Jaber G. Qasem
University of Kentucky
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Featured researches published by Jaber G. Qasem.
Biochemical Pharmacology | 2002
Georg T. Wondrak; Daniel Cervantes-Laurean; Michael J. Roberts; Jaber G. Qasem; Moonsun Kim; Elaine L. Jacobson; Myron K. Jacobson
Abstract Tissue deterioration and aging have long been associated with the accumulation of chemically induced protein and DNA damage. Reactive oxygen species (ROS) and reactive carbonyl species (RCS), especially α-dicarbonyl compounds, are key mediators of damage caused by oxidative stress, glycation, and UV-irradiation. The toxic effects of ROS are counteracted in vivo by antioxidants and antioxidant enzymes, and the deleterious effects of one RCS, methylglyoxal, are counteracted by a ubiquitous glyoxalase system. Carbonyl stress as a result of toxic effects of various mono-dicarbonyls (e.g. 4-hydroxynonenal) and α-dicarbonyls (e.g. glyoxal and deoxyosones) cannot be directly antagonized by antioxidants, and only a small number of biological carbonyl scavengers like glutathione (GSH) have been identified to date. We have developed a new screening method for the identification of carbonyl scavengers using a rapid glycation system that proceeds independent of oxygen and therefore, excludes identification of inhibitory compounds acting as antioxidants. Using this screening assay adapted to 96-well microtiter plates, we have identified the cysteine derivative 3,3-dimethyl- d -cysteine as a potent inhibitor of non-oxidative advanced glycation. Comparative kinetic analyses demonstrated the superior α-oxoaldehyde-scavenging activity of d -penicillamine over that of aminoguanidine. d -Penicillamine traps α-oxoaldehydes by forming a 2-acylthiazolidine derivative as shown by structure elucidation of reaction products between d -penicillamine and methylglyoxal or phenylglyoxal. We demonstrated that upon co-incubation, d -penicillamine protects human skin keratinocytes and fibroblasts (CF3 cells) against glyoxal- and methylglyoxal-induced carbonyl toxicity. Our research qualifies α-amino-β-mercapto-β,β-dimethyl-ethane as a promising pharmacophore for the development of related α-dicarbonyl scavengers as therapeutic agents to protect cells against carbonyl stress.
Aaps Pharmscitech | 2003
Jaber G. Qasem; Paul M. Bummer; George A. Digenis
This study was designed to examine the kinetics of decomposition of paclitaxel 2′-N-methylpyridinium mesylate (PNMM), a derivative of paclitaxel. Further, the potential for PNMM to act as a prodrug of paclitaxel was assessed in vitro. Stability studies of PNMM were conducted over a pH range of 4.0 to 8.0 at 25°C. The critical micelle concentration (CMC) of PNMM was determined by pulsating bubble surfactometry. Studies of the conversion of PNMM to paclitaxel were conducted in vitro in human plasma. Decomposition of PNMM followed apparent zero-order kinetics. The pH-rate profile exhibited no evidence of acid catalysis down to pH 4.0, while the rate was accelerated under base conditions. Surface tension studies suggested that PNMM formed micelles with a CMC of approximately 34 μg/mL. Conversion studies in phosphate buffer showed that no more than 5% of PNMM converted to paclitaxel, while in human plasma the conversion was about 25%. The degradation of PNMM was via apparent zero-order kinetics and was dependent upon pH. The observed apparent zero-order kinetics of decomposition of PNMM was consistent with the formation of micelles in phosphate buffer. In buffered aqueous media alone or in human plasma, PNMM did not convert quantitatively to paclitaxel. Thus, the limiting factor in the application of PNMM as a prodrug would appear to be the poor potential to convert to paclitaxel.
Archive | 2001
Elaine L. Jacobson; Myron K. Jacobson; Hyuntae Kim; Moonsun Kim; Jaber G. Qasem
Archive | 2001
Elaine L. Jacobson; Myron K. Jacobson; Jaber G. Qasem; Hyuntae Kim; Moonsun Kim
Journal of Pharmaceutical and Biomedical Analysis | 2007
Bassam M. Tashtoush; Jaber G. Qasem; Joshua D. Williams; Tad P. DeWald; Elaine L. Jacobson; Myron K. Jacobson
Archive | 2001
Elaine L. Jacobson; Myron K. Jacobson; Jaber G. Qasem; Hyuntae Kim; Moonsun Kim
Archive | 2008
Myron K. Jacobson; Hyuntae Kim; Moonsun Kim; Elaine L. Jacobson; Jaber G. Qasem
Archive | 2001
Elaine L. Jacobson; Myron K. Jacobson; Hyuntae Kim; Moonsun Kim; Jaber G. Qasem
Archive | 2001
Elaine L. Jacobson; Myron K. Jacobson; Jaber G. Qasem; Hyuntae Kim; Moonsun Kim
Archive | 2001
Myron K. Jacobson; Hyuntae Kim; Moonsun Kim; Elaine L. Jacobson; Jaber G. Qasem