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Dive into the research topics where Jacques Rouster is active.

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Featured researches published by Jacques Rouster.


Plant Physiology | 2011

Duplicate Maize Wrinkled1 Transcription Factors Activate Target Genes Involved in Seed Oil Biosynthesis

Benjamin Pouvreau; Sébastien Baud; Vanessa Vernoud; Valérie Morin; Cyrille Py; Ghislaine Gendrot; Jean-Philippe Pichon; Jacques Rouster; Wyatt Paul; Peter M. Rogowsky

WRINKLED1 (WRI1), a key regulator of seed oil biosynthesis in Arabidopsis (Arabidopsis thaliana), was duplicated during the genome amplification of the cereal ancestor genome 90 million years ago. Both maize (Zea mays) coorthologs ZmWri1a and ZmWri1b show a strong transcriptional induction during the early filling stage of the embryo and complement the reduced fatty acid content of Arabidopsis wri1-4 seeds, suggesting conservation of molecular function. Overexpression of ZmWri1a not only increases the fatty acid content of the mature maize grain but also the content of certain amino acids, of several compounds involved in amino acid biosynthesis, and of two intermediates of the tricarboxylic acid cycle. Transcriptomic experiments identified 18 putative target genes of this transcription factor, 12 of which contain in their upstream regions an AW box, the cis-element bound by AtWRI1. In addition to functions related to late glycolysis and fatty acid biosynthesis in plastids, the target genes also have functions related to coenzyme A biosynthesis in mitochondria and the production of glycerol backbones for triacylglycerol biosynthesis in the cytoplasm. Interestingly, the higher seed oil content in ZmWri1a overexpression lines is not accompanied by a reduction in starch, thus opening possibilities for the use of the transgenic maize lines in breeding programs.


Current Biology | 2012

Maternal Control of Nutrient Allocation in Plant Seeds by Genomic Imprinting

Liliana M. Costa; Jing Yuan; Jacques Rouster; Wyatt Paul; Hugh G. Dickinson; José F. Gutierrez-Marcos

Imprinted genes are commonly expressed in mammalian placentas and in plant seed endosperms, where they exhibit preferential uniparental allelic expression. In mammals, imprinted genes directly regulate placental function and nutrient distribution from mother to fetus; however, none of the >60 imprinted genes thus far reported in plants have been demonstrated to play an equivalent role in regulating the flow of resources to the embryo. Here we show that imprinted Maternally expressed gene1 (Meg1) in maize is both necessary and sufficient for the establishment and differentiation of the endosperm nutrient transfer cells located at the mother:seed interface. Consistent with these findings, Meg1 also regulates maternal nutrient uptake, sucrose partitioning, and seed biomass yield. In addition, we generated an imprinted and nonimprinted synthetic Meg1 ((syn)Meg1) dosage series whereby increased dosage and absence of imprinting both resulted in an unequal investment of maternal resources into the endosperm. These findings highlight dosage regulation by genomic imprinting as being critical for maintaining a balanced distribution of maternal nutrients to filial tissues in plants, as in mammals. However, unlike in mammals, Meg1 is a maternally expressed imprinted gene that surprisingly acts to promote rather than restrict nutrient allocation to the offspring.


Molecular Plant-microbe Interactions | 1999

Involvement of cAMP and protein kinase A in conidial differentiation by Erysiphe graminis f. sp. hordei.

Alison Hall; Lene Bindslev; Jacques Rouster; Søren W. Rasmussen; Richard P. Oliver; Sarah J. Gurr

Erysiphe graminis f. sp. hordei, the causal agent of barley powdery mildew, is an obligate biotroph. On arrival on the host, a primary germ tube (PGT) emerges from the conidium. An appressorial germ tube (AGT) then appears, forms an appressorium, and effects host penetration. Such developmental precision may be due to multiple, plant-derived signals and to endogenous tactile and chemical signals. The transduction mechanism remains obscure. The isolation of an expressed sequence tag (EST) homologue of the catalytic subunit of cyclic AMP (cAMP)-dependent protein kinase A (PKA) enabled the corresponding gene to be characterized and the transcript to be identified in conidia and in PGT and AGT stage spores. cAMP-dependent PKA activity was detected in ungerminated conidia. These data suggest that PKA and cAMP are involved in conidial development. To substantiate this we exploited the responses of developing conidia to various surfaces, including exposure to the host leaf (fully inductive to AGT formation), cellulose membrane (semi-inductive), and glass (non-inductive). Assessment of fungal development, following application of exogenous cAMP or cAMP analogues, revealed that, at different concentrations and on different surfaces, cAMP either promoted or inhibited conidial differentiation. Various PKA inhibitors were tested for their effect on PKA activity and conidial development. A negative correlation was established between PKA inhibition in vitro and fungal development in vivo. Taken collectively, these data suggest that PKA and cAMP play a role in conidial differentiation in this obligate, plant-pathogenic fungus.


Plant Journal | 1997

Identification of a methyl jasmonate-responsive region in the promoter of a lipoxygenase 1 gene expressed in barley grain.

Jacques Rouster; Robert Leah; John Mundy; Verena Cameron-Mills


Fungal Genetics and Biology | 2001

Gene identification in the obligate fungal pathogen Blumeria graminis by expressed sequence tag analysis.

Stephen W. Thomas; Søren W. Rasmussen; Mikkel A. Glaring; Jacques Rouster; Solveig K. Christiansen; Richard P. Oliver


Plant Physiology | 1998

Substrate Specificity of Barley Cysteine Endoproteases EP-A and EP-B

Anne Davy; Ib Svendsen; Susanne Sørensen; Mikael Blom Sørensen; Jacques Rouster; Morten Meldal; David J. Simpson; Verena Cameron-Mills


Plant Physiology | 1989

Host-Pathogen Interactions: XXXII. A Fungal Glucan Preparation Protects Nicotianae against Infection by Viruses

Marguerite Kopp; Jacques Rouster; Bernard Fritig; Alan G. Darvill; Peter Albersheim


Biochimica et Biophysica Acta | 1995

Primary structure of a lipoxygenase from barley grain as deduced from its cDNA sequence.

J.R. van Mechelen; M. Smits; Anneke C. Douma; Jacques Rouster; Verena Cameron-Mills; F. Heidekamp; B.E. Valk


Plant Journal | 1998

The untranslated leader sequence of the barley lipoxygenase 1 (Lox1) gene confers embryo-specific expression

Jacques Rouster; Jan van Mechelen; Verena Cameron-Mills


Archive | 2015

Method for plant improvement

Wyatt Paul; Jacques Rouster

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Wyatt Paul

University of Leicester

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Bernard Fritig

Centre national de la recherche scientifique

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Marguerite Kopp

Centre national de la recherche scientifique

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Jing Yuan

University of Warwick

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