İbrahim Demirkan

Isolation and identification of Treponema pedis and Treponema phagedenis-like organisms from bovine digital dermatitis lesions found in dairy cattle in Turkey

The isolation and identification of microorganisms associated with digital dermatitis (DD) in Turkish dairy cattle was investigated using punch skin biopsy samples from typical clinical lesions; they were collected from dairy farms and abattoirs in 5 different geographical locations in Turkey. Morphological characteristics and flagellation types were examined using a transmission electron microscope, and their enzyme profiles by enzyme activation kits; their catalase reaction characteristics were evaluated by the addition of 3% H2O2. Their phylogenies were identified using 16S rRNA and the results compared with known gene bank data. Bacterial cells were 5.0 to 18.2 µm long, 0.2 to 0.5 µm wide, and their minimum number of periplasmic flagellum was 4 (4:8:4) with a maximum of 8 (8:16:8). All isolates were catalase negative. Of the spirochetes isolated and identified, group I organisms showed close similarity with Treponema pedis (99% genetic homology), whereas those in group II were similar to Treponema phagedenis (98% homology). This is the first report of specific sub-groups of Treponema spp., isolated from Turkish dairy cattle presenting with DD lesions, being associated with this disease; these morphotypes were similar to those found globally in housed dairy cattle units and are probably significant microorganisms associated with the aetiopathogensis of this infectious disease causing acute bovine lameness. These results suggest that the distribution of DD-associated treponemes is not specific to particular geographic regions of Turkey.

Kedi Gingivitis Sağaltımında Amoksisilin – Klavulanik Asit Ve Sulfadimetilprimidin – Trimetoprim Ajanlarının Klinik Etkilerinin Karşılaştırılması

Bu calismada kedilerde teshis edilen ginigivitis olgularinin amoksisilin ve trimetoprim ile karsilastirmali olarak tedavi edilme secenekleri arastirildi. Bu amacla, klinige agiz problemi sikayeti ile getirilen toplam 22 adet kedi yas, cinsiyet ve irk farki gozetilmeksizin iki gruba ayrildi. Gruplardaki bireylerin hastaliklarinin siddeti yapilan klinik muayene bulgularina gore gingival indekse paralel bir sekilde belirlendi. Birinci gruptaki hastalara amoksisilin-klavulanik asit (amoksisilin 7 mg + klavulanik asit 1.75 mg, deri alti, kg/canli agirlik) ihtiva eden preparatlar uygulandi. Ayni sekilde, ikinci gruptaki hastalara rimetoprim-sulfanamid (trimethoprim 4 mg + sulfadimetilpirimidin 20 mg, deri alti, kg/canli agirlik) ihtiva eden preparatlar uygulandi. Yapilan degerlendirme sonucunda, hastaligin iyilesme suresi acisindan, iki ilac grubu arasinda istatistiksel olarak onemli sayilabilecek bir farklilik gozlenmedi. Bu sonucun, kucuk hayvan klinigi yapan veteriner hekimler icin degerli bir veri oldugu dusunulmektedir.