Jerzy Klijanienko

Fine-needle sampling of salivary gland lesions IV. Review of 50 cases of mucoepidermoid carcinoma with histologic correlation

Fine‐needle sampling (FNS) of 50 mucoepidermoid carcinomas, including 44 primary tumors, five local recurrences, and one lymph node metastasis, was performed preoperatively in 44 patients. Concordant cytologic diagnoses were established in only 19 (38%) tumors, whereas 15 (30%) were classified as carcinoma, five (10%) as suspicious, and six (12%) as benign tumors. The material was insufficient for cytologic evaluation in five (10%) cases. The tumors were classified histologically as high‐, intermediate‐, and low‐grade in 15, 13, and 22 cases, respectively. The quality of diagnosis did not vary between high‐ and intermediate‐grade, but was lower in low‐grade tumors: Malignancy was diagnosed or suspected in 13 (87%) high‐grade tumors, 11 (85%) intermediate‐grade tumors, and 15 (68%) low‐grade tumors. In conclusion, FNS is an accurate technique in high‐ or intermediate‐grade mucoepidermoid carcinomas, but quite unsatisfactory in low‐grade tumors. Diagn. Cytopathol. 17:92–98, 1997.

Detection and quantitation by fluorescence in situ hybridization (FISH) and image analysis of HER-2/neu gene amplification in breast cancer fine-needle samples.

Fine‐needle sampling, although a practical and noninvasive method of tissue acquisition, has rarely been used for HER‐2/neu fluorescent in situ hybridization (FISH). To assess HER‐2/neu gene amplification in mammary carcinoma, FISH signals on cytology and corresponding tissue biopsies were detected visually and measured by image analysis. The results were correlated with patient and tumor characteristics.

Fine-needle sampling of salivary gland lesions III. cytologic and histologic correlation of 75 cases of adenoid cystic carcinoma: Review and experience at the Institut Curie with emphasis on cytologic pitfalls

Fine‐needle sampling (FNS) of 75 adenoid cystic carcinomas, including 44 primary tumors, 18 local recurrences, 10 lymph node, and 3 distant metastases, was performed in 66 patients. Concordant cytologic diagnoses were established in 68 tumors (90.7%), whereas 4 (5.4%) were classified as malignant (adenocarcinoma), 1 (1.3%) as suspicious, and 1 (1.3%) as pleomorphic adenoma. The material was insufficient for cytologic evaluation in 1 (1.3%) tumor. Adenoid cystic carcinoma is, in our opinion, easy to diagnose using the FNS technique. Diagn. Cytopathol. 17:36–41, 1997.

Fine‐needle sampling of salivary gland lesions V: Cytology of 22 cases of acinic cell carcinoma with histologic correlation

Fine‐needle sampling (FNS) of 22 acinic cell carcinomas, including 17 primary tumors, 4 local recurrences, and 1 lymph node metastasis was performed preoperatively in 17 patients. Cytologic diagnoses were concordant with histology in 3 (13.7%) cases, whereas 15 (68.2%) cases were cytologically classified as malignant, 2 (9.1%) as suspicious, and 1 (4.5%) as benign (pleomorphic adenoma). The material was unsatisfactory for cytologic evaluation in 1 (4.5%) case. Preoperative FNS technique is, therefore, useful in acinic cell carcinoma with a concordant malignant/suspicious rate of 91%. Diagn. Cytopathol. 1997;17:347–352.

Fine-needle sampling of salivary gland lesions I. Cytology and histology correlation of 412 cases of pleomorphic adenoma

Fine‐needle sampling (FNS) of 412 pleomorphic adenomas, corresponding to 378 primary tumors and 34 local recurrences, was performed preoperatively in 378 patients. Concordant cytologic diagnoses were established in 376 tumors (91.3%), whereas seven (1.7%) were only classified as benign. Sixteen (3.9%) tumors were considered to be suspicious and two (0.5%) were diagnosed as adenoid cystic carcinomas. Insufficient material for cytologic evaluation was found in 11 (2.6%) tumors. Pleomorphic adenomas were retrospectively classified according to the predominant histological differentiation: chondromyxoid, cellular, myoepithelial, or metaplastic. FNS performances were higher in chondromyxoid than in other types, with 95.2% concordant and 1.4% suspicious/false‐positive, vs. 81.8% concordant, and 11.5% suspicious/false‐positive cytodiagnoses, respectively. Diagn Cytopathol 1996;14:195–200.

Cytologic characteristics and histomorphologic correlations of 21 salivary duct carcinomas

Fine‐needle samplings (FNS) of 21 salivary duct carcinomas, histologically correlated, including 19 primaries, one local recurrence, and one lymph node metastasis from 19 patients, are reported. Cytologic diagnosis of high‐grade adenocarcinoma was established in 15 (71%). Five (24%) cases were misclassified as high‐grade mucoepidermoid carcinomas and one (5%) as squamous‐cell carcinoma. The histologic evaluation in all cases showed cytomorphologic features resembling mammary duct carcinoma with marked cytonuclear atypia and occasional oncocytic appearance. Our cytohistologic correlations indicate that irregular clusters of high‐grade adenocarcinoma cells with necrotic background and oncocytic features suggest a cytologic diagnosis of either primary salivary duct carcinoma or metastatic mammary carcinoma. Diagn. Cytopathol. 1998;19:333–337.

Fine‐needle sampling of salivary gland lesions II. Cytology and histology correlation of 71 cases of Warthin's tumor (adenolymphoma)

Fine‐needle sampling (FNS) of 71 Warthins tumors was performed preoperatively in 70 patients. Cytologic diagnoses were concordant with histology in 50 (70.4%) tumors, whereas 14 (19.7%) cases were cytologically misclassified as benign lesions. Material for cytologic evaluation was unsatisfactory in seven (9.9%) cases, and no suspicious or false‐positive diagnoses were observed. FNS is therefore a reliable preoperative technique for assessment of Warthins tumor with a concordant/benign diagnostic rate of 90.1%. Diagn. Cytopathol. 16:221–225, 1997.

Fine‐needle sampling findings in 26 carcinoma ex pleomorphic adenomas: Diagnostic pitfalls and clinical considerations

Preoperative fine‐needle samplings (FNS) of 26 histologically proven carcinoma ex pleomorphic adenomas (24 primary tumors and 2 local recurrences in 24 patients) were retrospectively reviewed in order to evaluate their cytologic characteristics. Histologically, 17 were high‐grade and 9 were low‐grade carcinomas; 10 carcinomas were intratumoral (in situ), and 16 were infiltrative.

Salivary carcinomas with papillae: Cytology and histology analysis of polymorphous low-grade adenocarcinoma and papillary cystadenocarcinoma

We retrospectively compared fine‐needle samplings (FNS) from two recently individualized low‐grade papillary salivary carcinomas, polymorphous low‐grade adenocarcinoma (PLGAC) and papillary cystadenocarcinoma (PCAC), to define the cytologic criteria allowing the correct diagnosis. Twelve PLGACs in 10 patients and 5 PCACs in 4 patients were investigated by FNS preoperatively. In both entities, smears contained variable proportions of malignant cells, occasionally arranged in pseudopapillary formations. In contrast to PCAC, PLGAC showed stromal fragments and hyaline globules resembling pleomorphic adenoma or adenoid cystic carcinoma.

Comparative cytologic and histologic study of fifteen salivary basal-cell tumors: differential diagnostic considerations.

Cytologic results of preoperative fine‐needle sampling (FNS) of 15 salivary basal‐cell tumors are presented, described, and compared with histologic results. Eleven of the FNAS showed individual and clusters of homogeneous basaloid cells with scanty cytoplasm, occasional peripheral palisading, and naked nuclei and were diagnosed as basal‐cell adenoma. Four samples showed, in addition, three‐dimensional cell clusters with mild cytonuclear atypia, occasional mitosis, and/or focal necrosis and were diagnosed as basal‐cell adenocarcinoma. Basal‐cell tumors must be diagnostically differentiated from adenoid cystic carcinoma and metastatic basal‐cell carcinoma. Although the adenomas diagnosed by cytologic examination and four suspected carcinomas in our series were verified by histologic testing, the bland cytologic features of basal‐cell adenocarcinoma may not always allow diagnosis on cytologic examination. Diagn. Cytopathol. 1999;21:30–34.