Imbi Truumees

Structure of Sch 68631: A new hepatitis C virus proteinase inhibitor from Streptomyces sp.

Abstract A novel hepatitis C virus (HCV) proteinase inhibitor, Sch 68631 ( 1 ), was isolated from the fermentation culture broth of Streptomyces sp. The structure of 1 was elucidated by analyses of spectroscopic data and shown to be a new member of the phenanthrenequinone family of compounds.

A novel class of antitumor metabolites from the fungus Nattrassia mangiferae

Abstract Sch 49210, Sch 53514 and Sch 53516 have been isolated from a fungal culture, and identified by analysis of 2D NMR data. Two diacetylation derivatives Sch 53515 and Sch 53517 have been synthesized. These compounds demonstrate inhibitory activity of phospholipase D (PLD), and display potent activity in the antitumor invasion chamber assay.

Two novel diketopiperazines isolated from the fungus Tolypocladium sp.

Abstract Diketopiperazines, Sch 54794 and Sch 54796, have been isolated from a fungal fermentation. The structures of these compounds have been established based on spectroscopic data analysis. Sch 54794 exhibited inhibitory activity in the platelet activating factor (PAF) assay.

A new fungal metabolite, Sch 202596, with inhibitory activity in the galanin receptor GALR1 assay

Abstract A novel spirocoumaranone, Sch 202596 ( 1 ), was isolated from the fermentation broth of Aspergillus sp. The isolation, structure elucidation and stereochemistry of 1 are described.

Sch 65676: A novel fungal metabolite with the inhibitory activity against the cytomegalovirus protease

A new secondary metabolite, Sch 65676 (1), was isolated from the fermentation broth of a fungal culture. The structure of 1 was elucidated based on comprehensive NMR studies including COSY, NOE and HMBC experiments. Compound 1 displays inhibitory activity against the cytomegalovirus (CMV) protease.

A new potent antifungal agent from Actinoplanes sp

Abstract A novel antifungal agent, Sch 56036 ( 1 ), was isolated from the fermentation culture broth of an Actinoplanes sp. Structure elucidation of 1 was accomplished by the analysis of spectroscopic data. Compound 1 was identified as a new polycyclic xanthone. Biological evaluation of 1 indicated that the compound possesses broad spectrum antifungal activity against various yeasts and dermatophytes with geometric mean MIC values ranging from 0.017∼0.031 μg/mL, respectively.

Two new phospholipase d inhibitors, sch 49211 and sch 49212, produced by the fungus nattrasia mangirferae

Abstract Sch 49211 ( 2 and Sch 49212 9 3 have been isolated from the fermentation of the fungus Nattrasia mangiferae. The structures of 2 and 3 were determined by analysis of NMR spectroscopic data. Both 2 and 3 exhibit inhibitory activity (IC 50 = 11 and 12 μm, respectively) in the phospholipase D (PLD) assay

Characterization of peptidyl-nucleoside antifungal antibiotics from fermentation broth

SummaryCharacterization of sinefungin related antifungal antibiotics from fermentation broth was accomplished by coupling photodiode array (PDA) detection to high performance liquid chromatography (HPLC). From the combined HPLC-PDA evaluation of broth filtrate, we detected five sinefungin related components. Fast atom bombardment (FAB) mass spectroscopic evaluations, mass-analysed ion kinetic energy spectra (MIKES) and collision activated (CA) MIKES of these components confirmed their respective identities. Our findings from the combination of HPLC photodiode array acquisition and FAB-mass spectrometry suggest we have detected the presence of a previously unreported sinefungin analogue.

Nebularine from a novelMicrobispora sp.

SummaryNebularine has now been isolated from a novelMicrobispora sp., identified as a new mesophilic species. An efficient method for the isolation of nebularine using Droplet Counter Current Chromatography is described.

High-performance preparative isolation and purification of several mycinamicins

Abstract High performance preparative chromatographic systems are described which facilitate the isolation and purification of some naturally occurring, novel mycinamicins. Facile separation and excellent recovery of these macrolide antibiotics was obtained with a C-18 radially compressed Prep 500 cartridge using methanol/trifluoroacetic acid mobile phases. Semi-preparative chromatography on a C-8 reversed-phase sorbent incorporating a volatile ammonium acetate buffer was utilized in the final purification of an enriched mixture of intermediate components. Monitoring of isolated fractions with analytical HPLC provided direct support for the effectiveness of the preparative isolation.