Network


Latest external collaboration on country level. Dive into details by clicking on the dots.

Hotspot


Dive into the research topics where Jamespandi Annaraj is active.

Publication


Featured researches published by Jamespandi Annaraj.


New Journal of Chemistry | 2017

Mixed-ligand copper(II) Schiff base complexes: the vital role of co-ligands in DNA/protein interactions and cytotoxicity

Sellamuthu Kathiresan; Subramanian Mugesh; Jamespandi Annaraj; Maruthamuthu Murugan

A series of four new mixed-ligand copper(II) complexes (1–4) of the type [Cu(L)(diimine)] (ClO4) [where L is 2-((1H-imidazol-2-yl)methylene)-N-phenylhydrazinecarbothioamide and the diimines are 1,10-phenanthroline (phen, 1), 2,2′-bipyridine (bpy, 2), 4,4′-dimethyl-2,2′-bipyridyl (dmbpy, 3), and 2,2′-dipyridylamine (dpa, 4)] have been successfully synthesized and characterized by various spectral techniques. The Kb values were calculated from electronic absorption spectral titration of these complexes with herring sperm DNA, and these varied in the order phen (1) > dmbpy (3) > bpy (2) > dpa (4). Electrophoresis observations revealed that these complexes (1–4) could efficiently induce single-strand breakage of pUC18 plasmid DNA in the presence of ascorbic acid. These copper complexes underwent a static quenching process with BSA. Moreover, their potential free-radical scavenging and anti-inflammatory properties were also determined using DPPH and protein denaturation techniques. These complexes showed efficient antibacterial activities against Staphylococcus aureus (Gram positive) and Pseudomonas aeruginosa (Gram negative). Furthermore, studies of their in vitro cytotoxicity against AGS cancer cells indicated promising antitumor activity with significant IC50 values.


RSC Advances | 2016

Mixed-ligand copper(II)-phenolate complexes: structure and studies on DNA/protein binding profiles, DNA cleavage, molecular docking and cytotoxicity

Sellamuthu Kathiresan; Subramanian Mugesh; Maruthamuthu Murugan; Feroze Ahamed; Jamespandi Annaraj

Copper(II) complexes with simple and mixed ligands, [Cu(L)(ClO4)] (1) and [Cu(L)(diimine)]ClO4 (2–4) [where L is 4-chloro-2-((2-(phenylthio)phenylimino)methyl)phenol and diimine is 1,10-phenanthroline (phen, 2), 2,2′-bipyridine (bpy, 3) or 4,4′-dimethyl-2,2′-bipyridyl (dmbpy, 4)], were synthesized and characterized by elemental analysis, UV-vis, FT-IR, electrospray ionization-mass spectrometry (ESI-MS) and electrochemical studies. Notably, complex 4 was structurally characterized using single X-ray crystallography. It was observed that this complex has a slightly distorted square planar geometry. The varying interactions of these complexes with herring sperm DNA (HS-DNA) were explored in detail using various spectral and electrochemical methods to gain insight into their structure–activity relationships. The obtained results revealed that complexes 1, 3 and 4 could interact with HS-DNA via a partial intercalation mode, whereas complex 2 was found to deeply stack between base pairs with a binding constant of 104 M−1 due to its enhanced planarity; moreover, 4 underwent a hydrophobic interaction with DNA. These experimental observations were found to be close to the theoretical observations investigated by the molecular docking technique. The interaction of these synthesized Cu(II) complexes with bovine serum albumin (BSA) was also evaluated using absorption and fluorescence techniques, which revealed a static quenching mechanism between the complexes and BSA. In addition, DNA cleavage by the complexes was monitored by electrophoretic spectrometry; the results showed that these complexes exhibited significant cleavage in the presence of a reducing agent (ascorbic acid). The in vitro cytotoxicity of these Cu(II) complexes was carried out in two different human tumour cell lines, A549 and Huh7. Furthermore, molecular docking was also used to evaluate and understand the interaction modes of the complexes with the molecular target DNA. All the in vitro pharmacological evaluation observations clearly indicated the superior DNA binding/cleaving and protein binding properties of these complexes, although the S-donor atom did not coordinate with the central copper ion in the mixed complexes.


Journal of Materials Chemistry B | 2017

A Schiff's base receptor for red fluorescence live cell imaging of Zn2+ ions in zebrafish embryos and naked eye detection of Ni2+ ions for bio-analytical applications

A. Senthil Murugan; N. Vidhyalakshmi; U. Ramesh; Jamespandi Annaraj

An interesting dual chemoreceptor (QAMP) was synthesized to quantify the presence of two environmentally as well as biologically important Zn2+/Ni2+ ions in a highly selective manner using different analytical techniques. The fluorescence profile was enhanced to 663 nm (red region) due to the formation of its 1 : 1 complex with Zn2+ at room temperature even in the presence of other interfering ions such as Cd2+ and Hg2+. Moreover, it exhibits excellent chromo-isomerism with Ni2+ ions. Meanwhile, this assay could be successfully oriented with molecular logic functions of AND, OR, NOR and NOT logic gates. The obtained large Stokes shift (∼274 nm) value and red emission promoted this receptor as a potential tool for biological studies (e.g. live cell imaging in cancer cell line and zebrafish models).


Journal of Coordination Chemistry | 2016

Biological evaluation of redox stable cisplatin/Cu(II)-DNA adducts as potential anticancer agents

Sellamuthu Kathiresan; Raman Dhivya; Murugesan Vigneshwar; Marichamy Rajasekaran; Jyothi Ranjani; Jeyaprakash Rajendhran; Sankaran Srinivasan; Subramanian Mugesh; Maruthamuthu Murugan; Periakaruppan Athappan; Jamespandi Annaraj

Abstract A series of non-enolizable β-diketonate-based copper(II) complexes with LCuCl2 [L = Knoevenagel condensates of curcumin (Salcimine) and methylacetoacetate (SalMaA)-based Schiff bases] chromospheres as functional models of chemotherapy drug cisplatin were investigated for their covalent interaction with herring sperm DNA. The synthesis and structural characterization of 1a and 1b have been reported in our previous article. However, their DNA interactions and cytotoxicity properties were not studied. These analyses have been carried out mainly through electrochemical techniques supplemented with spectral, relative viscosity, gel electrophoresis techniques, and AGS cancer cells using MTT assay. The cytotoxic activities of the ligand, curcumin-based copper complex, and cisplatin were tested against the AGS cancer cell line under similar experimental conditions showing that the complex exhibited cancer cell inhibitory rate closer to cisplatin even at low concentration. This was also seen in the docking of the Cu-complex onto a rich guanine B-DNA decamer, where a Cu–N3(guanine) interaction instead of Pt-N7 as cisplatin is detected. The obtained results in this study prove that these complexes could be a promising substitute for cisplatin as a new family of non-platinum-based anticancer metallo-drugs after in vivo tests on animal models.


Journal of Photochemistry and Photobiology B-biology | 2015

Synthesis, spectral characterization and DNA bindings of tridentate N2O donor Schiff base metal(II) complexes.

Sellamuthu Kathiresan; Thangavel Anand; Subramanian Mugesh; Jamespandi Annaraj

To evaluate the biological preference of synthetic small drugs towards DNA target, new metal based chemotherapeutic agents of Cu(II), Co(II), Ni(II) and Zn(II), 2,4-diiodo-6-((pyridin-2-ylmethylimino)methyl)phenol (L) Schiff base complexes (1, 2, 3 &4) having N,N,O donor system respectively were synthesized and thoroughly characterized. The IR results confirmed the tridentate binding of the ligand with metal centre during complexation and reflects the proposed structure. The density function theory calculations were also used to further investigate the electronic structure and properties of ligand and complexes. The preliminary investigation of herring Sperm (HS-DNA) interaction propensity of complexes 1-4 were carried out in Tris-HCl buffer at pH 7.1 to demonstrate their mode of interactions. The obtained results reveal that these complexes significantly interact with DNA on the grooves, further, this observed mode of interactions was also confirmed by molecular docking evaluations. The complexes 1-4 were also screened for antimicrobial evaluations which demonstrated that their significant activity against various human pathogens. The cleavage studies with pBR322 plasmid DNA revealed higher nuclease activity of 1 as compared to other complexes.


Materials Science and Engineering: C | 2017

Biocompatible curcumin loaded PMMA-PEG/ZnO nanocomposite induce apoptosis and cytotoxicity in human gastric cancer cells

Raman Dhivya; Jothi Ranjani; Patrick K. Bowen; Jeyaprakash Rajendhran; Jeyanthinath Mayandi; Jamespandi Annaraj

Although curcumin is efficient in killing cancer cells, its poor water solubility and assocaited inadequate bioavailability remain major limitations to its therapeutic application. The formulation of curcumin micellar nanoparticles (NPs) encapsulated with a biodegradable polymer promises to significantly improve curcumins solubility, stability, and bioavailability. The past decade has witnessed the development of nanoscale curcumin delivery systems: curcumin-loaded liposomes or nanoparticles, self-microemulsifying drug delivery systems (SMEDDS), cyclodextrin inclusions, solid dispersions, nanodisks, and nanotubes. The intention of the present investigation was to enhance the bioavailability and ultimately the efficacy of curcumin by developing a curcumin loaded PMMA-PEG/ZnO bionanocomposite utilizing insoluble curcumin and poorly soluble ZnO nanoparticles. Here, the drug (curcumin) may be carry and deliver the biomolecule(s) by polymer-encapsulated ZnO NPs. Physical characteristics of these novel nanomaterials have been studied with transmission electron microscopy (TEM) and powder X-ray diffraction (XRD) in conjunction with spectral techniques. Aqueous solubility of curcumin was augmented upon conjugation with the polymer-stabilized ZnO NPs. A narrow nanocomposite particle size distribution with an average value of 40 to 90nm was found via TEM. Most importantly, the pH-responsive release of curcumin from the nano-vehicle ensures safer, more controlled delivery of the drug at physiological pH. Cytotoxic potential and cellular uptake of curcumin loaded ZnO NPs were assessed by) cell viability assay, cell cycle assays along with the cell imaging studies have been done in addition to MTT using AGS cancer cells. Hence, these studies demonstrate that the clinical potential of the Curcumin Loaded PMMA-PEG/ZnO can induce the apoptosis of cancer cells through a cell cycle mediated apoptosis corridor, which raises its probability to cure gastric cancer cells.


Materials Science and Engineering: C | 2018

Enhancing the anti-gastric cancer activity of curcumin with biocompatible and pH sensitive PMMA-AA/ZnO nanoparticles

Raman Dhivya; Jothi Ranjani; Jeyaprakash Rajendhran; Jeyanthinath Mayandi; Jamespandi Annaraj

Curcumin loaded ZnO nanoparticles were successfully synthesised and encapsulated with co-polymer PMMA-AA (Cur/PMMA-AA/ZnO NPs). The ZnO nanoparticles have been converted as good cargo materials to carry the well-known hydrophobic drug curcumin by surface functionalization. Physical characteristics of these novel nanomaterials have been studied with transmission electron microscopy (TEM) and powder X-ray diffraction (XRD) in conjunction with spectral techniques. A narrow particle size distribution with an average value of 42nm was found via TEM. Most importantly, the pH-responsive release of curcumin from the nano-vehicle ensures safer, more controlled delivery of the drug at physiological pH. The drug entrapment efficiency and loading was evaluated and the in vitro efficacy as anticancer drug delivery vehicle was analyzed. The potential toxicity of Cur/PMMA-AA/ZnO NPs was studied by using AGS gastric cancer cell lines via MTT assay. These results revealed that the proposed nanomaterials induce a remarkable cell death in in-vitro models. The multifunctional properties of Cur/PMMA-AA/ZnO NPs may open up new avenues in cancer therapy through overcoming the limitations of conventional cancer therapy.


Journal of Biomedical Materials Research Part A | 2018

Magnetic iron oxide nanoparticles (MIONs) cross-linked natural polymer-based hybrid gel beads: Controlled nano anti-TB drug delivery application: CONTROLLED NANO ANTI-TB DRUG DELIVERY APPLICATION

Mookkandi Palsamy Kesavan; Srinivasan Ayyanaar; V. Vijayakumar; Jeyaraj Dhaveethu Raja; Jamespandi Annaraj; Kathiresan Sakthipandi; Jegathalaprathaban Rajesh

The nanosized rifampicin (RIF) has been prepared to increase the solubility in aqueous solution, which leads to remarkable enhancement of its bioavailability and their convenient delivery system studied by newly produced nontoxic, biodegradable magnetic iron oxide nanoparticles (MIONs) cross-linked polyethylene glycol hybrid chitosan (mCS-PEG) gel beads. The functionalization of both nano RIF and mCS-PEG gel beads were studied using various spectroscopic and microscopic techniques. The size of prepared nano RIF was found to be 70.20 ± 3.50 nm. The mechanical stability and swelling ratio of the magnetic gel beads increased by the addition of PEG with a maximum swelling ratio of 38.67 ± 0.29 g/g. Interestingly, this magnetic gel bead has dual responsive assets in the nano drug delivery application (pH and the magnetic field). As we expected, magnetic gel beads show higher nano drug releasing efficacy at acidic medium (pH = 5.0) with maximum efficiency of 71.00 ± 0.87%. This efficacy may also be tuned by altering the external magnetic field and the weight percentage (wt%) of PEG. These results suggest that such a dual responsive magnetic gel beads can be used as a potential system in the nano drug delivery applications.


Journal of Nanomaterials | 2014

Synthesis, characterization, and DNA binding studies of nanoplumbagin

Sheik Dawood Shahida Parveen; Abdullah Affrose; Basuvaraj Suresh Kumar; Jamespandi Annaraj; Kasi Pitchumani

The traditional anticancer medicine plumbagin (PLN) was prepared as nanostructured material (nanoplumbagin, NPn1) from its commercial counterparts, simultaneously coencapsulating with cetyltrimethylammonium bromide or cyclodextrin as stabilizers using ultrasonication technique. Surface morphology of NPn analysed from atomic force microscopy (AFM) indicates that NPn has tunable size between 75 nm and 100 nm with narrow particle size distribution. Its binding efficiency with herring sperm DNA was studied using spectral and electrochemical techniques and its efficiency was found to be more compared to the commercial microcrystalline plumbagin (PLN). DNA cleavage was also studied by gel electrophoresis. The observed results indicate that NPn1 has better solubility in aqueous medium and hence showed better bioavailability compared to its commercial counterparts.


Journal of Inorganic Biochemistry | 2005

Spectral and redox studies on mixed ligand complexes of cobalt(III) phenanthroline/bipyridyl and benzoylhydrazones, their DNA binding and antimicrobial activity

S. Srinivasan; Jamespandi Annaraj; Periakaruppan Athappan

Collaboration


Dive into the Jamespandi Annaraj's collaboration.

Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar

N. Vidhyalakshmi

Madurai Kamaraj University

View shared research outputs
Top Co-Authors

Avatar

Raman Dhivya

Madurai Kamaraj University

View shared research outputs
Top Co-Authors

Avatar
Top Co-Authors

Avatar

U. Ramesh

Madurai Kamaraj University

View shared research outputs
Top Co-Authors

Avatar
Top Co-Authors

Avatar

Jothi Ranjani

Madurai Kamaraj University

View shared research outputs
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Researchain Logo
Decentralizing Knowledge