Jaeman Bae

Berberine Inhibited the Growth of Thyroid Cancer Cell Lines 8505C and TPC1

Purpose Thyroid cancer is the most common malignancy in Korean females and can be treated with good prognosis. However, drugs to treat aggressive types of thyroid cancer such as poorly differentiated or anaplastic thyroid cancer have not yet been established. To that end, we analyzed the effects of berberine on human thyroid cancer cell lines to determine whether this compound is useful in the treatment of aggressive thyroid cancer. Materials and Methods The two thyroid cancer cell lines 8505C and TPC1, under adherent culture conditions, were treated with berberine and analyzed for changes in cell growth, cell cycle duration, and degree of apoptosis. Results Following berberine treatment, both cell lines showed a dose-dependent reduction in growth rate. 8505C cells showed significantly increased levels of apoptosis following berberine treatment, whereas TPC1 cells showed cell cycle arrest at the G0/G1 phase. Immunobloting of p-27 expression following berberine treatment showed that berberine induced a little up-regulation of p-27 in 8505c cells but relatively high up-regulation of p-27 in TPC1 cells. Conclusion These results suggest that berberine treatment of thyroid cancer can inhibit proliferation through apoptosis and/or cell cycle arrest. Thus, berberine may be a novel anticancer drug for the treatment of poorly differentiated or anaplastic thyroid cancer.

Berberine-induced growth inhibition of epithelial ovarian carcinoma cell lines

Aim:  The aim of this study is to analyze the impact of berberine on the two human epithelial ovarian carcinoma cell lines OVCAR‐3 and SKOV‐3 in relation to the potential usefulness of berberine in the treatment of epithelial ovarian cancer.

Transvaginal laparoscopic surgery for ovarian cysts

To evaluate the effectiveness and feasibility of transvaginal laparoscopic surgery (TLS) using endoscopic instruments for management of ovarian cysts.

The effect of Lactobacillus casei extract on cervical cancer cell lines

Aim of the study Lactobacillus casei (L. casei) has been shown to inhibit the proliferation of several types of cancer in vivo, but its effect on cervical cells has not been reported. We incubated cells of the human cervical cell lines Caski and HeLa with extracts of L. casei and investigated its effects on the growth of the cells and possible synergy with anticancer drugs. Material and methods Cell-free extracts of L. casei were prepared and purified. Cultures of Caski and HeLa cells adhering to tissue culture plates were treated with L. casei extract. The effects of L. casei extract on the growth of cancer cells and its possible synergy with anti-cancer drugs in cervical cancer cell lines were investigated. The cells were treated with L. casei extract alone, anti-cancer drugs alone [doxorubicin, paclitaxel, 5-fluorouracil (5-FU), and cisplatin], or L. casei extract plus anti-cancer drugs. Results L. casei extract had no significant effect on the growth rate of the two cell lines. Anti-cancer drugs alone induced growth inhibition, but there was no synergistic effect of L. casei extract on growth inhibition. Conclusions L. casei extract does not have a potent effect on the viability of cervical cancer cells in vitro. In addition, L. casei extract has no synergistic effect on the inhibition of growth of cancer cells in the presence of anti-cancer drugs.

Cryopreservation of Human Adipose Tissues using Human Plasma

Human adipose tissue or derived cells have been used clinically in several fields. Animal serum is used in storing and culturing human adipose tissue or derived cells. Thus far, few problems have been associated with these sera in current research fields. However, animal sera may cause some problems in clinical applications because of the toxic viruses and proteins that can be present in animal sera. Human plasma can resolve these problems associated with animal serum. In this study, we investigated the effects of human plasma as a cryopreservation solution for human adipose tissues. We observed that adherent cells derived from adipose tissues were isolated effectively by collagenase treatment. We did not observe any adherent cells when human adipose tissues were preserved without cryopreservation solution at ?20°C. Adherent adipose-derived cells exhibited different growth rates based on the cryopreservation solution and storage temperature. We also did not observe adherent cells when human adipose tissues were preserved with cryopreservation solution at ?20°C and ?70°C. Adherent adipose tissue-derived cells exhibited a similar growth rate as cryopreserved tissues when cryopreserved in 10% dimethyl sulfoxide (DMSO) + 90% fetal bovine serum (FBS) or 10% DMSO + 90% human plasma cryopreservation solution but exhibited a low growth rate when cryopreserved in 10% DMSO + 10% FBS + 80% Dulbecco’s modi?ed Eagle’s medium (DMEM) cryopreservation solution. In conclusion, these results demonstrated that human plasma is useful as a cryopreservation solution for human adipose tissues.

Comparison of the clinical performance of restriction fragment mass polymorphism (RFMP) and Roche linear array HPV test assays for HPV detection and genotyping.

BACKGROUND The need for accurate genotyping of human papillomavirus (HPV) infections is becoming increasingly important as HPV is the primary cause of cervical cancer worldwide. The matrix-assisted laser desorption ionization time-of-flight mass spectrometry-based restriction fragment mass polymorphism (RFMP) assay provides accurate, broad-spectrum, high-throughput genotyping of HPV. OBJECTIVES We evaluated the clinical performance of the RFMP assay compared to a commercially available Roche linear array HPV genotyping test (LA) for detecting and genotyping of HPV. STUDY DESIGN The RFMP assay and the LA were compared for detecting and genotyping HPV among a cohort of 244 liquid-based cytology samples. RESULTS Overall, 216 specimens (93.1%, κ = 0.86) generated concordant results for the presence or absence of high-risk HPV (HR-HPV) by the two assays. The RFMP assay and the LA assay generated concordant, compatible, and discordant genotyping results for 79.3, 9.9, and 10.8%, respectively. The diagnostic sensitivity and specificity of RFMP and LA for the cervical lesions of squamous cell carcinoma (SCC) were similar, at 92.9 and 85.0% (RFMP) and 92.9 and 83.8% (LA), respectively. In addition, the odds ratio for SCC with HR-HPV positivity estimated by the RFMP assay (73.7, 95% CI: 8.9-3173.3) was higher than the LA assay (67.0, 95% CI: 8.2-2887.0). CONCLUSIONS The RFMP and the LA assays were highly comparable with regard to detection and genotyping analysis of HPV. The sensitivity and specificity of RFMP assay for the detection of HR-HPV in various levels of cervical lesions seems to be valuable in the monitoring of HPV-associated cervical cancer.

RISK FACTORS OF LYMPHOCELE AFTER RAH WITH PELVIC LYMPH NODE DISSECTION FOR WOMEN WITH CERVICAL CANCER

목적 자궁경부암 환자에서 근치적 자궁절제술 및 골반림프절절제술 시행 후 발생하는 림프낭종의 위험인자에 대해 분석해 보았다. 연구방법 2005년 4월부터 2010년 6월까지 단일기관에서 자궁경부암으로 진단받고 근치적 자궁절제술 및 골반림프절절제술을 시행받은 병력이 있는 62명의 환자의 의무기록을 후향적으로 분석하였다. 림프낭종의 발생 여부는 초음파 혹은 골반 및 복부 전산화단층촬영을 통하여 확인하였다. 환자군의 나이, 체질량지수, 수술한 의사, 과거 수술력, 병기, 종양의 조직형, 획득한 림프절의 개수, 방사선치료 여부 등에 대하여 조사하였다. 이변량 분석 및 다변량 분석을 통하여 림프낭종에 대한 위험인자를 평가하였다. 결과 평균 추적관찰 기간 34.5개월(범위, 12-69개월) 동안 62명의 환자 중 20명(32%)에서 림프낭종이 있었고, 그 중 증상이 있는 경우가 8명 이었다. 단변량 분석 결과 방사선치료 병력과 획득한 림프절의 개수가 많을수록 림프낭종의 발생률이 유의하게 높았다. 로지스틱 회귀 분석을 통해 방사선치료 병력과 획득한 림프절의 개수가 림프낭종에 대한 독립적인 위험인자임을 보였다. 결론 방사선치료 병력과 획득한 림프절의 개수는 근치적 자궁절제술 및 골반림프절절제술을 시행받은 자궁경부암 환자에서의 림프낭종의 발생과 유의한 연관성을 보였다.

P486 Transvaginal natural orifice transluminal endoscopic surgery (NOTES) for ovarian cyst

Materials and Methods: We used an Alexis wound retractor (Applied Medical) and a surgical glove as the single 3 channel port. A 1.2-cm vertical intra-umbilical skin incision was made, and 1.5-cm rectus fasciotomy was done to enter the peritoneal cavity. The Alexis wound retractor was inserted through the incision, stretching the fascial diameter to 1.5 cm. A surgical glove was fixed to the outer ring of the wound retractor. After making a hole in 1 finger of the surgical glove, a 5-mm trocar was inserted and advanced into the abdominal cavity. The abdomen was insufflated with CO2 gas of about 2 L and a rigid 30 , 5mm laparoscope was inserted. After inspection of the pelvic cavity, we made 2 more holes in the finger of the surgical glove for a 5mm port for operating instruments and a 10 to 11mm port for passing 1/0 vicryl sutures. Results: Since Dec 2008, a total of 15 patients have undergone single port TLH (micro-invasive cervical cancer in 1, carcinoma in situ of the uterine cervix in 3, myoma in 8, adenomyosis in 1, persistent GTT in 1, placental site trophoblastic tumor in 1) using a single 3 channel port. The median total operative time and weight of uterus were 115 min (range: 57 to 150 min) and 212g (range: 49 to 400 g). The median hemoglobin level decrease on postoperative day 3 was 2.1 g/dL (range: −1.1 to 3.8 g/dL). The median postoperative stay was 3 days (range: 2 to 5 days). We have been able to perform TLH without any complications including ureteric, bladder, bowel, and vascular injuries. All patients had good postoperative recovery. Conclusions: TLH using a transumbilical single 3 channel port and conventional laparoscopic instruments is a technique that could be considered by surgeons experienced in laparoscopic operation.