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Jean-François Arnal

Academy of Sciences of the Czech Republic

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Nucleic Acids Research | 2012

Correction of RT–qPCR data for genomic DNA-derived signals with ValidPrime

Henrik Laurell; Jason S. Iacovoni; Anne Abot; David Svec; Jean-José Maoret; Jean-François Arnal; Mikael Kubista

Genomic DNA (gDNA) contamination is an inherent problem during RNA purification that can lead to non-specific amplification and aberrant results in reverse transcription quantitative PCR (RT—qPCR). Currently, there is no alternative to RT(−) controls to evaluate the impact of the gDNA background on RT–PCR data. We propose a novel method (ValidPrime) that is more accurate than traditional RT(−) controls to test qPCR assays with respect to their sensitivity toward gDNA. ValidPrime measures the gDNA contribution using an optimized gDNA-specific ValidPrime assay (VPA) and gDNA reference sample(s). The VPA, targeting a non-transcribed locus, is used to measure the gDNA contents in RT(+) samples and the gDNA reference is used to normalize for GOI-specific differences in gDNA sensitivity. We demonstrate that the RNA-derived component of the signal can be accurately estimated and deduced from the total signal. ValidPrime corrects with high precision for both exogenous (spiked) and endogenous gDNA, contributing ∼60% of the total signal, whereas substantially reducing the number of required qPCR control reactions. In conclusion, ValidPrime offers a cost-efficient alternative to RT(−) controls and accurately corrects for signals derived from gDNA in RT–qPCR.

Archive | 2004

FGF-2 derived proteins for the preparation of biomaterials or medical devices such as stents

Hervé Prats; Henrik Laurell; Carine Bossard; Barbara Garmy-Susini; Jean-François Arnal

Archive | 2018

Tableau d’insulinorésistance sévère chez une patiente présentant une mutation inactivatrice du récepteur des œstrogènes alpha

Eva Feigerlova; Henrik Laurell; Hervé Mittre; Marie-Laure Kottler; Marc Deshayes; Patrick Balaguer; William Bourget; Jean-François Arnal; Richard Marechaud; Samy Hadjadj; Pierre Gourdy

55th Annual ESPE | 2016

A Novel Homozygous Mutation in the Domain AF-2 of Alpha Estrogen Receptor Gene (ESR1), Generating a Bio-Inactive ER[alpha] Mutant, Resulting in Estrogen Resistance with Complex Metabolic Phenotype

Eva Feigerlova; Henrik Laurell; Hervé Mittre; Marie-Laure Kottler; Marc Deshayes; Patrick Balaguyer; Jean-François Arnal; Richard Marechaud; Samy Hadjadj; Pierre Gourdy

17th European Congress of Endocrinology | 2015

Roles of membrane oestrogen receptor alpha in bone sparing effects of oestrogens

Alexia Vinel; Coralie Fontaine; Eric Hay; Marie Cécile Valera; Françoise Lenfant; Martine Cohen-Solal; Jean-François Arnal

/data/revues/00034266/v74i2/S0003426613000280/ | 2013

Iconographies supplémentaires de l'article : In vivo dissection of the estrogen receptor alpha: Uncoupling of its physiological effects and medical perspectives

Jean-François Arnal; Pierre Gourdy; Françoise Lenfant

Archive | 2009

Intolerance in Mice Estrogens Protect against High-Fat Diet-Induced Insulin Resistance and Glucose

Elodie Riant; Aurélie Waget; Haude Cogo; Jean-François Arnal; Rémy Burcelin; Pierre Gourdy

Reproduction humaine et hormones | 2007

EFFETS DES STÉROÏDES SEXUELS ENDOGÈNES ET EXOGÈNES SUR LES VAISSEAUX ET LES FACTEURS DE RISQUE CARDIOVASCULAIRE

Pierre Gourdy; Jean-François Arnal

Reproduction humaine et hormones | 2004

Effets vasculaires des oestrogènes

Jean-François Arnal; Pierre Gourdy; Barbara Garmy-Susini; Laurent Brouchet; Francis Bayard

Archive | 2004

Proteines derivees du fgf-2 pour la preparation de biomateriaux ou de dispositifs medicaux de type endoprotheses vasculaires

Hervé Prats; Henrik Laurell; Carine Bossard; Barbara Garmy-Susini; Jean-François Arnal

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