Jean Baptiste Galey

The anti-ageing potential of a new jasmonic acid derivative (LR2412): in vitro evaluation using reconstructed epidermis episkin™

Abstract:  Jasmonic acid is involved in plant wound repair and tissue regeneration, but no study has been reported in human skin. The effect of a jasmonic acid derivative, tetra‐hydro‐jasmonic acid (LR2412, 1 and 10 μm) was investigated on an in vitro reconstructed skin model, Episkin™. Using real time RTQPCR studies, results showed an increase in hyaluronan synthase 2 (HAS2) and hyaluronase synthase 3 (HAS3) expression. Furthermore, an increase in hyaluronic acid (HA) deposits in basal and suprabasal layers of the epidermis was observed. The percentage of positive Ki67 keratinocytes in the basal layer as well as the epidermis thickness were seen to increase. Immunohistochemistry studies showed that the synthesis of late differentiation proteins filaggrin and transglutaminase 1 was not modified. The human epidermis is known to thin with age while HA content has been reported to decrease. These results illustrate the potential of LR2412 in counteracting signs of skin ageing.

Iron chelation can modulate UVA-induced lipid peroxidation and ferritin expression in human reconstructed epidermis.

Background/Purpose: As ferritin has been identified as an important factor in antioxidant defense in cultured human skin cells, we evaluated UVA‐induced lipid hydroperoxides (LPO) production and ferritin expression in reconstructed human epidermis in vitro.