Ivo Bianchin

Resistência anti-helmíntica de nematóides gastrintestinais em ovinos, Mato Grosso do Sul

Among the methods of control of gastrointestinal worms in sheep, the use of chemicals is the most common. However, the continued, and indiscriminate, use of these products has selected populations of resistant helminths to anthelmintics, a phenomenon reported in the whole world. This study aimed to identify the species of gastrointestinal parasites and diagnose the status of anthelmintic resistance in sheep in the State of Mato Grosso do Sul Brazil. Feacal egg count reduction tests (FECRT) were performed in flocks of sixteen farms, and the seven formulations used contained the following pharmacological bases: Albendazole, Ivermectin, Levamizol, Trichlorfon, Moxidectin, Closantel and one containing the first three in association. The species identified at necropsy, in adult sheep, were: Haemonchus contortus, Trichostrongylus colubriformis, Cooperia curticei, C. punctata, C. pectinata and Oesophagostomum columbianum, in order of prevalence. The formulations containing Albendazole and Ivermectin did not show efficacy in reducing the EPG in the flocks tested, with average reductions of 0.7 and -19.6%, respectively. Closantel presented an average efficacy of 6.7%; Levamisolee, Moxidectin and Trichlorfon, 28.7, 26.8 and 65% respectively, the combination of three bases (Albendazole, Ivermectin and Levamizol), an average efficacy of 55.8 %. The average percentages of infective larvae recovered in the faecal cultures, pre and post treatment were similar, indicating that resistance to the bases tested is present in all species cited, to a greater or lesser degree. The two genera predominantly resistant are Haemonchus sp., with 86.9%, followed by Trichostrongylus sp., with an average of 47.5%, Strongyloides sp. 33.6%, Oesophagostomum sp. 21.4% and Cooperia sp. 19.7%.

Mosca-dos-chifres, Haematobia irritans: comportamento e danos em vacas e bezerros Nelore antes da desmama

The hornfly and losses caused by it in Nellore cows and pre-weaning calves where studied, using two groups of 60 animals each, one group with a natural infestation and the other without hornfly, during four consecutive years. It was seen that the number of flies on the cows did not exceed an average of 80 flies/animal in all years of observation. The majority of the cows (83%) had few flies, whereas 17% had larger numbers. The calves were infested with few flies. Rainfall in excess of 100 mm during short periods diminished fly infestation. Weight gain in the cows without flies (treated with insecticides) as well as their calves was greater than in the control cows, but not statistically significant (P>0.05). Mean percentage pregnancy during the four years was 15% more in treated cows than the non-treated animals, and this difference was probably due to the infestation of the bulls.

Efficacy of doramectin against field nematode infections of cattle in Latin America.

Two studies were conducted, one in Argentina and one in Brazil, to evaluate the therapeutic efficacy of doramectin administered subcutaneously at a dose rate of 200 micrograms kg-1 (1 ml per 50 kg) to cattle harboring mixed field infections of gastrointestinal nematodes. In each trial, 20 heifers were randomly allocated to a control or treated group of ten animals each, on the basis of fecal egg counts. Animals in the treated and control groups received an injection of doramectin or saline in the lateral midline of the neck. From 14 to 18 days post-treatment an equal number of animals from each group were slaughtered every day and the worm burdens of doramectin-treated animals were compared with those of non-treated controls. The efficacy of doramectin was at least 99.9% against adult stages of Ostertagia ostertagi, Haemonchus placei, Haemonchus contortus, Haemonchus similis, Trichostrongylus axei, Cooperia pectinata, Cooperia spatulata, Cooperia surnabada (Cooperia mcmasteri), Oesophagostomum radiatum and Dictyocaulus viviparus. Efficacy against Nematodirus helvetianus was 97.9% while efficacy against Trichuris discolor was 92.3%. Activity against inhibited larval forms of Ostertagia ostertagi, H. placei, Oesophagostomum radiatum, and Trichostrongylus axei, was at least 99.9%.

Sazonalidade de Haematobia irritans no Brasil Central

A mosca-dos-chifres (Haematobia irritans) e um dos principais ectoparasitos de bovinos no Brasil. Estudos epidemiologicos sao exigidos devido as dificuldades crescentes do seu controle quimico. Por isso, para um melhor conhecimento da dinâmica populacional sazonal desta mosca foram realizadas contagens a cada 14 dias sobre bovinos para corte, entre 7 e 30 meses de idade, durante o periodo de inverno de 1996 ate o verao de 2002. Foi verificada elevacao nos niveis populacionais de H. irritans (P<0,05), entre as estacoes de cada periodo de infestacao (primavera ate o outono), especialmente na primavera e verao e, as vezes, no outono, seguida de uma queda significativa (P<0,05) no inverno, quando a umidade relativa alcancou niveis inferiores a 60%. Houve uma relacao direta e imediata na contagem de moscas com a variacao da umidade relativa (P<0,05) enquanto que os efeitos mais evidentes da temperatura e a precipitacao pluvio-metrica estiveram associados com contagens feitas 42 a 56 dias depois da leitura desses dados climaticos (P<0,05). As cargas parasitarias mais elevadas (numero medio de moscas por animal) por grupo genetico estudado foram: Aberdeen Angus x Nelore = 173,5 (134-254); ½ Pardo Suico x Nelore = 183,5 (32-540); ¾ Aberdeen Angus x Nelore = 133,0 (18-374) e, Valdostana x Nelore = 219,0 (58-620). A ocorrencia de chuvas torrenciais durante a metade inicial do periodo de infestacao (primavera ate meados do verao), comum na regiao deste estudo, foi considerada a principal responsavel pelos niveis populacionais variando de moderados a baixos verificados nesta epoca em comparacao aqueles verificados no restante do periodo.

Controle alternativo de nematódeos gastrintestinais dos ruminantes: atualidade e perspectivas

Infections by gastrointestinal nematodes cause significant losses in the production of ruminants. Intensification of production process tends to enhance the effects of parasitosis. Easy access for producers to anthelmintics, in addition to poor professional assistance, has led to indiscriminate use of these chemicals and, consequently, to parasite resistance, reducing the effectiveness of treatment. Compounding the issue there is a trend towards valorization of organic products, originating from production systems where the use of chemotherapics is restricted or prohibited. This context emphasizes the importance of alternative approaches based on: management of livestock and pastures, biological control of parasites, stimulation of immunological response of host and phytotherapy. There are some promising methods by future utilization and others viable by immediate application, which, combined with conventional anthelmintics, can guarantee success and sustainability in controlling the nematodiasis.

Efeito de carrapaticidas/inseticidas "pour-on" sobre adultos do besouro coprófago africano Onthophagus gazella Fabr. (Coleoptera:Scarabaeidae)

In 1989, the dung-beetle Onthophagus gazella Fabr. was imported from the United States to be part of the integrated control program of helminths and horn-fly, Haematobia irritans L. Because there is no information about the effects of pyrethroids on the African beetle survival, the effect of four commercial pour-on insecticides for horn-fly control, on dung-beetle survival was investigated. Fifteen 24 months old, Nelore steers, were randomly assigned to five groups, with three animals/group. Animals in group 1 (control) received no treatment. Groups 2, 3, 4 and 5 were treated on day 0 with Alphamethrin, Cyalothrin, Deltamethrin and Flumethrin, respectively. Individual samples of fecal material (approximately 100 g) were collected from the rectum, on days 1, 4, 8, 11, 15 and 18 after treatment and placed in 500 ml glass vials. Ten live beetles were added in each vial and the survival rate was determined. Beetle mortality was high (P 0,01) in mortality rate among treated groups.

Efeito da mosca-dos-chifres, Haematobia irritans (L.) (Diptera: Muscidae),no ganho de peso de bovinos Nelore

This investigation was carried out due the lack of information about the possible effects of the blood-feeding horn fly, Haematobia irritans (L.), on Nellore cattle. Data were recorded during four rainy seasons (October to April) from 1991 to 1995. In each year, 80 animals were utilized according to the following treatments: twenty-eight 1-year old bulls divided into four groups; twenty 2-year old cattle divided into four groups and thirty-two 3-year old cattle divided into eight groups. Treatments against H. irritans were conduced at about 28-day intervals on half part of each age groups and the remainders groups maintained without treatment as control. The flies were counted at 14-day intervals and the animals weight registered at each 28-day intervals. The values for the mean flies number on the animals of the control groups in the first, second, third and fourth years of this study were: five, five, four and five, in the 1-year old group; 15, 11, 13 and 27, in the 2-year old group, and 55, 31, 40 and 51, in that 3-year old group, respectively. It was observed that higher number of flies (P<0.05) attack adult animals than younger ones. Despite of the low number of flies per cattle, there was a significant weight gain (P<0.05) in favour of treated groups than the controls, which gained 8.6, 16.0 and 10.4% over the period, for groups one, two and three years old, respectively. The weight gain per year was 9, 5, 7 and 8 kg for the 1-year old groups; 26, 10, 23 kg and 12 in 2-year old groups, and 16, 8, 9 and 11 kg in 3-year old groups.

Efeitos da everminação de matrizes e de bezerros lactentes em sistema de produção de bovinos de corte na região de Cerrado

The effect of deworming with ivermectin of cows before calving and of suckling calves on fecal egg counts (EPG) and productive performance of two beef cattle herds in Central Brazil was studied. Four groups of pregnant cows received the following treatments: T1- cows and calves not treated, T2- only calves treated, T3- only cows treated, and T4- cows and calves treated. The calves of T2 and T4 were distributed in the following treatments: A- calves treated at 3 to 5 months of age with long action ivermectin, B- treated with ivermectin, and C- control. For the cows, the deworming did not diminish the EPG during lactation and also did not have significant effect on the conception rate, live weight gain and the body weight of their calves at 3 to 5 months of age. The calves of treatment A gained, from the time of treatment to weaning (84 to 108 days), an average of 4.2kg (P=0.0003) and 7.1kg (P<0.0001) more than those of treatment B and C, respectively. The average difference in live weight gain of 2.9kg between the animals of treatment B and C was not significant. The EPG before treatment was not significantly different from the treatments (P=0.8665); but at weaning, the average EPG of the calves from treatment A was lower than for treatment B (P=0.0004) and C (P<0.0001). There was no significant difference in the mean EPG for the calves from treatment B and C.

Alho desidratado (Allium sativum L.) no controle de nematódeos gastrintestinais em bovinos naturalmente infectados

During 74 days, three groups of six Nelore weaned heifers, with natural mixing infections of gastro-intestinal nematodes, were housed separately and treated with 20, 10 and 0g animal-1 day-1 of dehydrated garlic added the ration. During all the experimental period no statistical difference (P>0.05) between treated and control groups was observed in egg per gram of faeces (EPG) and in relative percentage of infective larvae in the larval cultures.

Weight gain and control of endo- and ectoparasites of beef heifers treated with allopathic, herbal and homeopathic drugs.

UNLABELLED The aim of this study was to evaluate the efficacy of herbal, homeopathic and allopathic treatments for parasites in beef heifers during two experimental cycles of 318 and 313 days. TREATMENTS NC - negative control (untreated); HH - treated with homeopathic preparation Homeo bovis Parasitário®; PC - (positive control) - treated with 10% moxidectina® and an acaricide formulation of cypermethrin, chlorpyrifos and piperonyl butoxide®; HF - treated with homeopathic preparation Fator C&MC®; and FN - treated with neem cake (torta de neem®) and with neem oil (óleo de neem®). Parasite egg count (EPG), horn fly (Haematobia irritans) and tick (Rhipicephalus (Boophilus) microplus) assessment and animal weighting were performed at 28-day intervals. Blood samples were collected at the first cycle to assess the immune response. Horn fly infestation was not affected by any treatment (P>0.05). The mean number of ticks, which was low in both cycles, was lower (P<0.05) in the first cycle in animals that received PC treatment. In both experimental cycles, the mean EPG of the PC-treated animals was lower (P<0.05) than the animals receiving other treatments. TREATMENTS had no effect on the immune response (P>0.05). The animals treated with allopathic drugs were 22 to 30 kg heavier (P<0.05) than untreated animals or animals treated with alternative drugs.