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Dive into the research topics where Jayne Wu is active.

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Featured researches published by Jayne Wu.


Biosensors and Bioelectronics | 2017

A highly sensitive and specific capacitive aptasensor for rapid and label-free trace analysis of Bisphenol A (BPA) in canned foods.

Hadi Mirzajani; Cheng Cheng; Jayne Wu; Jiangang Chen; Shigotoshi Eda; Esmaeil Najafi Aghdam; Habib Badri Ghavifekr

A rapid, highly sensitive, specific and low-cost capacitive affinity biosensor is presented here for label-free and single step detection of Bisphenol A (BPA). The sensor design allows rapid prototyping at low-cost using printed circuit board material by benchtop equipment. High sensitivity detection is achieved through the use of a BPA-specific aptamer as probe molecule and large electrodes to enhance AC-electroelectrothermal effect for long-range transport of BPA molecules toward electrode surface. Capacitive sensing technique is used to determine the bounded BPA level by measuring the sample/electrode interfacial capacitance of the sensor. The developed biosensor can detect BPA level in 20s and exhibits a large linear range from 1 fM to 10 pM, with a limit of detection (LOD) of 152.93 aM. This biosensor was applied to test BPA in canned food samples and could successfully recover the levels of spiked BPA. This sensor technology is demonstrated to be highly promising and reliable for rapid, sensitive and on-site monitoring of BPA in food samples.


ACS Applied Materials & Interfaces | 2016

Bisphenol A Sensors on Polyimide Fabricated by Laser Direct Writing for Onsite River Water Monitoring at Attomolar Concentration

Cheng Cheng; Shutong Wang; Jayne Wu; Yongchao Yu; Ruozhou Li; Shigetoshi Eda; Jiangang Chen; Guoying Feng; Benjamin Lawrie; Anming Hu

This work presents an aptamer-based, highly sensitive and specific sensor for atto- to femtomolar level detection of bisphenol A (BPA). Because of its widespread use in numerous products, BPA enters surface water from effluent discharges during its manufacture, use, and from waste landfill sites throughout the world. On-site measurement of BPA concentrations in water is important for evaluating compliance with water quality standards or environmental risk levels of the harmful compound in the environment. The sensor in this work is porous, conducting, interdigitated electrodes that are formed by laser-induced carbonization of flexible polyimide sheets. BPA-specific aptamer is immobilized on the electrodes as the probe, and its binding with BPA at the electrode surface is detected by capacitive sensing. The binding process is aided by ac electroosmotic effect that accelerates the transport of BPA molecules to the nanoporous graphene-like structured electrodes. The sensor achieved a limit of detection of 58.28 aM with a response time of 20 s. The sensor is further applied for recovery analysis of BPA spiked in surface water. This work provides an affordable platform for highly sensitive, real time, and field-deployable BPA surveillance critical to the evaluation of the ecological impact of BPA exposure.


Biosensors and Bioelectronics | 2017

Rapid and sensitive detection of bisphenol a from serum matrix

Xiaogang Lin; Cheng Cheng; Paul Terry; Jiangang Chen; Haochen Cui; Jayne Wu

Bisphenol A (BPA) is an endocrine disrupting compound that may have adverse developmental, reproductive, neurological, and immune system effects. Low-level exposure to BPA is ubiquitous in human populations due to its widespread use in consumer products. Therefore, highly sensitive methods are needed to quantify BPA in various matrices including water, serum, and food products. In this study, we developed a simple, rapid, highly sensitive and specific sensor based on an aptamer probe and AC electrokinetics capacitive sensing method that successfully detected BPA at femto molar (fM) levels, which is an improvement over prior work by a factor of 10. We were able to detect BPA spiked in human serum as well as in maternal and cord blood within 30s. The sensor is responsive to BPA down to femto molar levels, but not to structurally similar compounds including bisphenol F (BPF) or bisphenol S (BPS) even at much higher concentration. Further development of this platform may prove useful in monitoring exposure to BPA and other small molecules in various matrices.


Mikrochimica Acta | 2015

Rapid capacitive detection of femtomolar levels of bisphenol A using an aptamer-modified disposable microelectrode array

Haochen Cui; Jayne Wu; Shigetoshi Eda; Jiangang Chen; Wei Chen; Lei Zheng

AbstractA label-free and single-step method is reported for rapid and highly sensitive detection of bisphenol A (BPA) in aqueous samples. It utilizes an aptamerxa0acting asxa0a probe molecule immobilized on a commercially available array of interdigitated aluminum microelectrodes. BPA was quantified by measuring the interfacial capacitance change rate caused by the specific binding between bisphenol A and the immobilized aptamer. The AC signal also inducesxa0an AC electrokinetic effect to generate microfluidic motion for enhanced binding. The capacitive aptasensor achieves a limit of detection as low as 10xa0fM(2.8xa0fgu2009⋅u2009mL−u20091) with a 20xa0s response time. The method is inexpensive, highly sensitive, rapid and therefore provides a promising technology for on-site detection of BPA in food and water samples.n Graphical AbstractA. AC electrokinetics effect plays a vital role in BPA detection by introducing microfluidic movement to accelerate the molecular transport to the electrode surface.n B. The ACEK capacitive aptasensor has a limit of detection as low as 10 fM (2.8xa0fgu2009⋅u2009mL−u20091) with a 20-s response time.n


Biosensors and Bioelectronics | 2017

A low cost and palm-size analyzer for rapid and sensitive protein detection by AC electrokinetics capacitive sensing

Xiaozhu Liu; Cheng Cheng; Jayne Wu; Shigetoshi Eda; Yongcai Guo

Specific detection of protein biomarkers has a wide range of applications in areas such as medical science, diagnostics, and pharmacology. Quantitative detection of protein biomarkers in biological media, such as serum, is critically important in detecting disease or physiological malfunction, or tracking disease progression. Among various detection methods, electrical detection is particularly well suited for point-of-care (POC) specific protein detection, being of low cost, light weight and small form factor. A portable system for sensitive and quantitative detection of protein biomarkers will be highly valuable in controlling and preventing diseases outbreaks. Recently, an alternating current electrokinetic (ACEK) capacitive sensing method has been reported to demonstrate very promising performance on rapid and sensitive detection of specific protein from serum. In this work, a low cost and portable analyzer with good accuracy is developed to use with ACEK capacitive sensing to produce a true POC technology. The development of a board-level capacitance readout system is presented, as well as the adaption of the protocol for use with ACEK capacitive sensing. Results showed that the developed system could achieve a limit of detection of 10ng/mL, comparable to a sophisticated benchtop instrument. With its small size and light-weight similar to a smart phone, the developed system is ready to be applicable to POC diagnostics. Further, the readout system can be readily expanded for multichannel monitoring and telecommunication capabilities.


ieee sensors | 2013

Rapid detection of progesterone by commercially available microelectrode chips

Haochen Cui; Cheng Cheng; Jayne Wu; Shigetoshi Eda

This work presents rapid detection of progesterone by utilizing a commercially available surface acoustic wave (SAW) resonator chip in coordination with an impedance affinity sensing method developed in our group. Interdigitated microelectrodes from the SAW chips were coated with anti-progesterone polyclonal antibody to capture progesterone and subsequently exposed to a solution containing progesterone. With the help of alternating current electrokinetics (ACEK) effects, binding of progesterone to the immobilized antibody was accelerated and, within two minutes, could be detected by continuous reading of the interfacial capacitance change over the microelectrodes. This ACEK-based capacitive sensor is of low cost, highly portable and easy to use, makes it perfect for point-of-care applications.


Mikrochimica Acta | 2017

A PCR-free point-of-care capacitive immunoassay for influenza A virus

Cheng Cheng; Haochen Cui; Jayne Wu; Shigetoshi Eda

AbstractThis article describes a highly sensitive and specific capacitive immunosensor for rapid, low cost and simple-to-use detection of virus particles from clinical swab samples. An inhomogeneous AC electric field is applied on sensor electrodes. This induces positive dielectrophoresis that attracts virus particles to the sensor electrodes. As a result, rapid and sensitive detection of influenza A virus is accomplished without the need for nucleotide isolation and amplification. The same AC signal is used to detect the binding of virus particle to the sensor surface immobilized with the antibody probe. The assay is highly suitable for point-of-care use. When testing clinical swab samples, the response of samples at various dilutions is analyzed, and an optimal dilution is found and used for subsequent blind tests of clinical swab samples. Analytical experiments on standard influenza virus sample demonstrate a limit of detection of 0.25xa0pg⋅mL−1. Other figures of merit include (a) an assay time of 30xa0seconds; (b) a diagnostic sensitivity of 90%; and (c) a specificity of 70%. Blind tests are conducted for a panel of twenty nasal swab samples, and the results are in good agreement with those by using the commercial reverse transcription polymerase chain reaction.n Graphical AbstractA low AC signal is applied onto an interdigitated electrode sensor, inducing dielectrophoresis for virus enrichment with simultaneous interfacial capacitance sensing. Rapid (30xa0seconds) detection is achieved with a sensitivity of 90% and specificity of 70%, compared against RT-PCR results.


Electrophoresis | 2017

Capacitive DNA sensor for rapid and sensitive detection of whole genome human herpesvirus-1 dsDNA in serum

Cheng Cheng; Rania Oueslati; Jayne Wu; Jiangang Chen; Shigetoshi Eda

This work presents a rapid, highly sensitive, low‐cost, and specific capacitive DNA sensor for detection of whole genome human herpesvirus‐1 DNA. This sensor is capable of direct DNA detection with a response time of 30 s, and it can be used to test standard buffer or serum samples. The sensing approach for DNA detection is based on alternating current (AC) electrokinetics. By applying an inhomogeneous AC electric field on sensor electrodes, positive dielectrophoresis is induced to accelerate DNA hybridization. The same applied AC signal also directly measures the hybridization of target with the probe on the sensor surface. Experiments are conducted to optimize the AC signal, as well as the buffers for probe immobilization and target DNA hybridization. The assay is highly sensitive and specific, with no response to human herpesvirus‐2 DNA at 5 ng/mL and a LOD of 1.0 pg/mL (6.5 copies/μL or 10.7 aM) in standard buffer. When testing the double stranded (ds) DNA spiked in human serum samples, the sensor yields a LOD of 20.0 pg/mL (129.5 copies/μL or 0.21 femtomolar (fM)) in neat serum. In this work, the target is whole genome dsDNA, consequently the test can be performed without the use of enzyme or amplification, which considerably simplifies the sensor operation and is highly suitable for point of care disease diagnosis.


Biosensors and Bioelectronics | 2018

Highly sensitive and specific on-site detection of serum cocaine by a low cost aptasensor

Rania Oueslati; Cheng Cheng; Jayne Wu; Jiangang Chen

Cocaine is one of the most used illegal recreational drugs. Developing an on-site test for cocaine use detection has been a focus of research effort, since it is essential to the control and legal action against drug abuse. Currently most of cocaine detection methods are time-consuming and require special or expensive equipment, and the detection often suffers from high cross-reactivity with cocaine metabolites and relative low sensitivity with the best limit of detection reported at sub nanomolar (nM) level. In this work, an aptasensor has been developed using capacitive monitoring of sensor surface incorporating alternating current electrokinetics effects to speed up molecular transport and minimize matrix effects. The aptasensor is rapid, low cost, highly sensitive and specific as well as simple-to-use for the detection of cocaine from serum. The assay has a sample-to-result time of 30u202fs, a limit of detection of 7.8 fM, and a linear response for cocaine ranging from 14.5fM to 14.5pM in standard buffer, which are great improvements from other reported cocaine sensors. Special buffer is used for serum cocaine detection, and a limit of detection of 13.4 fM is experimentally demonstrated for cocaine spiked in human serum (equivalent to 1.34pM cocaine in neat serum). The specificity of the biosensor is also demonstrated with structurally similar chemicals, ecgonine ethyl ester and methylecgonidine. This biosensor shows high promise in detection of low levels of cocaine from complex matrices.


Biosensors and Bioelectronics | 2018

Rapid, highly sensitive detection of Gram-negative bacteria with lipopolysaccharide based disposable aptasensor

Jian Zhang; Rania Oueslati; Cheng Cheng; Ling Zhao; Jiangang Chen; Raul A. Almeida; Jayne Wu

Gram-negative bacteria are one of the most common microorganisms in the environment. Their differential detection and recognition from Gram-positive bacteria has been attracting much attention over the years. Using Escherichia coli (E. coli) as a model, we demonstrated on-site detection of Gram-negative bacteria by an AC electrokinetics-based capacitive sensing method using commercial microelectrodes functionalized with an aptamer specific to lipopolysaccharides. Dielectrophoresis effect was utilized to enrich viable bacteria to the microelectrodes rapidly, achieving a detection limit of 102 cells/mL within a 30u202fs response time. The sensor showed a negligible response to Staphylococcus aureus (S. aureus), a Gram-positive species. The developed sensor showed significant advantages in sensitivity, selectivity, cost, operation simplicity, and response time. Therefore, this sensing method has shown great application potential for environmental monitoring, food safety, and real-time diagnosis.

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Cheng Cheng

University of Tennessee

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Haochen Cui

University of Tennessee

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Anming Hu

University of Tennessee

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Paul Terry

University of Tennessee

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Quan Yuan

University of Tennessee

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