Jerome B. Smulow

An epithelial-like cell line in continuous culture from normal adult human gingiva.

Summary An epithelial-like cell line has been established from an area of clinically normal adult human attached gingiva, It has a generative time of approximately 24 hours in logarithmic phase and a plating efficiency of about 80-90%.

Healing of the periodontium following mucogingival surgery

Abstract A study was conducted on dogs to compare the outcome of mucogingival surgery with and without removal of the periosteum. The width of the attached gingiva following the surgical procedure could not be predetermined by removing the periosteum or leaving it intact. Removing the periosteum during mucogingival surgery delayed healing and caused a significant loss in the height of the alveolar bone.

The effect of gravity on initial microbial adhesion

An in vitro study was made of the initial microbial localization and adhesion of cultures of Streptococcus mutans 6715, Escherichia coli KB(ATCC-25922), whole saliva, and feces. Glass coverslips and glass, plastic, and stainless steel cylinders served as substrates. The results demonstrate that gravitational localization of the microbia is of primary importance in the adhesion process that follows.

Cultivation in vitro of Adult Human Skin and Oral Mucosa on Gelatin Film.

Summary Zones of epithelioid cells from single fragments of adult human skin and oral mucosa develop on gelatin film. Although the initial appearance of these cells was somewhat slower on this substrate, their subsequent migration or growth appeared to be comparable to that in plasma clot. Proliferation of fibroblast-like cells was observed in some skin cultures on gelatin film when the medium consisted of a modified Eagles basal solution supplemented with 15% calf serum but not when supplemented with 20% human or 10% human and 5% calf serum. Cultural characteristics of adult human skin and oral mucosa were similar except that fibroblast-like cells were more rarely observed and extensive sheaths of epithelioid cells occurred on glass as well as on gelatin film from oral mucosa.