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Dive into the research topics where James G. Truslow is active.

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Featured researches published by James G. Truslow.


Biomaterials | 2010

Effect of mechanical factors on the function of engineered human blood microvessels in microfluidic collagen gels

Gavrielle M. Price; Keith H. K. Wong; James G. Truslow; Alexander D. Leung; Chitrangada Acharya; Joe Tien

This work examines how mechanical signals affect the barrier function and stability of engineered human microvessels in microfluidic type I collagen gels. Constructs that were exposed to chronic low flow displayed high permeabilities to bovine serum albumin and 10 kDa dextran, numerous focal leaks, low size selectivity, and short lifespan of less than one week. Higher flows promoted barrier function and increased longevity; at the highest flows, the barrier function rivaled that observed in vivo, and all vessels survived to day 14. By studying the physiology of microvessels of different geometries, we established that shear stress and transmural pressure were the dominant mechanical signals that regulated barrier function and vascular stability, respectively. In microvessels that were exposed to high flow, elevation of intracellular cyclic AMP further increased the selectivity of the barrier and strongly suppressed cell proliferation. Computational models that incorporated stress dependence successfully predicted vascular phenotype. Our results indicate that the mechanical microenvironment plays a major role in the functionality and stability of engineered human microvessels in microfluidic collagen gels.


Biomaterials | 2010

The role of cyclic AMP in normalizing the function of engineered human blood microvessels in microfluidic collagen gels

Keith H. K. Wong; James G. Truslow; Joe Tien

Nearly all engineered tissues must eventually be vascularized to survive. To this end, we and others have recently developed methods to synthesize extracellular matrix-based scaffolds that contain open microfluidic networks. These scaffolds serve as templates for the formation of endothelial tubes that can be perfused; whether such microvascular structures are stable and/or functional is largely unknown. Here, we show that compounds that elevate intracellular concentrations of the second messenger cyclic AMP (cAMP) strongly normalize the phenotype of engineered human microvessels in microfluidic type I collagen gels. Cyclic AMP-elevating agents promoted vascular stability and barrier function, and reduced cellular turnover. Under conditions that induced the highest levels of cAMP, the physiology of engineered microvessels in vitro quantitatively mirrored that of native vessels in vivo. Computational analysis indicated that cAMP stabilized vessels partly via its enhancement of barrier function.


Journal of Biomedical Materials Research Part A | 2013

Artificial lymphatic drainage systems for vascularized microfluidic scaffolds.

Keith H. K. Wong; James G. Truslow; Aimal H. Khankhel; Kelvin L. S. Chan; Joe Tien

The formation of a stably perfused microvasculature continues to be a major challenge in tissue engineering. Previous work has suggested the importance of a sufficiently large transmural pressure in maintaining vascular stability and perfusion. Here we show that a system of empty channels that provides a drainage function analogous to that of lymphatic microvasculature in vivo can stabilize vascular adhesion and maintain perfusion rate in dense, hydraulically resistive fibrin scaffolds in vitro. In the absence of drainage, endothelial delamination increased as scaffold density increased from 6 to 30 mg/mL and scaffold hydraulic conductivity decreased by a factor of 20. Single drainage channels exerted only localized vascular stabilization, the extent of which depended on the distance between vessel and drainage as well as scaffold density. Computational modeling of these experiments yielded an estimate of 0.40-1.36 cm H2O for the minimum transmural pressure required for vascular stability. We further designed and constructed fibrin patches (0.8 × 0.9 cm(2)) that were perfused by a parallel array of vessels and drained by an orthogonal array of drainage channels; only with the drainage did the vessels display long-term stability and perfusion. This work underscores the importance of drainage in vascularization, especially when a dense, hydraulically resistive scaffold is used.


Journal of the American Chemical Society | 2008

Bonding of macromolecular hydrogels using perturbants.

Gavrielle M. Price; Kengyeh K. Chu; James G. Truslow; Min D. Tang-Schomer; Andrew P. Golden; Jerome Mertz; Joe Tien

This work describes a method to bond patterned macromolecular gels into monolithic structures using perturbants. Bonding strengths for a variety of solutes follow a Hofmeister ordering; this result and optical measurements indicate that bonding occurs by reversible perturbation of contacting gels. The resulting microfluidic gels are mechanically robust and can serve as scaffolds for cell culture.


Biomaterials | 2009

Computational design of drainage systems for vascularized scaffolds

James G. Truslow; Gavrielle M. Price; Joe Tien

This computational study analyzes how to design a drainage system for porous scaffolds so that the scaffolds can be vascularized and perfused without collapse of the vessel lumens. We postulate that vascular transmural pressure--the difference between lumenal and interstitial pressures--must exceed a threshold value to avoid collapse. Model geometries consisted of hexagonal arrays of open channels in an isotropic scaffold, in which a small subset of channels was selected for drainage. Fluid flow through the vessels and drainage channel, across the vascular wall, and through the scaffold were governed by Navier-Stokes equations, Starlings Law of Filtration, and Darcys Law, respectively. We found that each drainage channel could maintain a threshold transmural pressure only in nearby vessels, with a radius-of-action dependent on vascular geometry and the hydraulic properties of the vascular wall and scaffold. We illustrate how these results can be applied to microvascular tissue engineering, and suggest that scaffolds be designed with both perfusion and drainage in mind.


PLOS ONE | 2012

Modulation of Invasive Phenotype by Interstitial Pressure-Driven Convection in Aggregates of Human Breast Cancer Cells

Joe Tien; James G. Truslow; Celeste M. Nelson

This paper reports the effect of elevated pressure on the invasive phenotype of patterned three-dimensional (3D) aggregates of MDA-MB-231 human breast cancer cells. We found that the directionality of the interstitial pressure profile altered the frequency of invasion by cells located at the surface of an aggregate. In particular, application of pressure at one end of an aggregate suppressed invasion at the opposite end. Experimental alteration of the configuration of cell aggregates and computational modeling of the resulting flow and solute concentration profiles revealed that elevated pressure inhibited invasion by altering the chemical composition of the interstitial fluid near the surface of the aggregate. Our data reveal a link between hydrostatic pressure, interstitial convection, and invasion.


Microvascular Research | 2013

Determination of vascular permeability coefficients under slow luminal filling.

James G. Truslow; Joe Tien

This Communication describes a method to obtain the permeability product (permeability coefficient normalized by vascular dimensions) from time-lapse intensity data for which the introduction of labeled solute into the vasculature does not occur at a sharply defined time. This method has an error of ~10% across a wide range of filling times and noise levels, and is particularly well-suited for situations in which the permeability coefficient is greater than 10(-6)cm/s. We show that it is applicable whether the increase in vascular solute concentration is sustained or transient.


Biomicrofluidics | 2011

Perfusion systems that minimize vascular volume fraction in engineered tissues

James G. Truslow; Joe Tien


Archive | 2014

Biophysical Mechanisms That Govern the Vascularization of Microfluidic Scaffolds

Keith H. K. Wong; James G. Truslow; Aimal H. Khankhel; Joe Tien

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