Igor Kostenyuk
University of Florida
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Publication
Featured researches published by Igor Kostenyuk.
Journal of Agricultural and Food Chemistry | 2013
Jinhe Bai; Elizabeth A. Baldwin; Hui-Ling Liao; Wei Zhao; Igor Kostenyuk; Jacqueline K. Burns; Mike Irey
Orange juice processed from Huanglongbing (HLB) affected fruit is often associated with bitter taste and/or off-flavor. HLB disease in Florida is associated with Candidatus Liberibacter asiaticus (CLas), a phloem-limited bacterium. The current standard to confirm CLas for citrus trees is to take samples from midribs of leaves, which are rich in phloem tissues, and use a quantitative real-time polymerase chain reaction (qPCR) test to detect the 16S rDNA gene of CLas. It is extremely difficult to detect CLas in orange juice because of the low CLas population, high sugar and pectin concentration, low pH, and possible existence of an inhibitor to DNA amplification. The objective of this research was to improve extraction of DNA from orange juice and detection of CLas by qPCR. Homogenization using a sonicator increased DNA yield by 86% in comparison to mortar and pestle extraction. It is difficult to separate DNA from pectin; however, DNA was successfully extracted by treating the juice with pectinase. Application of an elution column successfully removed the unidentified inhibitor to DNA amplification. This work provided a protocol to extract DNA from whole orange juice and detect CLas in HLB-affected fruit.
Postharvest Biology and Technology | 2011
Jinhe Bai; Elizabeth A. Baldwin; Yoshihiro Imahori; Igor Kostenyuk; Jacqueline K. Burns; Jeffrey K. Brecht
Journal of The American Society for Horticultural Science | 2004
Luis Pozo; Rongcai Yuan; Igor Kostenyuk; Fernando Alférez; Guang Yan Zhong; Jacqueline K. Burns
Journal of Experimental Botany | 2005
Rongcai Yuan; Zhencai Wu; Igor Kostenyuk; Jacqueline K. Burns
Physiologia Plantarum | 2004
Igor Kostenyuk; Jacqueline K. Burns
Hortscience | 2005
Rongcai Yuan; Fernando Alférez; Igor Kostenyuk; Shila Singh; James P. Syvertsen; Jacqueline K. Burns
Physiologia Plantarum | 2002
Igor Kostenyuk; Jerzy Zoń; Jacqueline K. Burns
Journal of Phytopathology | 2011
Marta I. Francis; Igor Kostenyuk; Vladimir Orbović; Anatoly Y. Loskutov; Mikhail A. Zolotukhin; James H. Graham
Proceedings of the Florida State Horticultural Society | 2012
Jinhe Bai; Elizabeth A. Baldwin; Hui-Ling Liao; Igor Kostenyuk; Jacqueline K. Burns; Mike Irey
Hortscience | 2011
Timothy M. Spann; Luis Pozo; Igor Kostenyuk; Jacqueline K. Burns