Jang-Sun Suh

Breeding progress and characterization of a Korean white variety `Baek-A` in Flammulina velutipes

We made the first Korean white commercial strain `Baek-a` developed by crossing between monokaryons derived from brown strains. This variety can be estimated as the Korea`s indigenous one different from the origin of Japanese white ones. The optimum temperature of mycelial growth was but it needed to adjust to when incubated at the bottle cultivation. The optimum temperatures of fruiting body initiation and development were and , respectively. Fruiting body of `Baek-a` was pure white even developed from crossing with brown strains. `Baek-a` was a good variety with high quality and high productivity characterized as quite even budding habit, long stipes and hemi-spherical pilei. The days for the fruiting was 7 days and the productivity was g per 850 ml. This variety needed high concentration of carbon dioxide and it had to be adjusted up to 4,000 ppm for the good quality.

Pseudomonas azotoformans HC5 Effective in Antagonistic of Mushrooms Brown Blotch Disease Caused by Pseudomonas tolaasii

A gram-negative bacterium was isolated from spent substrate of Agaricus bisporus and showed marked antagonistic activity against Pseudomonas tolaasii. The bacterium was identified as Pseudomonas azotoformans by based on the cultural, biochemical and physiological characteristics, and 16S rRNA gene sequence. The isolated bacterium was saprophytic but not parasitic nor pathogenic to cultivation mushroom. The isolated bacterium for P. tolaasii cell was not sufficient for inhibition in vitro. Control efficacy of Pseudomonas azotoformans HC5 to brown blotch of P. tolaasii was 73, 78, and 71% on A. bisporus, Flammulina velutipes, and Pleurotus ostreatus, respectively. In the future, the suppressive bacterium may be useful for development of a biocontrol system.

Comparison of amino acid and free amino acid contents between cap and stipe in Flammulina velutipes and Pleurotus ostreatus

팽이버섯과 느타리버섯의 색깔이 다른 계통과 부위별로 갓과 대로 나누어 아미노산 및 유리아미노산 분석을 실시하여 성분 조성과 함량을 비교하였다. 그 결과 버섯 종류와 계통, 그리고 부위에 따라 서로 다른 아미노산 종류가 검출되었다. 팽이, 느타리 모두에서 tryptophane은 검출되지 않았으며, 느타리에서는 특이적으로 alanine이 검출되지 않았다. 또한 팽이에서는 glutamic acid 함량이 가장 높았으며 methionine과 phenylalanine은 극히 적게 검출되었다. 느타리버섯에서는 glutamic acid 함량이 가장 높았고 phenylalanine과 cysteine이 낮았으며 특이하게 흑색변이계통에서 leucine이 검출되지 않았다. 또한 부위별로는 팽이와 느타리 모두 아미노산 종류별 비율은 동일한 경향을 보였으며, 일반적으로 대보다는 갓에서 아미노산 함량이 더 높은 경향을 보였다. 유리아미노산 분석 결과에서는 버섯계통별로 검출되는 아미노산 함량이 서로 달랐으며, 버섯의 갓과 대에서도 검출되는 아미노산 조성이 일치하지 않는 것으로 나타났다. 【The composition and content of amino acid and free amino acid were separately compared in cap and stipe of fruitbody obtained from different color strains in winter mushroom and oyster mushroom strains. The result showed different kinds and amount of amino acids according to the mushroom parts, strains and kinds. Tryptophane was not detected in any mushrooms. Alanine was also not detected in all oyster mushroom strains. The content of glutamic acid was the highest and that of methionine, phenylalanine and cysteine were very low in all mushroom strains. When compared by parts, the ratio of amono acids showed similar tendency in composition. Generally the content of amono acids showed higher in caps than in stipes. The profile and content of free amino acid showed a lot of variation between cap and strip in the intra and inter mushrooms.】

Effects of microorganism density and mushroom yields according to the sterilization of casing soils at the cultivation of button mushrooms

양송이 재배농가는 복토재료로 흙을 사용하고 있으며, 대부분의 농가는 채취된 그대로 사용하고 있어 흙속에 있는 각종 병원균의 감염으로 버섯재배에 실패하는 농가가 많다. 복토 살균온도에 따른 미생물의 밀도변화를 조사한 결과 호기성세균은 양송이 생육주기가 길어질수록 증가하였으며 2주기 수확후 부터는 급격히 증가하였다. 사상균은 복토 8일째 무처리에서 급격히 증가하였고, 살균 처리구에서는 증감을 반복하다가 2주기 수확후 부터 급격히 증가하였다. 푸른곰팡이균도 증감을 반복하다가 2주기 수확후부터 급격히 증가하였다. 형광성 Psuedomonas속은 살균온도가 높은 처리구에서 높은 밀도를 보였으며, 복토 22일에는

Growth characteristics and productivity of oyster mushroom according to adding of Rhus verniciflua sawdust

100^{circ}C

Phylegenetic analysis of Hericium species based on ITS rDNA sequences

-30분과 60분에서 다른 처리구보다 급격히 증가하였다. 중온성 방선균은 복토 15일 약간 증가하는 경향을 보였다가 1주기 수확후부터 급격히 증가하였다. 고온성 방선균은 복토 15일에 급격히 증가하여 복토 22일에 가장 높은 밀도를 보였지만, 1주기 수확후 부터 급격히 감소하였다. 양송이버섯의 수량과 품질은

Cultivation conditions for mass production of detoxifying bacterium Pseudomonas sp. HC1 of tolaasin produced by Pseudomonas tolaasii

80^{circ}C

Characteristics of Culture of Oyster Mushroom (Pleurotus ostreatus) on Addition Rate of Acer tegmentosum

-90분에서는 상품 및 중품의 수량이 가장 높았다. 병해충 발생은

Characteristics and breeding of a new multi-generation oyster mushroom (Pleurotus ostreatus) variety 'Dagul'

100^{circ}C

Quality standard of Agaricus bisporus in a market and changes of mushroom quality during storage

-30분 처리에서 가장 낮았지만 양송이의 수량이 낮았으며,