Jae-Gun Sunwoo

Loss of RUNX3 is significantly associated with advanced tumor grade and stage in endometrial cancers

Loss of runt-related transcription factor 3 (RUNX3) has been reported in various cancers, and one of the mechanisms mediating loss of RUNX3 expression is DNA methylation. However, the role of RUNX3 expression and its DNA methylation status as prognostic factors in endometrial cancer remain unclear. In the present study, the expression and promoter methylation of RUNX3 was examined in endometrial cancer tissues and cell lines, as well as their association with endometrial cancer prognosis. Fifty-five endometrial cancer tissues and two endometrial cancer cell lines (HEC1-α and Ishikawa) were studied. RUNX3 expression and promoter methylation were examined using reverse transcription-polymerase chain reaction (RT-PCR), methylation specific PCR (MS-PCR), and immunohistochemical staining. The demethylating agent 5-aza-2′-deoxycytidine (ADC) was used to reverse the methylation of the RUNX3 promoter. Loss of RUNX3 expression was observed in 50.9% (27/53) of endometrial cancer tissues and in the HEC1-α cell line by immunohistochemistry and RT-PCR, respectively. Methylation of the RUNX3 promoter was observed in 62.2% (33/53) of endometrial cancer tissues, 12.5% (1/8) of normal endometrial tissues, and the HEC1-α cell line by MS-PCR. Tumor grade and stage were significantly correlated with loss of RUNX3 expression. The expression of RUNX3 was restored by treatment with ADC and resulted in growth inhibition in HEC1-α cells. The present results suggested that methylation may serve a critical role in the silencing of RUNX3 and loss of RUNX3 expression may serve as a prognostic marker in endometrial cancer.

A case of treatment using skin flap of recurrent mesh erosion in transobturator tape

생활수준이 높아지면서 과거에는 중요한 질병으로 여기지 않았던 하 부요로의 대표적인 장애인 요실금으로 산부인과를 찾는 환자가 늘어나 면서 치료 지식이 필요하게 되었다. 우리나라의 연구에 의하면 요실금 의 유병률은 22.9%이고, 그 중 복압성 요실금이 64.4%, 절박성 요실금 이 20.8%이며, 다른 보고에는 성인 여성의 45%에서 요실금을 경험하 였다고 한다[1,2]. 이렇게 많은 복압성 요실금의 최근 치료로 경폐쇄공 질테이프술(transobturator tape, TOT)이 가장 각광을 받고 있다. 그러 나, 그 후 합병증의 보고가 계속 이어지고 있다. DeSouza 등[3]이 TOT 후 발생한 내전근의 근염을 보고하면서 해부학적 구조물의 술 후 감염 에 의한 합병증이 처음으로 보고되었고, 국내에서도 TOT 후 테이프의 감염에 인한 합병증에 대한 보고가 있었다[4]. 저자들도 최근 52세 복 압성 요실금 환자에서 TOT 후 반복되는 질 미란을 성형외과와의 협진 에 의해 피부판 이식과 이물질의 충분한 제거를 통해 치료한 1예를 경 험하였기에 간단한 문헌 고찰과 함께 보고하는 바이다.