Irving Young

Ultrastructure of endometrial stromal sarcoma

An endometrial stromal sarcoma from a 48‐year‐old woman was studied by light and electron microscopy. Ultrastructurally most of the tumor cells resembled the stromal cells of midproliferative endometrium. There was no evidence of a pericytic differentiation of the tumor cells. These findings lend further support to the concept of an endometrial histogenesis of stromal sarcoma.

Ultrastructural study of androgen‐producing adrenocortical adenoma

Ultrastructural study of a virilizing adrenocortical adenoma from an 18‐year‐old girl revealed tumor cells with morphological features of cells of zona reticularis. Some of the cells contained laminated membranous inclusions identical to the so‐called spironolactone bodies. These bodies have not been previously described in any of the adrenal cortical tumors. It is suggested that these bodies represent exaggerated hypertrophy of smooth endoplasmic reticulum and are thus indicative of pronounced biosynthetic activity in the tumor cells.

Elastogenesis in adenomatoid tumor. Histochemical and ultrastructural observations

An adenomatoid tumor from the round ligament of a 50‐year‐old woman was studied by light and electron microscopy. Ultrastructurally the tumor was remarkably similar to normal as well as neoplastic mesothelium, thus lending further support to the concept of a mesothelial origin of adenomatoid tumors. Comparative histochemical and ultrastructural studies revealed presence of acid mucopolysaccharides mainly along the luminal borders of the tumor cells, thus indicating likely production of this material by the tumor cells as opposed to the stromal cells. The stroma was rich in elastic fibers and there was evidence of active elastogenesis. This paper represents the first unequivocal demonstration of elastogenesis in an adenomatoid tumor.

Neurilemoma-like uterine myomas: An ultrastructural reaffirmation of their non-Schwannian nature

Some uterine leiomyomas demonstrate a curious rhythmic pattern of cellular arrangement, suggesting the appearance of nerve sheath tumors. Leiomyomas of similar appearance are more frequently found in the gastrointestinal tract. Ultrastructural examination of two such tumors from the uterus, one simulating the Antoni type A pattern and the other predominantly of the Antoni type B pattern, showed features of smooth muscle cells to be present in both. There also was a close fine structural resemblance to light microscopically similar tumors of the gastrointestinal tract. This study should dispel the implications that these tumors are of Schwannian origin.

Bronchial adenoma with polymorphous features.

A bronchial adenoma from a 35‐year‐old man presented a unique histologic appearance as it had a prominent papillary pattern and contained ciliated tumor cells. The tumor also contained areas resembling several histologically distinct subtypes of bronchial adenoma, thus indicating a close histogenetic relationship among these subtypes. The significance of cells resembling oncocytes, noted in this tumor, is discussed. It is suggested that the cells which fulfill the light microscopic criteria of oncocytes should be tentatively called oncocytoid cells, and only those cells in which mitochondrial hyperplasia can be demonstrated by electron microscopy should be designated as oncocytes. Cancer 33:1572–1576, 1974.

Mycosis fungoides-like cells: Their presence in a case of pityriasic dermatitis with a comment on their significance as an indicator of primary T-cell dyscrasia

A case of pityriasic dermatitis in which the histologic findings mimicked mycosis fungoides was examined ultrastructurally. Large numbers of mycosis-like cells were found in the dermis and within epidermal spongiotic vesicles. Such cells occur in the epidermal and dermal infiltrates of primary T-lymphocyte disorders, notably in mycosis fungoides, the Sézary syndrome, and parapsoriasis en plague. However, they have also been found in the dermal infiltrates of benign dermatoses, in some skin tumors, and occasionally in normal controls. They share ultrastructural features with transformed T lymphocytes. It is emphasized that cells with this morphology may be found in the skin in any condition involving T-cell transformation or dysplasia. The mere presence of cells with this morphology within skin infiltrates is insufficient evidence for the diagnosis of primary T-cell dyscrasia.

Detection and Localization of Antibody-Forming Cells to a Bacterial Endotoxin by an Immunobioautographic Procedure

Summary Specific antibody-forming cells to E. coli endotoxin were detected as discrete foci of bacteriolysis with intact spleen sections from mice immunized with varying concentrations of the antigen. Normal nonimmunized mice had an average of 1–2 antibody foci/spleen. Following immunization with 0.1–50 μg of endotoxin there was a rapid increase in the number of bacteriolytic foci, with a peak by the fourth to fifth day after antigen injection. Spleens of mice injected with 10–50 μg of antigen usually had confluent areas of bacteriolysis on the peak day. The animals receiving lower doses had fewer and more discrete antibody foci. Specificity was readily demonstrated by inhibition tests with specific antigen. In addition, the number of foci per spleen correlated with the increased number of single antibody plaqueforming cells, as detected by similar bacteriolytic antibody assays with dispersed cell suspensions. However, the increase in the number of single PFCs occurred later than did antibody foci. Similarly, the rise in serum antibacterial antibody, as detected by agglutination tests, occurred at a later time, with the peak usually between the fifth and eighth day after immunization. There was little or no increase in the level of serum antibody during the first 2–3 days after immunization.

The Morphologic Sites of Bacteriolytic Antibody Formation to E. Coli Antigen in Mice

The presence of immunoglobulins and antibody in cells associated with germinal centers of lymphoid follicles has been demonstrated by a number of investigators during the past few years [1, 2, 3, 4, 5, 6, 7, 8]. The distribution of antibody-containing cells in such follicles seems to follow a characteristic pattern. In addition, a number of investigators have indicated that antigen may be “trapped” in lymphoid follicles and associated germinal centers [9, 10, 11, 12, 13].