Ilona Nakoinz

Lymphosarcoma cell growth is selectively inhibited by B lymphocyte mitogens: LPS, dextran sulfate and PPD

Summary A series of murine lymphosarcomas, including those induced by Abelson leukemia virus, were adapted to culture. Their cell growth was inhibited by B lymphocyte mitogens at concentrations which did not affect other hematopoietic cell lines: myelomas, T lymphomas, mastocytoma and erythroleukemia. The pattern of inhibition among different lymphosarcoma lines suggests that they represent tumors of B lymphocytes at different stages of differentiation.

Independence of θ and TL surface antigens and killing by thymidine, cortisol, phytohemagglutinin, and cyclic AMP in a murine lymphoma

Summary Thymic like lymphomas bear θ antigen and often TL antigen, and are killed by low concentrations of corticosteroids. These lymphomas are also very sensitive to killing by phytohemagglutinin and thymidine, in contrast to myelomas. Since θ and TL antigens and sensitivity to steroids and phytohemagglutinin change during the maturation of thymus cells to immunocompetent lymphocytes, these properties were studied in lymphoma variants. Variants selected for loss of detectable θ antigen or sensitivity to hydrocortisone, phytohemagglutinin, thymidine, thioguanine, or cyclic AMP still retain the unselected markers. This suggests that, although thymocyte properties appear to be coordinately regulated during differentiation, the expression of each may be altered by a separate mechanism.