I. C. S. Lima

IN VITRO EVALUATION OF GOAT CAUDA EPIDIDYMAL SPERM, COOLED IN DIFFERENT EXTENDERS AT 4 C°

portuguesA colheita de espermatozoides da cauda do epididimo (CES) e uma opcao viavel de preservacao de material genetico de especies ameacadas de extincao, bem como para uso em reproducao assistida. Este trabalho teve como objetivo verifi- car a eficiencia de quatro diferentes diluidores sobre os espermatozoides da cauda do epididimo de caprinos, resfriado a 4 oC. Os espermatozoides epididimarios foram recuperados da cauda de seis pares de epididimo. As amostras de cada par foram misturadas e subdivididas em quatro aliquotas que foram diluidas na agua de coco- gema (CW-EY), solucao fisiologica glicosada 0,5 %-gema (PSG), leite desnatado UHT (ME) e leite desnatado UHT-gema (ME-EY). As amostras foram resfriadas a 4 °C por 2, 12, 24 e 48 horas. Apos o armazenamento, em cada periodo, o CES foi avaliado atraves do teste de termoresistencia quanto a motilidade, vigor espermatico e alteracoes morfologicas total. A taxa de degradacao da motilidade foi calculada no final de cada periodo. A viabilidade espermatica diminuiu com o tempo de refrigeracao nos quatro diluidores. Os CES diluidos na CW-EY apresentaram maiores valores para vigor e motilidade, embora estes parâmetros nao deferissem daqueles mantidos no PSG por ate 48 horas, exceto para vigor espermatico. Entretanto, os diluidores PSG e ME ocasionaram maiores danos morfologicos aos CES a partir de 12 e 48 horas, respectivamente. Os diluidores ME e ME- EY proporcionaram a celula espermatica menor estabilidade durante 48 h de conservacao. EnglishCollection of spermatozoa obtained from the epididymis cauda (CES) is a viable option to pre- serve of genetic material from threatened species and for use in assisted reproduction. The aim of the present study was to assess the in vitro effect of four different extenders on spermatozoa from goat epididymis cauda, cooled at 4 oC. Epididymal sperm were recovered from the cauda by flushing six pairs of epididymis. The samples of each pair were mixed, subdivided into four aliquots and diluted in coconut water-egg yolk (CW-EY), physiologic solution with 0.5 % glucose and egg yolk (PSG-EY), UHT skim milk (SM), and UHT skim milk with egg yolk (SM-EY). The samples were cooled at 4 oC for 2, 12, 24 and 48 hours. After storage, in each period, the semen was evaluated by the heat resistance test for sperm motility, vigor and total morphological alterations. The motility degradation rate was calculated at the end of each time period. Sperm viability decreased with time in the four extenders. Epididymal sperm diluted in CW-EY showed higher values for vigor and motility, although these parameters did not differ from the sperm kept in the PSG-EY up to 48 hours at 4 °C, except for spermatic vigor. However, the PSG-EY and SM extenders caused greater morphologic damage to epididymal sperm after 12 and 24 hours, respectively. Extenders based on skim milk provided less spermatic cell stability during 48 hours. In conclusion, CW-EY extender was the most efficient extender to maintain CES viability at 4 oC.

Biometry testicle- epididymidis and crioula ovine reserve of epididymal sperm

Biometria Testículo-Epididimária e a Reserva Espermática Epididimária de Ovinos Sem Padrão Racial Definido Biometry testicleepididymidis and crioula ovine reserve of epididymal sperm Alexandre Weick Uchoa Monteiro 1 ; Ítalo Cordeiro Silva Lima 2 ; Igo Renan Albuquerque de Andrade 3 ; Gabrimar Araújo Martins 3 ; Airton Alencar Araújo 5 ; Ana Claudia Silva Dias 6 ; Francisca Nivanda de Lima Estevam 7 , Gyselle Viana Aguiar 8 ; Ana Cláudia Nascimento Campos 9 ________________________________________________________________________________________________________

Effect of initial seminal plasma fructose concentration on goat semen storage at 5ºC

EnglishTwenty-four goat semen samples were collected and divided into four aliquots, diluted with the citrate-egg yolk (CY), TRIS-egg yolk (TY) or industrialized coconut water with egg yolk (ICW-Y) extenders. The fourth aliquot was centrifuged to analyze fructose concentration and PLA 2 activity on SP. The semen was stored at 5 o C and evaluated at times fresh, 2, 24 and 48 h, in each time was evaluated the vigor, sperm motility and total morphological alterations. The animals were divided into two groups: group I- fructose concentration >710 mg/dL and group II- fructose concentration portuguesForam coletadas 24 amostras de semen capri- no. Cada ejaculado foi dividido em 4 aliquotas, e foram diluidas em citrato-gema de ovo (CG), TRIS- gema de ovo (TG) e agua de coco industrializada- gema de ovo (ACI-G), a quarta, foi centrifugada para determinacao da concentracao de frutose e atividade da FLA 2 no PS. O semen foi conservado a 5 oC e avaliado a fresco, 2, 24 e 48 h, em cada tempo foi avaliado o vigor, motilidade e alteracoes morfologicas. Os reprodutores foram divididos em dois grupos: grupo I-concentracao de frutose >710 mg/dL e o grupo II-concentracao de frutose Nenhuma diferenca na atividade da FLA 2 foi en- contrada entre os grupos. Por outro lado, a ACI- G nao deve ser utilizada para a conservacao do semen caprino.