Jean-François Arnal
Academy of Sciences of the Czech Republic
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Publication
Featured researches published by Jean-François Arnal.
Nucleic Acids Research | 2012
Henrik Laurell; Jason S. Iacovoni; Anne Abot; David Svec; Jean-José Maoret; Jean-François Arnal; Mikael Kubista
Genomic DNA (gDNA) contamination is an inherent problem during RNA purification that can lead to non-specific amplification and aberrant results in reverse transcription quantitative PCR (RT—qPCR). Currently, there is no alternative to RT(−) controls to evaluate the impact of the gDNA background on RT–PCR data. We propose a novel method (ValidPrime) that is more accurate than traditional RT(−) controls to test qPCR assays with respect to their sensitivity toward gDNA. ValidPrime measures the gDNA contribution using an optimized gDNA-specific ValidPrime assay (VPA) and gDNA reference sample(s). The VPA, targeting a non-transcribed locus, is used to measure the gDNA contents in RT(+) samples and the gDNA reference is used to normalize for GOI-specific differences in gDNA sensitivity. We demonstrate that the RNA-derived component of the signal can be accurately estimated and deduced from the total signal. ValidPrime corrects with high precision for both exogenous (spiked) and endogenous gDNA, contributing ∼60% of the total signal, whereas substantially reducing the number of required qPCR control reactions. In conclusion, ValidPrime offers a cost-efficient alternative to RT(−) controls and accurately corrects for signals derived from gDNA in RT–qPCR.
Archive | 2004
Hervé Prats; Henrik Laurell; Carine Bossard; Barbara Garmy-Susini; Jean-François Arnal
Archive | 2018
Eva Feigerlova; Henrik Laurell; Hervé Mittre; Marie-Laure Kottler; Marc Deshayes; Patrick Balaguer; William Bourget; Jean-François Arnal; Richard Marechaud; Samy Hadjadj; Pierre Gourdy
55th Annual ESPE | 2016
Eva Feigerlova; Henrik Laurell; Hervé Mittre; Marie-Laure Kottler; Marc Deshayes; Patrick Balaguyer; Jean-François Arnal; Richard Marechaud; Samy Hadjadj; Pierre Gourdy
17th European Congress of Endocrinology | 2015
Alexia Vinel; Coralie Fontaine; Eric Hay; Marie Cécile Valera; Françoise Lenfant; Martine Cohen-Solal; Jean-François Arnal
/data/revues/00034266/v74i2/S0003426613000280/ | 2013
Jean-François Arnal; Pierre Gourdy; Françoise Lenfant
Archive | 2009
Elodie Riant; Aurélie Waget; Haude Cogo; Jean-François Arnal; Rémy Burcelin; Pierre Gourdy
Reproduction humaine et hormones | 2007
Pierre Gourdy; Jean-François Arnal
Reproduction humaine et hormones | 2004
Jean-François Arnal; Pierre Gourdy; Barbara Garmy-Susini; Laurent Brouchet; Francis Bayard
Archive | 2004
Hervé Prats; Henrik Laurell; Carine Bossard; Barbara Garmy-Susini; Jean-François Arnal