J. Raula

Oral hypoglycaemic effect of GLP-1 and DPP4 inhibitor based nanocomposites in a diabetic animal model

Glucagon-like peptide-1 (GLP-1), an incretin hormone, is used for type 2 diabetes mellitus (T2DM) treatment because of its ability to stimulate insulin secretion and release in a glucose-dependent manner. Despite of its potent insulinotropic effect, oral GLP-1 delivery is greatly limited by its instability in the gastrointestinal tract, poor absorption efficiency and rapid degradation by dipeptidylpeptidase-4 (DPP4) enzyme leading to a short half-life (~2min). Thus, a multistage dual-drug delivery nanosystem was developed to deliver GLP-1 and DPP4 inhibitor simultaneously. The system comprised of chitosan-modified porous silicon (CSUn) nanoparticles, which were coated by an enteric polymer, hydroxypropylmethylcellulose acetate succinate MF, using aerosol flow reactor technology. A non-obese T2DM rat model induced by co-administration of nicotinamide and streptozotocin was used to evaluate the in vivo efficacy of the nanosystem. The oral administration of H-CSUn nanoparticles resulted in 32% reduction in blood glucose levels and ~6.0-fold enhancement in pancreatic insulin content, as compared to the GLP-1+DPP4 inhibitor solution. Overall, these results present a promising system for oral co-delivery of GLP-1 and DPP4 inhibitor that could be further evaluated in a chronic diabetic study.

Temperature controlled release from polystyrene-block-poly(N-isopropylacrylamide-block-polystyrene block copolymer hydrogel

We report temperature controlled release from thermo-responsive polystyrene-block-poly(N-isopropyl acrylamide)-block-polystyrene triblock copolymer hydrogel. The hydrogel swelling and release of water soluble methyl orange are studied as a function of temperature for bulk and for two nano-confinements: cylindrical electrospun nanofibres and spherical aerosol nanoparticles. The time for 80% release from bulk hydrogel is found to be dependent on temperature. At room temperature the 80% release takes in 3 h whereas at 40 °C the same release takes 60 h. The swelling was found to be dependent on temperature and polymer composition.