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Featured researches published by I. M. Ray.


Journal of Chemical Ecology | 1996

Variation in aphid alarm pheromone content among glandular and eglandular-hairedMedicago accessions.

Reza Mostafavi; John A. Henning; J. Gardea-Torresday; I. M. Ray

Pea (Acyrthosiphon pisum Harris) and blue alfalfa aphid (A. kondoi Shinji) deterrency in alfalfa (Medicago saliva L.) may result from incorporating higher levels of the aphid alarm pheromone,(E)-β-farnesene relative to(E)-β-caryophyllene. We evaluated five eglandular and two glandular-haired alfalfa accessions for differences in(E)-β-farnesene and(E)-β-caryophyllene content under glasshouse conditions using supercritical fluid extraction and gas chromatography. In addition, pea and blue alfalfa aphid olfactory behavioral tests were conducted uponMedicago species containing different ratios of(E)-β-famesene relative to(E)-β-caryophyllene. No differences in(E)-β-caryophyllene content were observed among the seven entries (μ=0.42 ng/g plant material). Significant differences (P ≤ 0.05) among entries were observed for(E)-β-famesene content, with KS94GH6 exhibiting the highest (1.18 ng/g), and CUF 101 the lowest levels (0.35 ng/g). Elite tetraploid sources possessed significantly lower levels (μ=0.42 ng/g) of(E)-β-farnesene than did wild and cultivated diploid accessions (μ=0.83 ng/g). Olfactory behavioral tests for both the pea and blue alfalfa aphids demonstrated KS94GH6 repelled aphids while cultivated alfalfa types attracted aphids in each case. Previously demonstrated aphid resistance in diploid KS94GH6 may result from superior(E)-β- farnesene levels, but(E)-β-farnesene is probably not a factor in cultivated alfalfa resistance. Finally, accession KS94GH6 could act as an excellent germplasm resource for the incorporation of higher(E)-β-farnesene levels into cultivated alfalfa.


Archive | 2004

EST-SSRs for Genetic Mapping in Alfalfa

Mary K. Sledge; I. M. Ray; M. A. Rouf Mian

Several genetic maps of alfalfa have been published, both at the diploid (Medicago sativa subsp. coerulea) and tetraploid (Medicago sativa L.) levels. These maps have been constructed primarily with RFLP and RAPD markers, and have not been integrated into a single, reference map. The development of reliable, publicly available PCR-based markers would provide a basis for unifying the variously available alfalfa genetic maps. The goal of the present research is to construct a molecular map of tetraploid alfalfa using EST-derived SSR markers. Two alfalfa backcross populations have been constructed from a cross between a water-use efficient, M. falcata genotype and a low water-use efficient M. sativa genotype of Chilean origin. These populations are also segregating for yield, fall dormancy, and winter hardiness. We have screened 333 primer pairs amplifying M. truncatula EST-SSRs, approximately 50% of which are polymorphic between the Chilean and M. falcata parents. We have currently identified a total of 216 single-dose alleles from 125 polymorphic EST-SSRs, and are constructing an EST-SSR map that will be used for identifying QTLs for drought tolerance in autotetraploid alfalfa.


Theoretical and Applied Genetics | 2005

An expressed sequence tag SSR map of tetraploid alfalfa (Medicago sativa L.).

M. K. Sledge; I. M. Ray; G. Jiang


Theoretical and Applied Genetics | 2004

Population-based diallel analyses among nine historically recognized alfalfa germplasms

A. Segovia-Lerma; Leigh W. Murray; M. S. Townsend; I. M. Ray


Crop Science | 1998

Variation for yield, water-use efficiency, and canopy morphology among nine alfalfa germplasms

I. M. Ray; M. S. Townsend; John A. Henning


Crop Science | 1999

Heritabilities of water-use efficiency traits and correlations with agronomic traits in water-stressed alfalfa

I. M. Ray; M. Shaun Townsend; Cherokee M. Muncy; John A. Henning


Crop Science | 1998

Evaluation of Simple Methods for Estimating Broad-Sense Heritability in Stands of Randomly Planted Genotypes

S. E. Smith; R. O. Kuehl; I. M. Ray; R. Hui; Daniela Soleri


Crop Science | 2003

Clarification and Reevaluation of Population-Based Diallel Analyses

Leigh W. Murray; I. M. Ray; Haiying Dong; Armando Segovia-Lerma


Crop Science | 1997

Carbon isotope discrimination, ash, and canopy temperature in three wheatgrass species

A. B. Frank; I. M. Ray; John D. Berdahl; James F. Karn


Plant Science | 2004

Genetic engineering ruminal stable high methionine protein in the foliage of alfalfa

Suman Bagga; Angela Armendaris; Nina Klypina; I. M. Ray; Soumitra Ghoshroy; Marcia Endress; Dennis W. Sutton; John D. Kemp; Champa Sengupta-Gopalan

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John A. Henning

Agricultural Research Service

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M. S. Townsend

New Mexico State University

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C. A. Pierce

New Mexico State University

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Leigh W. Murray

New Mexico State University

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John D. Berdahl

Agricultural Research Service

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B. A. Melton

New Mexico State University

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Juvencio González García

Autonomous University of Chihuahua

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Sergio Guerrero Morales

Autonomous University of Chihuahua

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