Ion Gruia

Statistical and fractal approaches in laser polarimetry diagnostics of the cancer prostate tissues

This work is directed to the investigation of the scope of the technique of laser polarimetry of oncological changes of the human prostate tissue under the conditions of multiple scattering, which presents a more general and real experimental clinical situation. To compare the above mentioned scope of biotissue laser polarimetry on the first stage the research of human prostate tissue in the conditions of single scattering was performed.

The antioxidant response induced by Lonicera caerulaea berry extracts in animals bearing experimental solid tumors.

Lonicera caerulea is a species of bush native to the Kamchatka Peninsula (Russian Far East) whose berries have been extensively studied due to their potential high antioxidant activity. The aim of our work was to investigate the in vivo effects of the antioxidant action of Lonicera caerulea berry extracts on the dynamics of experimentally-induced tumors. Our data showed that aqueous Lonicera caerulaea extracts reduced the tumor volume when administered continuously during the tumor growth and development stages, but augmented the tumor growth when the administration of extracts started three weeks before tumor grafting. Prolonged administration of Lonicera caerulaea berry extracts induced the antioxidant defense mechanism in the tumor tissues, while surprisingly amplifying the peripheral oxidative stress.

Spectropolarimetry of cancer change of biotissues

This work is directed to the investigation of the scope of the technique of laser polarimetry and polarization spectrometry of oncological changes of the human prostate tissue under the conditions of multiple scattering. It was shown that the third statistic moment in the intensity distribution proved to be the most sensitive to pathological changes in orientation structure. Its value in the intensity distribution of polarization image I (0 - 90) of oncologically changed tissue is 21 times higher if compared with the similar statistic parameter of the intensity distribution of the healthy tissue. The results of studies of size linear dichroism prostate gland, as healthy and affected by malignant tumor at different stages of its development was presented. Significant difference in the values of linear dichroism and its spectral dependence in the spectral range λ = 280 - 840 nm as between research facilities, and between biotissues - healthy (or affected by benign tumors) and cancer patients was shown. These results may have diagnostic value for detection and assessment of the development of cancer.

Spectropolarimetry in singular structure biotissue images for diagnostics of their pathological changes

This work is directed to the investigation of the scope of the technique of laser polarimetry of oncological changes of the human prostate tissue under the conditions of multiple scattering, which presents a more general and real experimental clinical situation. To compare the above mentioned scope of biotissue laser polarimetry on the first stage the research of human prostate tissue in the conditions of single scattering was performed. The analysis of the obtained results showed high diagnostic sensitivity of statistic moments of the 3rd and 4th orders of coordinate distributions of matrix elements of both types of biotissues to the changes of optical-geometric structure.

Spectropolarimetry in the differential diagnosis of benign and malignant ovarian tumors

The aim of this study was to use the spectrophotometry method to develop a diagnostic algorithm for blood studies and the content of douglas deepening in women with ovarian tumors. A comparative analysis of the blood of healthy women and patients with ovarian cancer revealed significantly greater optical anisotropy of the latter. Qualitative studies of polarization microscopic blood images revealed a very developed microcrystalline structure. Based on the study of blood and puncture and douglas deepening of healthy women and patients with benign and malignant tumors of the ovaries, using the method of laser polarimetry, experimentally developed and clinically tested photometric and polarization criteria indicating the presence of malignancy of the tumor.

Spectrometry techniques in diagnostics of hereditary breast cancer

The aim was to study the possibility of using polarimetry methods of performance evaluation of blood plasma of patients with breast cancer and spectroscopy method in the diagnosis of breast cancer and determine the criteria for their use of non-invasive screening for problems.

Combining polarimetry and spectropolarimetry techniques in diagnostics of cancer changes in biological tissues

The aim of the study is combining polarimetry and spectropolarimetry techniques for identifying the changes of opticalgeometrical structure in different kinds of biotissues with solid tumours. It is researched that a linear dichroism appears in biotissues (human esophagus, muscle tissue of rats, human prostate tissue, cervical smear) with cancer diseases, magnitude of which depends on the type of the tissue and on the time of cancer process development.

Optical pre-clinical diagnostics of the cervical tissues malignant changing

This work is directed to the investigation of the scope of the technique of laser polarimetry of oncological changes of the human prostate and cervical tissues under the conditions of multiple scattering, which presents a more general and real experimental clinical situation. This study is combining polarimetry and spectropolarimetry techniques for identifying the changes of optical-geometrical structure in different kinds of biotissues with solid tumours. It is researched that a linear dichroism appears in biotissues (human esophagus, muscle tissue of rats, human prostate tissue, cervical smear) with cancer diseases, magnitude of which depends on the type of the tissue and on the time of cancer process development.

Information optics approach in diagnostics of malignant changes of biological tissues

The information optics techniques, such as polarimetry and spectropolarimetry, are applied for identifying the changes of optical-geometrical structure in various biological tissues with solid tumours. It is shown that a linear dichroism appears in biological tissues (human esophagus, muscle tissue of rats, human prostate tissue, and cervical smear) with cancer diseases whose magnitude depends on the type of tissue and on the time of cancer process development.

Simulation of propagation of radiation in epithelial tissues

Different statistical modeling techniques of radiation propagation in epithelial tissue are considered. The two main approaches are: modified classical Monte Carlo method for light propagation in turbid medium and the coherent inverse ray tracing method. Classical Monte Carlo method was modified to take into account polarization of propagated radiation and birefringence which can occur due to tissues commonly found under epithelium. As a supplementary method modified version of classical ray tracing technique is used which takes into account phase of radiation during its propagation in tissue.