Ika Puspita Sari

Immunomodulatory Effects of Ethanolic Extract of Thyphonium flagelliforme (Lodd) Blume in Rats Induced by Cyclophosphamide

The present study aimed to examine the immunomodulatory effect of ethanolic extract of Typhonium flagelliforme (Lodd) Blume in cyclophosphamide-treated rats. The immunomodulatory effects were determined by lymphocytes proliferation, phagocytic activity of macrophages, plasma cytokines of tumor necrosis factor-α, interleukin-1α, interleukin-10 levels, and killer T cells (CD8+ T cells) counts. The results showed that the administration of ethanolic extract of T flagelliforme reduced immunosupessive effect on lymphocyte proliferation, increase the number and phagocytic activity of macrophages in cyclophosphamide-treated rats. Moreover, the ethanolic extract of T flagelliforme also significantly (P < .05) improved the immune system activities especially the proliferation of CD8+T cells and reduced the suppressive effects on cytokines such as tumor necrosis factor-α and interleukin-1α. In conclusion, the ethanolic extract of T flagelliforme has immunomodulatory properties in cyclophosphamide-treated rats. The results suggest that T flagelliforme can reduce immunosuppresive effect caused by a chemotherapeutic agent.

LACTAGOGUE EFFECT OF UYUP-UYUP (TRADITIONAL MEDICINE) AND Sauropus androgynus Merr EXTRACT ON PIGEON INGLUVIES GLANDS

Sauropus androgynus Merr. and uyup-uyup have been used as lactagogue traditional medicine since long time ago, but the pharmacological mechanism was unclear. This research has been done to determine prolactin induction of S. androgynus Merr and uyup-uyup as lactagogue agents. S. androgynus Merr leaf extract and uyup-uyup were given to the pigeon for 7 and 14 consecutive days, the epithelium tunica mucous of ingluvies glands induced by prolactin was then determine. The result showed that S. androgynus Merr. leaf extract and uyup-uyup have lactagogue effect as determined by prolactin induction. The induction of prolactin after S. androgynus Merr leaf extract administration (dose 54 mg/kg Body Weight (BW) for 14 days comparable to that of moloco B12 (dose 189 mg/kg BW) after 7 days administration (p<0.05). Key words : lactagogue, prolactin, tunica mucous