J. Q. Yu

Microfluidic droplet grating for reconfigurable optical diffraction.

This Letter presents a reconfigurable optical diffraction grating using multiphase droplets on a microfluidic chip. The uniform and evenly spaced circular droplets are generated by continuously dispersing two immiscible liquids into a T junction to produce plugs, which are then transformed into a circular shape at a sudden expansion of the microchannel. In experiments, the droplet grating shows a detection limit of ~6.3x10(-5) when used as an opto fl uidic refractometer and produces different colors as a color filter. Such a grating has the advantages of high stability and wide tunability in droplet size, grating period, and refractive index, making it promising for biochemical and biomaterial applications.

Fast on-demand droplet fusion using transient cavitation bubbles.

A method for on-demand droplet fusion in a microfluidic channel is presented using the flow created from a single explosively expanding cavitation bubble. We test the technique for water-in-oil droplets, which are produced using a T-junction design in a microfluidic chip. The cavitation bubble is created with a pulsed laser beam focused into one droplet. High-speed photography of the dynamics reveals that the droplet fusion can be induced within a few tens of microseconds and is caused by the rapid thinning of the continuous phase film separating the droplets. The cavitation bubble collapses and re-condenses into the droplet. Droplet fusion is demonstrated for static and moving droplets, and for droplets of equal and unequal sizes. Furthermore, we reveal the diffusion dominated mixing flow and the transport of a single encapsulated cell into a fused droplet. This laser-based droplet fusion technique may find applications in micro-droplet based chemical synthesis and bioassays.

Pulsatile shear stress and high glucose concentrations induced reactive oxigen species production in endothelial cells

A hemodynamic Lab-on-a-chip system was developed in this study. This system has two unique features: (1) it consists of a microfluidic network with an array of endothelial cell seeding sites for testing them under multiple conditions, and (2) the flow rate and the frequency of the culture medium in the microchannel are controlled by a pulsation free pump to mimic the flow profile of the blood in the blood vessel under different physiological conditions. The results demonstrate the advantage of utilizing this system over the conventional non-pulsatile system in the future shear stress related studies.