James F. Walgenbach

Effects of Production Practices on Soil-Borne Entomopathogens in Western North Carolina Vegetable Systems

Abstract Populations of endemic soil entomopathogens (nematodes and fungi) were monitored in vegetable production systems incorporating varying degrees of sustainable practices in Fletcher, NC. Two tillage types (conventional plow and disk versus conservation tillage), two input approaches (chemically versus biologically based), and two cropping schedules (continuous tomato versus 3-yr rotation of corn, cucumber, cabbage, and tomato) were employed in large plots from 1995 to 1998. A Galleria mellonella (L.) trap bioassay was used to identify and monitor activity of Steinernema carpocapsae, Heterorhabditis bacteriophora, Beauveria bassiana, and Metarhizium anisopliae populations during the vegetable growing season (April–September). Seasonal detection of entomopathogens was significantly higher in conservation compared with conventional tillage systems. The strip-till operation did not affect levels of detection of S. carpocapsae. Pesticide use significantly reduced detection of entomopathogenic fungi. Type of ground cover significantly affected temperature in the upper 12 cm of soil; highest soil temperatures were observed under black plastic mulch and bare ground, whereas lowest temperatures were observed under rye mulch and clover intercrop. The high soil temperatures associated with certain ground covers may have reduced entomopathogen detection or survival. Although type of tillage appeared to be the primary factor affecting survival of endemic soil entomopathogens in our system, other factors, such as pesticide use and type of ground cover, can negate the positive effects of strip-tillage.

Variation in thrips species composition in field crops and implications for tomato spotted wilt epidemiology in North Carolina

Thrips were surveyed in tomato spotted wilt‐susceptible crops in five areas across North Carolina. Tomato, pepper, and tobacco plants in commercial fields were sampled and 30 species of thrips were collected over a 3‐year period. The most common species overall was Frankliniella tritici (Fitch). The most common thrips species that are known to vector Tomato Spotted Wilt Virus (TSWV) were F. fusca (Hinds), and F. occidentalis (Pergande). Relatively low numbers of Thrips tabaci Lindeman, another reported vector, were collected. The spatial and temporal occurrence of vectors varied with sampling method, crop species, region of North Carolina, and localized areas within each region. In a laboratory experiment, no difference was detected between the ability of F. fusca and F. occidentalis to acquire and transmit a local isolate of TSWV. Based on vector efficiency and occurrence, F. fusca is considered the most important vector of TSWV in tobacco, whereas both F. fusca and F. occidentalis are important vectors of TSWV in tomato and pepper.

Evaluation of extended-season mating disruption of the Oriental fruit moth Grapholita molesta (Busck) (Lep., Tortricidae) in apples

Abstract:  Oriental fruit moth Grapholita molesta (Busck) (Lep., Tortricidae) has recently become a key pest of apples throughout the eastern USA. Pheromone‐mediated mating disruption of Oriental fruit moth was successfully used in North Carolina apple orchards in the past few years. However, low levels of late‐season fruit damage occurred in some orchards treated in late May with hand‐applied pheromone dispensers because of inadequate dispenser longevity. To investigate alternative pheromone application schedules for extended mating disruption control, the following pheromone treatments were compared with conventional insecticides in Henderson County (NC) in 2002: late May application of hand‐applied dispensers; late June application of hand‐applied dispensers; late May application of hand‐applied dispensers supplemented with a late August application of sprayable pheromone dispensers; late May application of hand‐applied dispensers which have a longer activity period; and conventional insecticides as a control. All treatments were sprayed with an insecticide at petal fall in late April for thinning and for control of the first generation Oriental fruit moth adults. Pheromone trap catches were significantly reduced in all mating disruption blocks compared with conventional insecticide blocks. Among pheromone treatments, the highest trap captures were recorded in the delayed hand‐applied dispenser treatment in June before treatment. However, the mean percentage fruit damage did not vary with timing of application of hand‐applied dispensers and the type of pheromone dispenser used. Clearly, the combination of each mating disruption treatment with insecticide application against first generation Oriental fruit moth was as effective as the conventional insecticide treatment under moderate population pressure.

Comparison of Colored Sticky Traps for Monitoring Thrips Populations (Thysanoptera: Thripidae) in Staked Tomato Fields

The response of flower thrips, Frankliniella spp., to various colors and sticky trap designs was evaluated in staked tomato fields in western North Carolina. Yellow sticky traps caught significantl...

Attraction of the invasive halyomorpha halys (Hemiptera: Pentatomidae) to traps baited with semiochemical stimuli across the United States

ABSTRACT A recent identification of the two-component aggregation pheromone of the invasive stink bug species, Halyomorpha halys (Stål), in association with a synergist, has greatly improved the ability to accurately monitor the seasonal abundance and distribution of this destructive pest. We evaluated the attraction of H. halys to black pyramid traps baited with lures containing the pheromone alone, the synergist methyl (2E,4E,6Z)-decatrienoate (MDT) alone, and the two lures in combination. Traps were deployed around areas of agricultural production including fruit orchards, vegetables, ornamentals, or row crops in Delaware, Maryland, North Carolina, New Jersey, New York, Ohio, Oregon, Pennsylvania, Virginia, and West Virginia from mid-April to mid-October, 2012 and 2013. We confirmed that H. halys adults and nymphs are attracted to the aggregation pheromone season long, but that attraction is significantly increased with the addition of the synergist MDT. H. halys adults were detected in April with peak captures of overwintering adults in mid- to late May. The largest adult captures were late in the summer, typically in early September. Nymphal captures began in late May and continued season long. Total captures declined rapidly in autumn and ceased by mid-October. Captures were greatest at locations in the Eastern Inland region, followed by those in the Eastern Coastal Plain and Pacific Northwest. Importantly, regardless of location in the United States, all mobile life stages of H. halys consistently responded to the combination of H. halys aggregation pheromone and the synergist throughout the entire season, suggesting that these stimuli will be useful tools to monitor for H. halys in managed systems.

Effects of production system on vegetable arthropods and their natural enemies

Abstract Populations of foliar insect pests and natural enemies were monitored in vegetable production systems incorporating varying degrees of sustainable practices in Fletcher, NC, USA. Two types of tillage (conventional plow and disk, strip-tillage), two input approaches (chemically-based, biologically-based) and two cropping schedules (continuous tomato ( Lycopersicon esculentum Mill.), 3-year rotation of corn ( Zea mays L.), cucumber ( Cucumis sativus L.) and tomato) were employed from 1995 to 1998. Tomato pest pressure was relatively low in all years, resulting in a limited impact of production systems on potato aphid, Macrosiphum euphorbiae (Thomas) (Homoptera: Aphididae), and its associated parasitoids and predators. Thrips ( Frankliniella spp. (Thysanoptera)) populations were significantly higher in the biological input treatments in 3 of 4 years. Lepidopterous (primarily Helicoverpa zea Boddie (Lepidoptera: Noctuidae)) damage on tomato was significantly higher in biological treatments in all years, damage by thrips and pentatomids (Hemiptera: Pentatomidae) increasing each year in the continuous tomato crop schedule. Most insect populations were significantly influenced by type of insecticide input or ground cover. Few population measurements were affected by tillage type. Foliar insect problems in commercial vegetable production may be associated predominantly with insecticide input (i.e. more damage with biologically based insecticides) and use of intercropping (i.e. more damage in systems with living mulch); however, the long term effects of tillage and crop rotation remain to be seen.

Comparison of Mating Disruption with Pesticides for Management of Oriental Fruit Moth (Lepidoptera: Tortricidae) in North Carolina Apple Orchards

Abstract The efficacy of mating disruption by using Isomate-M 100 pheromone dispensers and two formulations of microencapsulated sprayable pheromone for management of oriental fruit moth, Grapholita molesta (Busck), was compared with conventional insecticides in large plot studies in Henderson County, North Carolina, in 2000 and 2001. In addition, experiments were conducted in small and large plots to test the response of oriental fruit moth males to different application rates of sprayable pheromone. Pheromone trap catches were significantly reduced in mating disruption blocks compared with conventional and abandoned orchards. Pheromone traps placed in the upper canopy captured significantly more moths than traps placed in the lower canopy across all treatments, and lures loaded with 100 μg of pheromone caught more moths than traps with 300 μg, but the difference between doses was statistically significant at only one location in 2001. Isomate-M 100 provided excellent trap shutdown and was significantly more effective than sprayable pheromone formulations. Fruit damage by oriental fruit moth larvae was very low (≤1%) in mating disruption blocks and was generally lower than in conventional and nonmanaged blocks. Based on male moth response to pheromone traps in small plots, there was little difference among doses of sprayable pheromone, ranging from 12.4 to 49.1 g (AI)/ha, but efficacy declined at 2.4 g (AI)/ha. With the exception of one orchard, there was no significant difference between 12.4 and 37.1 g (AI)/ha under low and high oriental fruit moth population pressure in large plot studies. Mating disruption proved to be an alternative to organophosphate insecticides for managing oriental fruit moth populations in North Carolina apple orchards.

Effects of vegetable production system on epigeal arthropod populations

Abstract Populations of epigeal arthropods were monitored in vegetable production systems under varying degrees of sustainable agricultural practices in Fletcher, NC (USA). Two tillage types (conventional plow and disk, strip-tillage (ST)), two input approaches (chemically based, biologically based) and two cropping schedules (continuous tomato Lycopersicon esculentum Mill.], 3-year rotation of sweet corn [Zea mays L.]/cabbage [Brassica oleracea L.], cucumber [Cucumis sativus L.]/cabbage and tomato) were employed from 1995–1998. A second study with tomatoes was performed in 1997–1998 to separate effects of pesticide use, intercropping and herbicide application. Pitfall traps (48-h sample period) were used at ∼25-day intervals to monitor relative activity of carabid beetles (Coleoptera: Carabidae), staphylinid (Coleoptera: Staphylinidae) beetles and lycosid spiders (Araneidae: Lycosidae). Carabids and lycosids appeared to be more active in systems with ground cover. Trap catches of carabid species were not significantly affected by insecticide input, but trap catches of lycosids were lower in plots with conventional insecticide use. No consistent effect of tillage was found over time, although Scarites spp. were more active in minimally disturbed habitats in 1998. Two distinct patterns of seasonal activity were observed for carabid beetles and lycosid spiders. Ground cover generally enhanced abundance of carabids and lycosids, while tillage type, pesticide use and crop rotation had different effects.

Evaluation of Pheromone-Based Management Strategies for Dogwood Borer (Lepidoptera: Sesiidae) in Commercial Apple Orchards

ABSTRACT The dogwood borer, Synanthedon scitula (Harris) (Lepidoptera: Sesiidae), is a serious wood-boring pest of apple in eastern North America. The recent identification of its sex pheromone and systematic documentation of the effect of a potent behavioral antagonist affords the opportunity to develop pheromone-based management strategies for this important pest. Here we evaluated the potential of pheromone-based mass trapping of males to reduce dogwood borer infestations and conducted preliminary evaluations of an antagonist-based pheromone blend for disruption of dogwood borer mate finding in commercial apple orchards in North Carolina, Virginia, and West Virginia. In the mass trapping study, treatments included a conventional trunk-drench application of chlorpyrifos, a low-density mass trapping regime of 5 traps/ha, a higher-density mass trapping regime of 20 traps/ha, and an untreated control. We removed large numbers of males from orchards at all locations, with 27,155, 8,418, and 7,281 removed from high-density trapping plots in North Carolina, Virginia, and West Virginia, respectively, over 2 yr. After 2 yr under each of these treatment regimes, infestation in high- and low-density mass trapping plots was not reduced to the level of chlorpyrifos-treated plots. An antagonist-based dispenser deployed at a rate of 250/ha effectively disrupted mate-finding by male dogwood borer. In plots with mating disruption dispensers, captures in pheromone-baited traps were virtually eliminated, and no males were captured in traps baited with virgin females.

Acaricidal Properties of Spinosad Against Tetranychus urticae and Panonychus ulmi (Acari: Tetranychidae)

Laboratory bioassays were conducted to characterize the activity of the insecticide spinosad against the twospotted spider mite, Tetranychus urticae Koch, and European red mite, Panonychus ulmi (Koch) (Acari: Tetranychidae). T. urticae females and larvae were individually placed on bean, Phaseolus vulgaris L. (Fabaceae), leaf disks treated with four rates of spinosad (25, 55, 121, and 266 ppm) and a water control. Significantly fewer T. urticae completed development on any spinosad rates (<15%) compared with the control (>85%), whereas spinosad exhibited no significant effects on P. ulmi development; 72.5 and 83.1% of P. ulmi completed development on apple (Malus pumila P. Mill, Rosaceae) leaf disks treated with 75 ppm spinosad and the control, respectively. T. urticae adult females placed on spinosad-treated disks had significantly higher mortality and lower oviposition rates compared with the water control; no significant mortality effects were observed until 3 d after placing adults on leaf disks. In choice tests where half of a bean leaf was treated with 55 ppm spinosad transversally or longitudinally, T. urticae females were repelled by spinosad and largely oviposited and fed on nonspinosad treated areas. Spinosad did not affect the behavior of P. ulmi females. When T. urticae females were released on potted bean plants (two-leaf stage) in which leaves received spinosad sprays on the adaxial or abaxial leaf surfaces, or complete spinosad coverage on one or two of the leaves, mite population increase lagged significantly behind those released on control plants. These results indicate that spinosad has significant acaricidal effects against T. urticae but not P. ulmi.