-SY5Y cells and evaluated the ability of MYH to prevent and treat for neurodegenerative diseases such as Parkinson’s disease via basal autophagy enhancement.MethodsPharmacological induction of Autophagy by MYH in SH-SY5Y cells:Induction of autophagy by MYH in human neuroblastoma SH-SY5Y cells was carreid out by immunoblot analysiswith several autophagy markers. SH-SY5Y cells were treated with MYH at the concentration of 400 and 800 μg/ml for 24 hr. Specifically, the autophagosome proteins LC3 II and Atg5 levels were increased and autophagy pathway related proteins such as beclin-1, PI3 Kinase class III protein, ULK1, mTOR and AMPK were activated.ConclusionsMYH can enhance the induction of autophagy through key regulator AMPK, mTOR, and Beclin-1 and it shouldbe considered as a possible candidate of neuroprotective agents for such as Parkinson’s disease.Key Words: Modified Yeolda-Hanso tang (MYH), Neuroprotection, Parkinson’s disease, Autophagy