Jennifer L. Moore

Relating foliar dehydration tolerance of mycorrhizal Phaseolus vulgaris to soil and root colonization by hyphae

Mycorrhizal symbiosis can modify plant response to drying soil, but little is known about the relative contribution of soil vs. root hyphal colonization to drought resistance of mycorrhizal plants. Foliar dehydration tolerance, characterized as leaf and soil water potential at the end of a lethal drying episode, was measured in bean plants (Phaseolus vulgaris) colonized by Glomus intraradices or by a mix of arbuscular mycorrhizal fungi collected from a semi-arid grassland. Path analysis modeling was used to evaluate how colonization rates and other variables affected these lethal values. Of several plant and soil characteristics tested, variation in dehydration tolerance was best explained by soil hyphal density. Soil hyphal colonization had larger direct and total effects on both lethal leaf water potential and soil water potential than did root hyphal colonization, root density, soil aggregation, soil glomalin concentration, leaf phosphorus concentration or leaf osmotic potential. Plants colonized by the semi-arid mix of mycorrhizal fungi had lower lethal leaf water potential and soil water potential than plants colonized by G. intraradices. Our findings support the assertion that external, soil hyphae may play an important role in mycorrhizal influence on the water relations of host plants.

Mycorrhizal promotion of host stomatal conductance in relation to irradiance and temperature

Colonization of roots and soil by arbuscular mycorrhizal (AM) fungi sometimes promotes stomatal conductance (gs) of the host plant, but scientists have had difficulty predicting or manipulating the response. Our objective was to test whether the magnitude of AM influence on gs is related to environmental conditions: irradiance, air temperature or leaf temperature. Stomatal conductances of two groups of uncolonized sorghum plants were compared to gs of plants colonized by Glomus intraradices (Gi) or Gigaspora margarita (Gm) in 31 morning and afternoon periods under naturally varying greenhouse conditions. Stomatal conductance of Gi and Gm plants was often markedly higher than gs of similarly sized nonAM plants. AM promotion of gs was minimal at the lowest irradiances and lowest air and leaf temperatures, but was substantial at intermediate irradiance and temperatures. AM promotion was again low or absent at the highest irradiances and temperatures. Magnitude of AM promotion of gs was not a function of absolute gs. Promotion of gs by Gi and Gm was remarkably similar. Differing phosphorus fertilization did not affect gs.

Comparative dehydration tolerance of foliage of several ornamental crops

Cultivars of Dahlia, Pentas, Salvia and two Impatiens were subjected to severe soil drying, and their foliar water relations were measured when fewer than eight live leaves remained (defined as the lethal point). Salvia was the most dehydration tolerant of the four genera, as characterized by lethal leaf water potential, and showed the highest osmotic adjustment. Dahlia and the two Impatiens cultivars had similar water relations at the lethal point. Length of the drying period, which was varied by growing plants in three pot sizes, did not affect any leaf water relations parameter. The paper also provides a ranking of the foliar dehydration tolerance of 25 other ornamental plants, measured in additional experiments. Foliage of woody species tended to be more tolerant of dehydration than foliage of herbaceous species. # 2003 Elsevier Science B.V. All rights reserved.