J. A. Gálvez

Plastic Accumulation in the Mediterranean Sea

Concentrations of floating plastic were measured throughout the Mediterranean Sea to assess whether this basin can be regarded as a great accumulation region of plastic debris. We found that the average density of plastic (1 item per 4 m2), as well as its frequency of occurrence (100% of the sites sampled), are comparable to the accumulation zones described for the five subtropical ocean gyres. Plastic debris in the Mediterranean surface waters was dominated by millimeter-sized fragments, but showed a higher proportion of large plastic objects than that present in oceanic gyres, reflecting the closer connection with pollution sources. The accumulation of floating plastic in the Mediterranean Sea (between 1,000 and 3,000 tons) is likely related to the high human pressure together with the hydrodynamics of this semi-enclosed basin, with outflow mainly occurring through a deep water layer. Given the biological richness and concentration of economic activities in the Mediterranean Sea, the affects of plastic pollution on marine and human life are expected to be particularly frequent in this plastic accumulation region.

Morphological and physiological differences between two morphotypes of Zostera noltii Hornem. from the south-western Iberian Peninsula

Abstract The morphological and physiological differences between two morphotypes of Z. noltii Hornem. were studied in the intertidal meadows on the south-western Iberian Peninsula (Palmones river estuary and Ria Formosa). A small-leaved morphotype (SM) grows mainly at high intertidal sites, meadow edges or in recently deposited sandbanks, whereas a large-leaved morphotype (LM) generally thrives in well-structured beds or in deeper places. This study deals with the morphological, biochemical and physiological differences between these morphotypes as well as the ecological implications of the occurrence of different morphotypes in the same meadow. Shoot length, leaf width, rhizome internode length, roots per node, root length, leaf nutrient and pigment contents, and photosynthetic rates of both morphotypes were compared. The below-ground architecture (root and rhizome complex) of both morphotypes was more developed in sites characterized by higher hydrodynamics and/or a lower nitrogen content in sediments. Both morphotypes showed similar values for photosynthetic efficiency, dark respiration rate and compensation irradiance. On the other hand, the net photosynthetic capacity was much greater (5-fold) for the SM. This difference could explain the greater growth rate and faster leaf turnover rate of the SM compared with the LM. The occurrence of the SM in newly settled areas (and in the meadow edges) could be explained on the basis of its higher growth rate, which would allow a faster spreading of the meadow and/or better recovery after burial resulting from stormy weathers.

Daily, biweekly, and seasonal temporal scales of pCO2 variability in two stratified Mediterranean reservoirs

Temporal scales of variability for the partial pressure of CO2 (pCO2) in the surface waters of two stratified Mediterranean reservoirs were examined through the temporal decomposition of 5 month time series with hourly sampling frequency. pCO2 time series included similar patterns of variability at daily, biweekly, and seasonal scales regardless of the difference in amplitude of the pCO2 variation in the two reservoirs studied. Daily variability was strongly related to the day-night cycles of metabolic activity, accounting for about one third of the total amplitude in pCO2 variation. At a biweekly scale, wind forcing led to higher rates of air-water CO2 exchange and subsequently temporary partial mixing events associated to relevant increase of CO2 concentration in surface waters. Seasonal variability accounted for one third of the amplitude of the pCO2 variability and was coupled to the seasonal dynamics of water temperature and thermal stratification of the water column. Our results provide evidence that CO2 emission from stratified water bodies shows significant variability at daily, biweekly, and seasonal scales; all of which should be taken into consideration in the analyses of the carbon fluxes. The wind-induced mixing events, operating at temporal scales between daily and seasonal cycles, may become a major factor controlling the pCO2 dynamics. Hence, some of the most common models for computing CO2 fluxes from pCO2 were not able to reproduce the biweekly response patterns of CO2 emissions to wind forcing.

Growth and Biochemical Composition of the Diatom Phaeodactylum tricornutum at Different pH and Inorganic Carbon Levels under Saturating and Subsaturating Light Regimes

Abstract The effects of pH and total dissolved inorganic carbon (DIC) availability on the growth and biochemical composition of Phaeodactylum tricornutum Bohlin acclimated to restricted and saturated light conditions have been investigated. Diluted, unbuffered and nutrient enriched batch cultures were grown at several pHs (different [CO2( aq )] for the same DIC) and various DIC conditions (different [CO2( aq )] for an equal pH) under saturating (150 μmol m−2 s−1) and subsaturating (30 μmol m−2 s−1) light levels. The growth rate was unaffected by pH treatments at saturating light. In contrast, under subsaturating light conditions, the growth rate was significantly reduced at pHs over 8.5 and also at low DIC levels to a similar extent. Biochemical composition revealed that the growth rate was limited by different resources. At natural DIC (DIC = 2.1 mM) and low light conditions, there was an increase of the C:N ratio under high pH (low [CO2( aq )]) associated with a reduction of nitrogen, chlorophyll a and total protein content, resembling the biochemical composition of a diatom cultured under nitrogen limitation. On the contrary, under low light and low DIC conditions, in which both [CO2( aq )] and [HCO3 −] are reduced, cellular carbon content decreased for a relatively constant nitrogen content, decreasing the C:N ratio. The results are discussed in terms of competition between carbon and nitrogen metabolism for energy, and showed that the imposition of different [CO2( aq )] by changing pH or DIC levels of seawater affected in different ways the final biochemical composition of Phaeodactylum tricornutum.

Sediment resuspension in a monomictic eutrophic reservoir

During the mixing period sediment traps were placed at 9 different levels of the water column in La Concepción reservoir (Málaga-Spain). During the exposure time a benthic nepheloid layer with high suspended matter was detected. Seston vertical flux profile shows an exponential increase with depth, suggesting that a large fraction of the collected particulate matter comes from bottom resuspension, due to an intermittent complete mixing process. It was estimated that resuspension 1 m above the bottom increased the fluxes of seston, particulate organic carbon, particulate nitrogen and particulate phosphorus by factors of 4.1, 1.5, 2.1 and 4.8 respectively.The, fitting to an exponential function allowed us to obtain a resuspension coefficient and the scale vertical length for this event, 36 m, which is higher than those estimated for other aquatic environments. From the above results the calculated vertical eddy diffusivity, suggesting a high turbulence in the water reservoir during the turnover period. Resuspension is considered important in order to estimate net downward fluxes and to understand different processes such as redistribution of sediment and fertilization of the water column.

Microalgae cultivation in urban wastewater: Coelastrum cf. pseudomicroporum as a novel carotenoid source and a potential microalgae harvesting tool

The aim of this work was to study the optimal growth and high value-added production of the microalgae Coelastrum cf. pseudomicroporum Korshikov cultivated in urban wastewater. It was observed that C. cf. pseudomicroporum grew ideally in this medium, acting as an efficient nutrient starver. Additionally, the obtained biomass increased carotenoid cell content after saltwater stress. The effects of light intensity and salt stress on its growth rate were analysed. The results showed that this alga can grow very fast using wastewater as culture medium, reaching maximum growth rates of 1.61±0.05day-1, and tolerating strong irradiances. It was also found that under salt-stress this species could accumulate carotenoids (range 1.73-91.2pgcell-1). Moreover, a good harvesting efficiency (96.84%) was observed using Coelastrum exudates as bioflocculant of Scenedesmus sp., so Coelastrum exudates could act as a potential bioflocculant for other species.

Sedimentation and mineralization of seston in a eutrophic reservoir, with a tentative sedimentation model

Seston vertical flux was estimated by using sediment traps in a eutrophic stratified reservoir in southern Spain (Marbella, Malaga). At the same time, phytoplankton flux was estimated in the laboratory. Phytoplankton epilimnetic flux represents a high percentage of seston vertical flux. However, in hypolimnion the percentage of phytoplankton decreases, and the role of detritus becomes more important. During sedimentation, mineralization of organic matter is carrying on, and nutrient regeneration in the euphotic zone enhances primary production. Regeneration at the thermocline level is related to the presence of a deep chlorophyll maximum. A functional relationship between primary production and sedimentation produces evident regularities when different freshwater environments are compared.

Potential effects of climate change on the water level, flora and macro-fauna of a large neotropical wetland.

Possible consequences of climate change in one of the world’s largest wetlands (Ibera, Argentina) were analysed using a multi-scale approach. Climate projections coupled to hydrological models were used to analyse variability in wetland water level throughout the current century. Two potential scenarios of greenhouse gas emissions were explored, both resulting in an increase in the inter-annual fluctuations of the water level. In the scenario with higher emissions, projections also showed a long-term negative trend in water-level. To explore the possible response of biota to such water-level changes, species-area relationships of flora and aerial censuses of macro-fauna were analysed during an extraordinary dry period. Plant species richness at the basin scale was found to be highly resistant to hydrological changes, as the large dimension of the wetland acts to buffer against the water-level variations. However, local diversity decreased significantly with low water levels, leading to the loss of ecosystem resilience to additional stressors. The analysis of macro-fauna populations suggested that wetland provides refuge, in low water periods, for the animals with high dispersal ability (aquatic and migratory birds). On the contrary, the abundance of animals with low dispersal ability (mainly herbivorous species) was negatively impacted in low water periods, probably because they are required to search for alternative resources beyond the wetland borders. This period of resource scarcity was also related to increased mortality of large mammals (e.g. marsh deer) around water bodies with high anthropogenic enrichment and cyanobacteria dominance. The synergy between recurrent climatic fluctuations and additional stressors (i.e. biological invasions, eutrophication) presents an important challenge to the conservation of neotropical wetlands in the coming decades.

Planktonic community metabolism in two stratified Mediterranean reservoirs with different trophic status

Community metabolism plays a crucial role in the carbon dynamics of continental aquatic ecosystems. In the present work, two Mediterranean reservoirs (Southern Spain) with different trophic status were monitored during the stratified phase over two consecutive years (2009 and 2010) to evaluate community metabolic dynamics and potential controlling factors. Dark and light incubations were performed in order to estimate the daily rates of gross production (GP), community respiration (CR) and net community production (NCP). Temperature, irradiance, chlorophyll, phosphorus and carbon concentration were also measured at different depths within the mixed layer. On average, results showed heterotrophic behavior (GP < CR) in both reservoirs, which was particularly evident in the more eutrophic reservoir. Interannual climate variability was high due to the exceptionally rainy winter of 2010. This resulted in a significant decrease in the magnitude of the heterotrophic balance in both reservoirs. The change in NCP was especially evident in the most eutrophic reservoir where high water renewal rates lead to a lower CR. In general, NCP was found to be directly related to the ratio between the euphotic layer depth and the mixed layer depth, and inversely related to the particulate phosphorus concentration. Autotrophic behavior was only found in conditions of high irradiance (mixed layer shallower than euphotic layer), relatively low concentrations of dissolved organic carbon (<6 mg L−1) and particulate phosphorus (<10 μg L−1), and moderate chlorophyll concentration (2–9 μg L−1), albeit these conditions did not always lead to a net autotrophic balance.

Organic carbon sedimentation dominates over CO2 emission in two net heterotrophic Mediterranean reservoirs during stratification

Freshwater reservoirs are significant components of the global carbon cycle. However, relatively few studies address the balance between the atmospheric carbon flux and sedimentation in these ecosystems. We performed a simultaneous analysis of the sedimentation fluxes of particulate organic carbon (SPOC) and atmospheric CO2 fluxes (