Iván Galindo

Correlation between HPV types associated with oral squamous cell carcinoma and cervicovaginal cytology: An in situ hybridization study

Epidemiologic and clinical evidence suggests a possible relationship between the human papillomavirus types found in oral squamous cell carcinoma and cervicovaginal cancer. To try to address this question, we analyzed by in situ hybridization 50 paraffin-embedded biopsies of oral squamous cell carcinomas and their corresponding Papanicolaou smears using nonradioactive DNA probes for human papillomavirus type 6/11 and 16/18. This analysis revealed that 70% (35 of 50) of oral squamous cell carcinomas and 56% (28 of 50) of Papanicolaou smears were positive for one or both of these human papillomavirus DNA probes. In 23 of the 28 patients who were positive for human papillomavirus sequences in the cervicovaginal tract, we found the same human papillomavirus type in the oral cavity. Five of the positive in situ hybridization Papanicolaou smears were associated with negative in situ hybridization oral squamous cell carcinomas. Our data suggest that after a primary infection by human papillomavirus, other mucosal sites may eventually become infected. Local environmental factors may contribute to the pathogenesis of lesions such as oral squamous cell carcinoma.

Focal epithelial hyperplasia: human-papillomavirus-induced disease with a genetic predisposition in a Venezuelan family

A study on the presence of human papillomavirus (HPV) DNA sequences and focal epithelial hyperplasia (FEH) in a family of Venezuelan ancestry has revealed that FEH is an HPV-induced disease presenting familial aggregation. The genealogical evidence indicates a genetic predisposition to the disease.

A combined polymerase chain reaction-colour development hybridization assay in a microtitre format for the detection of Clostridium spp.

We have developed a rapid and sensitive assay for the detection of clostridial cells or spores in liquid food samples. The method recognizes Clostridium 16S (small ribosomal subunit RNA) rDNA (ribosomal DNA) sequences by a polymerase chain reaction-digoxigenin-labelling protocol, coupled to a capture oligonucleotide immobilized on a microtitre plate. The positive results are revealed by means of a colour reaction. In 6 h of non-intensive labour, we can detect as few as two to five clostridial cells or spores in experimentally contaminated soft drinks.

Study of Leishmania mexicana electrokaryotype by clamped homogeneous electric field electrophoresis.

In spite of the wide use of electrokaryotypes for Leishmania identification, the number, ploidy and associated functions of the chromosomal bands still remain controversial topics. In the present work, we studied these problems in the pathogenic organism Leishmania mexicana using the clamped homogeneous electric field electrophoresis (CHEF) technique, which allows the separation of uniform chromosomal bands in one run. We arrived at the following general conclusions: (i) a comparative densitometric study using haploid Saccharomyces cerevisiae cells as standard reveals that although L. mexicana is an aneuploid organism, its larger bands are diploid; (ii) a total of 18 chromosomal bands ranging from 3.2 to 0.245 Mbp were resolved. These molecules summed to 1.34 X 10(8) bp, a value within the range of the Leishmania genome; (iii) in hybridisation experiments using different housekeeping gene probes, the majority hybridised with chromosomal band 17 or 18 of L. mexicana, with additional locations for some genes; (iv) the presence of the ubiquitous leishmanial (CA/GT)n sequence in the DNA probes could lead to erroneous gene localisation.

In situ hybridization with digoxigenin-labelled DNA probes for the detection of human papillomavirus-induced focal epithelial hyperplasia among Venezuelans

An in situ hybridization assay with digoxigenin-labelled probes was used to detect the presence of human papillomavirus (HPV) sequences in ten related Venezuelan patients with the diagnosis of focal epithelial hyperplasia. The samples displayed HPV sequences in all cases. Further restriction analysis in four of the patients suggested the presence of HPV-13 in oral lesions.