A. G. Ushenko

Wavelet analysis of two-dimensional birefringence images of architectonics in biotissues for diagnosing pathological changes

A method for polarization filtering, correlation processing, and wavelet analysis of coherent images of physiologically normal and necrotically changed (myocardium infarct) muscle tissue is presented. A technique for early optical diagnosis of the appearance of these biological tissues and the course of their degenerative-dystrophic changes is proposed.

Polarization singularities of the object field of skin surface

The paper deals with the investigation of formation mechanisms of laser radiation polarization structure scattered by an optically thin surface layer of human skin in two registration zones: a boundary field and a far zone of Fraunhofer diffraction. The conditions of forming polarization singularities by such an object in the scattered radiation field have been defined. Statistical and fractal polarization structure of object fields of physiologically normal and pathologically changed skin has been studied. It has been shown that polarization singularities of radiation scattered by physiologically normal skin samples have a fractal coordinate structure. It is characteristic for fields of pathologically changed skin to have a statistical coordinate structure of polarization singularities in all diffraction zones.

Statistical and fractal approaches in laser polarimetry diagnostics of the cancer prostate tissues

This work is directed to the investigation of the scope of the technique of laser polarimetry of oncological changes of the human prostate tissue under the conditions of multiple scattering, which presents a more general and real experimental clinical situation. To compare the above mentioned scope of biotissue laser polarimetry on the first stage the research of human prostate tissue in the conditions of single scattering was performed.

Spectropolarimetry of cancer change of biotissues

This work is directed to the investigation of the scope of the technique of laser polarimetry and polarization spectrometry of oncological changes of the human prostate tissue under the conditions of multiple scattering. It was shown that the third statistic moment in the intensity distribution proved to be the most sensitive to pathological changes in orientation structure. Its value in the intensity distribution of polarization image I (0 - 90) of oncologically changed tissue is 21 times higher if compared with the similar statistic parameter of the intensity distribution of the healthy tissue. The results of studies of size linear dichroism prostate gland, as healthy and affected by malignant tumor at different stages of its development was presented. Significant difference in the values of linear dichroism and its spectral dependence in the spectral range λ = 280 - 840 nm as between research facilities, and between biotissues - healthy (or affected by benign tumors) and cancer patients was shown. These results may have diagnostic value for detection and assessment of the development of cancer.

Spatial-frequency selection of complex degree of coherence of laser images of blood plasma in diagnostics and differentiation of pathological states of human organism of various nosology

The theoretical background of correlation and phase analysis of laser images of human blood plasma with the spatial-frequency selection of the manifestations of mechanisms of linear and circular birefringence of albumin and globulin is presented. The comparative results of measuring the coordinate distributions of the module of complex degree of coherence (CDC) of laser images of blood plasma taken from the patients of three groups--healthy patients (donors), the patients suffering from the rheumatoid arthritis, and those with stomach cancer (adenocarcinoma)--are shown. The values and ranges of change of the statistical (moments of the first-fourth orders), correlation (excess of autocorrelation functions), and fractal (slopes of approximating curves and dispersion of the extremes of logarithmic dependencies of power spectra) parameters of CDC coordinate distributions are studied. The objective criteria of diagnostics of the pathology and differentiation of the inflammation and oncological state are determined.

Correlation and fractal structure of Jones matrices of human bile secret

The interrelation of orientation, anisotropy structure of biological tissue architectonics and topological element distribution of Johns Matrices is investigated here. It is researched the analytical correlation of bioobject Johns matrices microstructure with matrix element indices measured in the far field of Fraunhofers diffraction. The investigation is also dealt with the computer modeling and experimental researching the structure of matrix operator of multifractal amorphous - crystalline organization of different morphological structure biological tissues.

Singular Structure of Polarization Images of Bile Secret in Diagnostics of Human Physiological State

There have been theoretically analyzed the ways of the formation of the polarization singularities of the biological tissues images of various morphological structures. There have been also experimentally examined the coordinate distributions of a single and doubly degenerated polarization singularities of the physiologically normal and pathologically changed biological tissues. There have been determined the statistical criteria of diagnostics of the kidney tissue collagenous disease (the 3rd and the 4th statistical moments of the linear density singularity points). It was found out that the process of the pathological change of the kidney tissue morphology leads to the formation of the self-similar (fractal) distribution of the polarization singularities of its image.

Polarization-dependent laser autofluorescence of the polycrystalline networks of blood plasma films in the task of liver pathology differentiation.

Current research presents the results of the investigation of diagnostic efficiency of laser polarization autofluorescence for the set of endogenous fluorophores of blood plasma polycrystalline films in two spectral regions (0.5-0.53 μm and 0.63-0.67 μm) under the excitation of laser radiation with a wavelength of 0.405 μm. A model of generalized optical anisotropy of protein networks of blood plasma polycrystalline films is proposed for the purpose of defining laser autofluorescence processes. Both phase (linear birefringence and optical activity) and amplitude (linear and circular dichroisms) anisotropies have been considered. Interconnections between the optimal condition of probing beam polarization state and the efficiency of induction laser autofluorescence have been found. Statistical analysis of coordinate distributions of laser polarization autofluorescence intensities is suggested by means of determination of the quantitative criteria (statistical moments of the 1st-4th orders). The efficiency of laser polarization autofluorescence of polycrystalline networks in the task of differentiation of nonalcoholic fatty liver disease and chronic hepatitis of human liver has been analyzed.

Polarization Selection Of Two-Dimensional Phase-Inhomogeneous Birefringence Images Of Biotissues

Specific features of the formation of local and statistical polarization structures of laser radiation scattered in phaseinhomogeneous layers of biological tissue were studied. The distribution of azimuth and eccentricity of boundary field polarization was found to correlate with the orientation-phase structure of phase-inhomogeneous layers. A method of polarization phase reconstruction of biological tissue architectonics was suggested.

Laser polarimetry of pathological changes in biotissues

The present paper deals with research into laser radiation polarized structure transformed by biotissue crystalline phase. This is important for developing optical methods of diagnostics of biotissue orientation and mineralized structure, as well as for modelling biocomposite materials.