J. Coene

Paired analysis of plasma proteins and coagulant capacity after treatment with three methods of pathogen reduction

The effect of photochemical pathogen reduction (PR) methods on plasma quality has been the subject of several reports but solid comparative data for the different technologies are lacking.

Oxygen removal during pathogen inactivation with riboflavin and UV light preserves protein function in plasma for transfusion

Photochemical pathogen inactivation technologies (PCT) for individual transfusion products act by inhibition of replication through irreversibly damaging nucleic acids. Concern on the collateral impact of PCT on the blood components integrity has caused reluctance to introduce this technology in routine practice. This work aims to uncover the mechanism of damage to plasma constituents by riboflavin pathogen reduction technology (RF‐PRT).

Evaluation of different packings for high-performance liquid chromatographic analysis of alkyl lysophospholipids

Abstract The analysis of the alkyl lysophospholipid 1-octadecyl-2-0-methyl- d , l -glycero-3-phosphorylcholine is currently under investigation because of its anticancer activity. The chromatographic behaviour of this compound and its 1-hexadecyl-2-0-methyl- d , l -glycero-3-phosphorylcholine homologue, which is used as an internal standard for pharmacokinetic studies, on various liquid chromatography packings gave rise to many problems. The retention and elution characteristics of both ether phospholipids were studied on silica, straight polyethyleneglycol-coated silica, reversed-phase materials, base-deactivated reversed-phase silica and polymeric resins.

Aggregates in platelet concentrates

P. F. van der Meer, L. J. Dumont, M. Lozano, N. Bondar, J. Wong, S. Ismay, J. Pink, W. Nussbaumer, J. Coene, H. B. Feys, V. Compernolle, D. V. Devine, D. Howe, C. K. Lin, J. Sun, J. Ringwald, E. F. Strasser, R. Eckstein, A. Seltsam, P. Perseghin, P. Proserpio, S. Wakamoto, M. Akino, S. Takamoto, K. Tadokoro, D. Teo, P. H. Shu, S. S. Chua, T. Jimenez-Marco, J. Cid, E. Castro, I. Mu~ noz, H. Gulliksson, P. Sandgren, S. Thomas, J. Petrik, K. McColl, H. Kamel, J. Dugger, J. D. Sweeney, J. B. Gorlin, L. J. Sutor, D. Heath & M. H. Sayers.

Observational study of corrected count increments after transfusion of platelets treated with riboflavin pathogen reduction technology in additive solutions

Mirasol pathogen reduction technology (PRT) treatment inactivates bacteria, viruses, and parasites in plasma products and platelets (PLTs) suspended in plasma and PLT additive solutions (PAS). Few clinical studies exist documenting transfusions with PAS. This study objective was to evaluate the count increments of PRT‐treated PAS‐C and PAS‐E buffy coat (BC) PLTs in routine use observational settings.

Gas chromatographic determination of alkyl lysophospholipids after solid-phase extraction from cell culture media

The gas chromatographic determination of 1-O-octadecyl-2-O-methyl-DL-glycero-3-phosphorylcholine (Et-18-OMe), an anti-invasive alkyl lysophospholipid, in cell culture media is described. Sample clean-up was performed by solid-phase extraction on a weak cation-exchange column of the CBA type (carboxylic acid). For quantitation, the structural analogue Et-16-OMe as the internal standard was used after derivatization with trimethylsilyl bromide. The described method was free of interferences in cell culture media. The overall precision for twenty determinations was 14.99%.

High platelet content can increase storage lesion rates following Intercept pathogen inactivation primarily in platelet concentrates prepared by apheresis

Pathogen inactivation methods for platelet concentrates are increasingly being used in blood banks worldwide. In vitro studies have demonstrated its effects on storage lesion, but little routine quality control data on blood banking outcomes have been reported.

Persistent aggregates in apheresis platelet concentrates

Aggregates often appear during apheresis. Sometimes, these persist throughout storage, causing product wastage. This study assessed product quality of apheresis concentrates containing persistent aggregates (PA) and aimed to identify the factors that contribute to their formation.

Fluorescence behaviour of an alkyl lysophospholipid in the presence of 1,6-dipheylhexatriene

Abstract The alkyl lysophospholipid (ALP), 1-O-octadecyl-2-O-methyl- dl -glycero-3-phosphorylchlorine (Et-18-OMe), which shows anticancer and antimetastatic properties, causes a very large increase in the fluorescence intensity of the known fluorescent probe, 1,6-diphenyl-1,3,5-hexatriene (DPH), when DPH enters the hydrophobic region of the ALP bilayer. This phenomenon is used to determine 2–166 μg ml−1 Et-18-OMe by adding a dilute solution of DPH in tetrahydrofuran to an aqueous analyte solution. Parameters that affect the fluorescence intensity are studied.

Comparison of three commercially available buffy coat pooling sets for the preparation of platelet concentrates

A disposable set for platelet concentrate (PC) preparation by the buffy coat method allows pooling of buffy coats, centrifugation and cell separation with in‐line leucocyte filtration. This study compares three commercially available pooling sets in combination with INTERCEPT pathogen inactivation (PI).