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Featured researches published by J. Street.


Experimental and Clinical Immunogenetics | 1998

HLA class I (A, B) and II (DR, DQ) Gene and Haplotype Frequencies in Blood Donors from Wales

C. Darke; M.G. Guttridge; J. Thompson; S. McNamara; J. Street; M. Thomas

Accurate estimates of HLA-A, B, DR and DQ phenotype, gene and haplotype frequencies (HF) in the normal population are of importance in, for example, disease susceptibility studies, platelet transfusion support and transplantation. HLA population genetics studies have been performed on numerous groups, however, no major studies have been carried out on the population of Wales. As part of the validation process for our routine HLA-A and B typing by PCR using sequence-specific primers (PCR-SSP) we examined 1,798 normal, unrelated Caucasoid blood donors living in Wales and recruited onto the Welsh Bone Marrow Donor Registry (WBMDR). Typing was performed by serology (HLA-A, B) and PCR-SSP at low resolution (HLA-A, B, DR, DQ) resulting in a particularly rigorous level of HLA specificity assignment. Four discrepancies were found between the HLA-A and B serological and PCR-SSP specificity assignments: (1) two instances of HLA-A2 by serology were undetected by PCR-SSP and were a new HLA-A2 allele – A*0224; (2) one example of HLA-B*15 by PCR-SSP failed to react by serology, and remained undetectable by serology in subsequent samples, and (3) one example of HLA-B45 by serology was identified as HLA-B*5002 by PCR-SSP. Hardy-Weinberg and homozygosity analysis showed that the goodness-of-fit was excellent (p > 0.05), for both phenotype distribution and the number of homozygotes identified, for all four loci. The phenotype and gene frequencies for the 18 HLA-A, 34 -B, 15 -DR and 8 -DQ specificities identified and two- and three-locus HF, linkage disequilibrium and related values for HLA-A/B, B/DR, DR/DQ and HLA-A/B/DR and B/DR/DQ were essentially typical of a northern European population. HLA-A2, B44, DR4 and DQ2 were the highest frequency phenotypes and HLA-A2403, A34, A74, B42, B75, B2708, B48, B67 and B703 occurred once only. There were no examples of: A36, A43, A69, A80, B46, B54, B59, B73, B76, B77, B7801, B8101 or DR18 specificities. DR17, DQ2 and A1, B8, DR17 were the highest frequency two- and three-locus haplotypes identified. Diverse HLA-A, B, DR phenotypes were identified in 87.0% (1,564) of subjects. When HLA-DQ was also considered, different four locus phenotypes were identified in 89.1% (1,602) of subjects. This frequency information will be beneficial as a high-quality reference control for disease susceptibility studies and in calculating the chances of identifying a bone marrow donor in a patient’s extended family. This process was successful for the validation of our HLA-A and -B PCR-SSP typing procedure and the findings suggest an accurate level of specificity assignment of WBMDR panel donors who had previously been typed by serology alone.


European Journal of Immunogenetics | 1994

SEROLOGICAL IDENTIFICATION OF A NEW HLA-B7 VARIANT ANTIGEN –HLA-B7QUI

H. Fussell; M. Thomas; J. Street; C. Darke

A new HLA‐B antigen, HLA‐B7Qui that appears to be a variant of HLA‐B7 has been identified. This antigen, which is HLA‐Bw6 associated, reacts with approximately two‐thirds of cytotoxic antisera stimulated by HLA‐B7 or B27 that lack a B27 or B7 component, respectively. All anti‐B7+27 antisera (stimulated by either B7 or B27) react with B7Qui as do most B22‐stimulated sera possessing a B7 component. However, sera stimulated by B60, with or without a B7 component, fail to react with B7Qui.


Tissue Antigens | 2009

A new HLA-C allele: Cw*0819.

J. Johnson; J. Street; L. Hammond; J. Pepperall; C. Darke

Human leucocyte antigen-Cw*0819 differs from Cw*0802 by two nucleotides resulting in an amino acid change of 99tyrosine to 99phenylalanine and a synonymous substitution.


Tissue Antigens | 2009

A new HLA-B*08 variant--B*0838.

J. Johnson; J. Street; L. Hammond; J. Pepperall; C. Darke

HLA-B*0838 differs from B*080101 by three nucleotides resulting in an amino acid change of 63asparagine to 63glutamic acid and a synonymous substitution.


Tissue Antigens | 2009

Two new HLA-C alleles: Cw*0222 and Cw*0434

J. Johnson; J. Street; Lisa A. Hammond; C. Iley; C. Darke

HLA-Cw*0222 differs from Cw*020202 by three nucleotides at codons 114, 116 and 127, while HLA-Cw*0434 differs from Cw*0408 by two nucleotides at codons 152 and 156.


Tissue Antigens | 2015

Three new HLA‐DQB1 alleles – DQB1*03:113, DQB1*06:02:15 and DQB1*06:129

J. Street; C. Harvey; E. Cook; J. Johnson; C. Darke

Three novel HLA-DQB1 alleles were found after sequence-based typing of 3558 random UK European routine blood donors.


Tissue Antigens | 2015

A new HLA-A*26 family allele--HLA-A*26:103.

J. Street; T. Climer; J. Johnson; J. Pepperall; C. Darke

HLA-A*26:103 differs from A*26:01:01 by one base (559C>G) in exon 3 resulting in an amino acid substitution of R163G.


Tissue Antigens | 2011

A new HLA‐DQB1 sequence, DQB1*02:01:04, discovered during an external quality assessment exercise

D. Smillie; J. Street; Mats Bengtsson; S. Corbin; C. Darke

HLA-DQB1*02:01:04 differs from DQB1*02:01:01 by one nucleotide (G>A) at position 303 in exon 2 resulting in a silent substitution (codon 69 - GAG >GAA), conserved glutamate.


Tissue Antigens | 2014

A new HLA‐A*01 allele – A*01:139

J. Street; J. Johnson; J. Pepperall; C. Darke

HLA-A*01:139 differs from A*01:01:01:01 by one nucleotide (383G>C) resulting in an amino acid change of glycine104alanine.


Tissue Antigens | 2013

HLA-A*26:92—a further allele possessing a segment of A*02:01:01:01

J. Street; J. Johnson; J. Pepperall; C. Darke

HLA-A*26:92 differs from A*26:01:01 by seven bases in exon 2 resulting in six amino acid substitutions.

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Lisa A. Hammond

University of Texas Health Science Center at San Antonio

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D. Smillie

NHS Blood and Transplant

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