I. D. Smith

Protocol Guided Bleeding Management Improves Cardiac Surgery Patient Outcomes

Excessive bleeding is a risk associated with cardiac surgery. Treatment invariably requires transfusion of blood products; however, the transfusion itself may contribute to postoperative sequelae. Our objective was to analyse a quality initiative designed to provide an evidenced‐based approach to bleeding management.

An animal model for human melanoma.

Abstract— Experimental animal models that are directly relevant to human melanoma are lacking. We propose the Angora goat as a potentially useful field model with experimental potential and to this end have examined the prevalence and site distribution of all skin cancers in 28 Angora goat herds in Queensland, Australia. The prevalence of benign melanocytic lesions (lentigines) and their experimental induction by sunlight were also investigated. Among 1731 goats over 2 years of age, 139 malignant skin tumors were excised from 95 affected animals. The prevalence of squamous cell carcinoma (SCC) was 3.8% and of melanoma, 2.2%. Main site of occurrence of melanoma (83%) was the dorsal surface of the ear; in contrast SCC occurred mostly (84%) on the perineum. Lentigines were darker and more prevalent on the exposed compared with the unexposed surface of the ear in Angoras, analogous to the higher prevalence of nevi on the exposed compared with the less exposed inner surface of the arm in humans. Lentigines, which were also found on the perineum though lighter in color than on the dorsal ear, were absent in young animals under 3 months but were numerous in 1–3 year olds. Furthermore in an experimental substudy eight goats, having one flank repeatedly shorn and the contralateral flank left unshorn, revealed consistently more solar lentigines on the shorn flank (P < 0.05) when both sides were examined after 9 months. Histopathological examination of paired skin biopsies from five of these goats also showed more abundant pigmentation in skin from the exposed, as compared with the unexposed flank. These findings indicate that sunlight induces tumors and lentigines in goats in a highly site‐specific manner. The Angora goat model may suggest paradigms for explaining the site differences observed for human melanoma and may also be useful in the future clarification of molecular changes following carcinogenic levels of sun exposure.

Squeezing information from regional climate change projections - results from a synthesis of CMIP5 results for south-east Queensland, Australia

We present a synthesis of CMIP5 model results for projected rainfall changes for a single region (south-east Queensland, Australia) and note that, as was evident in CMIP3 results, the multi-model mean projected changes for the late 21st century are not statistically significant for any season nor annually. Taking account of the number of statistically significant changes to mean rainfall, we find some evidence favouring a decrease in both spring and annual rainfall, but this is not compelling. In almost all cases the most frequent result is for no significant change. However, if we consider the number of results where there is a statistically significant change in the distributions of rainfall amounts, there appears to be slightly more information available for risk assessment studies. These numbers suggest an increase in the frequency of both wet and dry events during summer and spring, and a shift towards more frequent dry events during winter. There is no evidence for any significant changes to the distributions for either autumn or annually. The findings suggest that, in one respect, multi-model rainfall projections may contain more information than is evident from syntheses which focus on changes to the means and that, for some regions where changes in the frequency of wet and dry seasons/years have known impacts, the model projections may be more valuable than previously thought.

The Neurochemical Pathology of Thiamine Deficiency : GABAA and GlutamateNNMDA Receptor Binding Sites in a Goat Model

Synaptic plasma membranes were prepared from four cerebrocortical areas from six male Angora goats made chronically thiamine deficient (TD) by the administration of AmproliumTM (600–900 mg/kg daily for 38–44 d) .Four male controls were matched for age (27–30 mo). Four different radioligands were used to characterise GABAA and Glu-RNMDA receptor binding sites. There were marked, localised and contrasting changes in motor cortex, with an increase in GABAA and a decrease in Glu-RNMDA binding site densities. Less clearcut changes of a similar nature were seen in visual cortex. There was no variation in the parameters of GABGABA-activated [3H]diazepam binding between cortical areas in control goats, but there was a reduction in the maximal response to GABA in all areas in TD goats . There were regional variations in glutamate-activated [3H]MK-801 binding in control goat brain, and a non-selectively reduced maximal response in TD. Alterations in these indices of GABA- and glutamate-mediated neurotransmission may underlie the neurological signs of acute thiamine deficiency in these animals.